Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences

  • Gou, Huitian (College of Veterinary Medicine, Gansu Agricultural University) ;
  • Xue, Huiwen (College of Veterinary Medicine, Gansu Agricultural University) ;
  • Yin, Hong (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Science) ;
  • Luo, Jianxun (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Science) ;
  • Sun, Xiaolin (College of Veterinary Medicine, Gansu Agricultural University)
  • Received : 2018.02.02
  • Accepted : 2018.04.25
  • Published : 2018.12.31
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Abstract

Although widely studied, the natural diversity of the hard tick is not well known. In this study, we collected 194 sequences from 67 species, covering 7 genera of hard tick. The 5' region of the mitochondrial cytochrome c oxidase subunit 1 region (586 bp) has been used to investigate intra- and inter-species variation and the phylogenetic tree of neighbor joining method has been used for assessment. As a result, by comparing the K2P-distance of intra- and interspecies, 30 samples (15.2%) shown that interspecies distance was larger than the minimum interspecfic distance. From the phylogenetic analysis, 86.8% (49) of the species were identified correctly at the genus level. On deeper analysis on these species suggested the possibility of presence cryptic species. Therefore, further work is required to delineate species boundaries and to develop a more complete understanding of hard tick diversity over larger scale.

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