Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Breeding a new white button mushroom cultivar 'Dodam'

백색 양송이 신품종 '도담' 육성

  • Oh, Youn-Lee (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Nam, Noun-keol (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jang, Kab-Yeul (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Oh, MinJi (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Im, Ji-Hoon (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Seul-ki (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
  • 오연이 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 남윤걸 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오민지 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 임지훈 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 이슬기 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2018.09.07
  • Accepted : 2018.12.10
  • Published : 2018.12.31
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Abstract

The button mushroom, Agaricus bisporus, is one of the most widely cultivated mushrooms. The domestic production of cultivated mushrooms in 2016 was approximately 10,173 tons, with a total value of 59 billion won. Currently, 10 cultivars have been developed; however, Korean farmers continue to demand new cultivars of mushrooms with improved quality. To breed superior lines, KMCC00754 and KMCC00775 were selected as mother strains from the 170 collected genetic resources. The putative homokaryotic strains were 25 strains from the 120 SSIs of KMCC00754 and six strains from the 120 SSIs of KMCC00775 selected by using the AbSSR45 marker. These homokaryotic strains were crossed with each other and the crossing was confirmed by SSR analysis. Seventy-four lines were crossed into 150 lines for a 50% ratio of crossing. Abs2-2015-16 was selected as a superior line by three cultivations. A new cultivar, 'Dodam', was developed in 2017.

양송이는 전 세계적으로 가장 많이 재배하는 버섯으로 특용작물생산실적의 2016년 통계자료에 의하면 국내 생산량은 10,173톤, 국내 생산액은 590억원이다. 현재 10개의 품종이 개발되었지만, 농업인들은 계속적으로 품질이 우수한 버섯 신품종을 요구하고 있다. 이에 우량계통을 육성하기 위해 수집된 170개의 유전자원 중에서 KMCC00754와 KMCC00775은 모균주로 선발되었다. 그리고 KMCC00754에서 120점의 단포자에서 동형핵균주로 추정되는 균주를 25점 선발하였고, KMCC00775에서는 120점의 단포자에서 6점을 AbSSR45마커를 가지고 각각 선발하였다. 이 동형핵균주로 교잡주를 육성하여 다시 SSR마커로 분석하였다. 그 결과 50%의 교잡율로 150점의 교잡주에서 74점이 교잡된 것을 확인하였으며, 이 균주들을 3회 재배하고 특성을 평가하였다. 최종적으로 Abs2-2015-16을 우량계통으로 선발하여 2017년에 신품종 '도담'이 육성되었다.

Keywords

BSHGBD_2018_v16n4_279_f0001.png 이미지

Fig 1. The selection of the homokaryons using the AbSSR45 marker (Left KMCC00754; Right KMCC00775).

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Fig 2. The confirmation of the crossing with homokaryons(KMCC00754 and KMCC00775) by the capillary electrophoresis (M: 1kb marker; 1: KMCC00754-11(homokaryon) ; 2: KMCC00775-12((homokaryon) ; 3: KMCC00754-11XKMCC00775-12(crossed line) ; 4: KMCC00754-12 ; 5: KMCC00775-26 ; 6: KMCC00754-12XKMCC00775-26 ; 7: KMCC00754-78 ; 8: KMCC00775-12 ; 9: KMCC00754-78XKMCC00775-12 ; 10: KMCC00754-78 ; 11: KMCC00775-26 ; 12: KMCC00754-78XKMCC00775-26 ; 13: KMCC00754-91 ; 14: KMCC00775-48 ; 15: KMCC00754-91XKMCC00775-48 ; 16: KMCC00754-91 ; 17: KMCC00775-52 ; 18: KMCC00754-91XKMCC00775-52).

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Fig 3. The band size of the PCR products using the AbSSR45 marker.

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Fig 4. Selection of superior line through three button mushroom cultivations (A: Superior line in 1st box cultivation; B Superior line in 3rd block cultivation; C Superior line in mushroom farm)

Table 1. The list of primer in this study

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Table 1. The list of primer in this study

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Table 2. The agronomic and morphological characteristics of the selected strains

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Table 3. The list of the crossed lines with homokaryons of KMCC00754 and homokaryons of KMCC00775.

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Table 4. The agronomic and morphological characteristics of the selected strains in the crossed lines.

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