Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Yield characteristics of Pleurotus ostreatus according to the use of spent mushroom substrate with high nitrogen content

질소원이 증진된 수확후배지를 이용한 느타리버섯 수량 특성

  • Baek, Il-Sun (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Kim, Jeong-Han (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Lee, Yong-Seon (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Shin, Bok-Eum (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Lee, Yun-Hae (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Lee, Young-Soon (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services)
  • 백일선 (경기도농업기술원 버섯연구소) ;
  • 김정한 (경기도농업기술원 버섯연구소) ;
  • 이용선 (경기도농업기술원 버섯연구소) ;
  • 신복음 (경기도농업기술원 버섯연구소) ;
  • 이윤혜 (경기도농업기술원 버섯연구소) ;
  • 이영순 (경기도농업기술원 버섯연구소)
  • Received : 2018.11.30
  • Accepted : 2018.12.20
  • Published : 2018.12.31
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Abstract

The aim of this study was to re-use spent mushroom substrate (SMS) with increased total nitrogen (T-N) and amino acid content and reduce the amount of cottonseed meal used as nutrient supplement in Pleurotus ostreatus cultivation. Bacteria used for improvement of the T-N content were GM20-4(Bacillus sp.) and Rhodobacter sphaeroides (RS). GM20-4 was isolated from the SMS of P. ostreatus and RS was obtained from Gwangjusi agricultural technology center. SMS in T1, T2, and T3 was reused as substrate after drying and the T-N content of dried SMS (D-SMS) was increased by 0.34% by treatment with the bacteria. T1 with 8% D-SMS and T2 with 18% D-SMS had higher rates of primordia formation compared with T3 and the control. The biological efficiency of the control and of treatment with 8%, 18%, and 26% D-SMS was 110%, 114%, 112%, and 79%, respectively. Considering the economic cost, yield, and biological efficiency, T2 with 18% D-SMS as the culture substrate for P. ostreatus was shown to be the most effective for cultivation.

본 연구는 아미노산과 질소원이 증진된 수확후배지를 재사용하고, 느타리에서 영양원으로 사용되는 면실박의 사용을 줄이기 위해 수행되었다. 수확후배지의 질소원 증진을 위해 두 가지의 세균이 사용 되었으며, GM20-4는 느타리버섯의 수확후배지부터 분리되었고, Rhodobacter sphaeroides는 광주시농업기술 센터로부터 분양받았다. 처리구에 사용된 수확후배지는 건조 후 사용했으며, 위의 2가지의 미생물 처리에 의해 수확후배지의 총질소 함량은 0.34% 증가되었다. 8% D-SMS 가진 T1처리구와 18% D-SMS가 첨가된 T2가 대조와 T3처리구보다 발이율이 높았으며, 생물학적 효율은 대조 110%, T1이 114%, T2가 112%, T3가 79%로 조사되었다. 경제성, 수량 및 생물학적 효율을 고려해볼 때 18% 건조 수확후배지를 사용한 T2가 느타리 재배배지로 가장 효율적인 것으로 조사되었다.

Keywords

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Fig. 1. Colony morphology of the bacteria used in this study

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Fig. 2. Analysis of amino acid content according to the treatment of GM20-4 and RS in D-SMS C : D-SMS + molasses 3%, T : D-SMS + GM20-4 30% + RS 30% + molasses 3%

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Fig. 3. Fruit body in Pleurotus ostreatus according to substrate composition

Table 1. Chemical properties of material used in this study

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Table 2. Cultivation condition of the two bacteria used in this study

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Table 3. Change of T-N according to the treatment of bacteria in dried spent mushroom substrates(D-SMS)

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Table 4. Mix ratio of materials according to the treatment

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Table 5. Chemical properties according to the substrates composition

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Table 6. Cultural properties in Pleurotus ostreatus according to the substrate composition

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Table 7. Fruit body characteristics in Pleurotus ostreatus according to substrate composition

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Table 8. Analysis of economical costs and according to the substrate composition of treatment

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