Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Characteristics of Viral Endothelial Cell Necrosis of Eel (VECNE) from Culturing Eel (Anguilla japonica, Anguilla bicolar) in Korea

국내 양식산 뱀장어(Anguilla japonica, Anguilla bicolar)에서 발생하는 바이러스성내피세포괴사증(VECNE)의 감염 특성

  • Kim, Su Mi (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Ko, Sang Mu (Pukyong National University Control Center for Aquatic Animal Disease) ;
  • Jin, Ji Hye (DaOn Fisheries Diseases management) ;
  • Seo, Jung Soo (Aquatic Life Disease Control Division, National Institute of Fisheries Science) ;
  • Lee, Nam Sil (Pathology Research Division, National Institute of Fisheries Science) ;
  • Kim, Young Suk (Aquatic Life Disease Control Division, National Institute of Fisheries Science) ;
  • Gu, Jeong Hui (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Bae, Yu Ri (West Sea Fisheries Research Institute, National Institute of Fisheries Science)
  • 김수미 (국립수산과학원 서해수산연구소) ;
  • 고상무 (부경대학교 수산질병관리원) ;
  • 진지혜 (다온 수산질병관리원) ;
  • 서정수 (국립수산과학원 수산방역과) ;
  • 이남실 (국립수산과학원 병리연구과) ;
  • 김영숙 (국립수산과학원 수산방역과) ;
  • 구정희 (국립수산과학원 서해수산연구소) ;
  • 배유리 (국립수산과학원 서해수산연구소)
  • Received : 2018.11.06
  • Accepted : 2018.12.08
  • Published : 2018.12.31
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Abstract

From 2017 to 2018, the disease has been monitored at four culturing eel farms in Incheon and Gyeonggi region in Korea. As a result, diseases with gill congestion frequently occurred. This disease occurred regardless of size of eel, but the frequency and cumulative mortality were high in eels within 3 months after stocking. The infected fish showed pathological histopathological features such as intense congestion and dilation in the central venous sinus (CVS) of gill filaments and hemorrhages in liver and kidney. Hexagonal viral particles measuring about 70 nm in diameter was observed in nuclei and cytoplasm of gill vascular endothelial cells. Molecular biologic diagnosis by both PCR and genetic analysis has been revealed that the causative agent of this disease is Japanese eel endothelial cells-infecting virus (JEECV), the cause of viral endothelial cell necrosis of eel (VECNE), which is mainly reported in Japan. This study is the first report on the characteristics of JEECV and VECNE infection in domestic eel farms.

2017~2018년에 거쳐 인천 및 경기지역 뱀장어 양식장 4개소를 대상으로 질병 예찰 및 모니터링을 실시한 결과, 특징적으로 아가미울혈증상을 동반한 질병이 빈번히 발생하였다. 이 질병은 크기에 상관없이 발생하지만 입식 후 3개월 이내의 당년생 뱀장어에서 감염 빈도와 누적 폐사율이 높게 나타났다. 감염어는 새변의 중심정맥동(CVS)의 심한 울혈과 팽창, 간과 신장 조직의 출혈 등과 같은 병리조직학적 감염 특성을 보였다. 또한 아가미 혈관내피세포의 핵과 세포질에서 정이십면체 구조를 가진 직경 70 nm 전후의 바이러스 입자가 관찰되었다. PCR에 의한 분자생물학적 진단법과 유전자 분석을 통해 이 질병의 원인 병원체는 일본에서 주로 보고한 뱀장어바이러스성내피세포괴사증(VECNE)의 원인체인 JEECV로 밝혀졌다. 본 연구는 국내 뱀장어 양식장에서 JEECV 및 VECNE 감염특성을 밝힌 첫 보고이다.

Keywords

References

  1. Blake, S.L., W.B. Schill, P.E. McAllister, M.K. Lee, J.T. Singer and B.L. Nicholson. 1995. Detection and identification of aquatic birnaviruses by PCR assay. J. Clin. Microbiol., 33: 835-839.
  2. Egusa, S., M. Tanaka, H. Ogami and H. Oka. 1989. Histopathological observations on an intense congestion of the gills in cultured Japanese eel, Anguilla japonica. Fish Pathol., 24: 51-56. (in Japanese) https://doi.org/10.3147/jsfp.24.51
  3. Haenen, O., I. Mladineo, R. Konecny, M. Yoshimizu, D. Groman, P. Munoz, A. Saraiva, S. Bergmann and S. Van Beurden. 2012. Diseases of eels in an international perspective: Workshop on eel diseases at the 15th international conference on diseases of fish and shellfish, split, croatia, 2011. Bull. Eur. Assoc. Fish Pathologists, 32: 109-115.
  4. Inouye, K., S. Miwa, H. Aoshima, H. Oka and M. Sorimachi. 1994. A histopathological study on the etiology of intense congestion of the gills of Japanese eel, Anguilla japonica. Fish Pathol., 29: 35-41. https://doi.org/10.3147/jsfp.29.35
  5. Jennifer, A.D., F.F.N. Terry and C.C. Alvin. 2016. Complete sequence of the smallest polyomavirus genome, giant guitarfish (Rhynchobatus djiddensis) polyomavirus 1. Genome Announc., 4: e00391-16.
  6. Kim, Y.G., M.S. Choi, S.H. Park and K.K. Lee. An investigation of the congestion on the gills of eel. J. Fish Pathol., 7: 161- 171. (in Korean)
  7. Mizutani, T., Y. Sayama, A. Nakanishi, H. Ochiai, K. Sakai, K. Wakabayashi, N. Tanaka, E. Miura, M. Oba, I. Kurane, M. Saijo, S. Morikawa and S. Ono. 2011. Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica. Virology, 412: 179-187. https://doi.org/10.1016/j.virol.2010.12.057
  8. Moens, U., A. Krumbholz, B. Ehlers, R. Zell, R. Johne, S. Calvignac-Spencer and C. Lauber. 2017. Biology, evolution, and medical importance of polyomaviruses: an update. Infect. Genet. Evol., 54: 18-38. https://doi.org/10.1016/j.meegid.2017.06.011
  9. Okazaki, S., S. Yasumoto, S. Koyama, S. Tsuchiaka, Y. Naoi, T. Omatsu, S. Ono and T. Mizutani. 2016. Detection of Japanese eel endothelial cells-infecting virus in Anguilla japonica elvers. J. Vet. Med. Sci., 78: 705-707. https://doi.org/10.1292/jvms.15-0515
  10. Ono, S. and A. Nagai. 1997. Electronmicroscopic observation and experimental infection of congestion in gills of Japanese eel, Anguilla japonica. J. School Marine Sci. Technol., 43: 95-105.
  11. Ono, S., K. Wakabayashi and A. Nagai. 2007. Isolation of the virus causing viral endothelial cell necrosis of eel from cultured Japanese eel, Anguilla japonica. Fish Pathol., 42: 191-200. (in Japanese) https://doi.org/10.3147/jsfp.42.191
  12. Park, S.W., E.B. Jung and D.W. Kim. 2012. Outbreak of anguillid herpesvirus-1 (AngHV-1) infection in cultured shortfin eel (Anguilla bicolor) in Korea. J. Fish Pathol., 25: 151-158. (in Korean) https://doi.org/10.7847/jfp.2012.25.3.151
  13. Peretti, A., P.C. FitzGerald, V. Bliskovsky, D.V. Pastrana and C.B. Buck. 2015. Genome sequence of a fish associated polyomavirus, black sea bass (Centropristis striata) polyomavirus 1. Genome Announc., 3: e01476-13.
  14. Rijsewijk, F., S. Pritz-Verschuren, S. Kerkhoff, A. Botter, M. Willemsen, A. van Nieuwstadt and O. Haenen. 2005. Development of a polymerase chain reaction for the detection of Anguillid herpesvirus DNA in eels based on the herpesvirus DNA polymerase gene. J. Virol. Methods, 124: 87-94. https://doi.org/10.1016/j.jviromet.2004.11.007
  15. Tanaka, M., T. Satoh, W.J. Ma and S. Ono. 2008. Effectiveness of increasing temperature of rearing water and non-feeding against viral endothelial cell necrosis of eel. Fish Pathol., 43: 79-82. https://doi.org/10.3147/jsfp.43.79
  16. van Beurden, S.J., M.A. Voorbergen-Laarman, I. Roozenburg, A.S. Boerlagea, O.L.M. Haenen and M.Y. Engelsma. 2011. Development and validation of a two-step real-time RT-PCR for the detection of eel virus European X in European eel, Anguilla anguilla. J. Virol. Metohods, 171: 352-359. https://doi.org/10.1016/j.jviromet.2010.11.019
  17. Wen, C.M., M.M. Chen, C.S. Wang, P.C. Liu and F.H. Nan. 2015. Isolation of a novel polyomavirus, related to Japanese eel endothelial cell-infecting virus, from marbled eels, Anguilla marmorata (Quoy & Gaimard). J. Fish Dis., 39: 889-897.
  18. Wen, C.M. 2017. Complete genome sequence and phylogenetic analyses of an aquabirnavirus isolated from a diseased marbled eel culture in Taiwan. Arch. Virol., 162: 2467-2471. https://doi.org/10.1007/s00705-017-3382-5