Parasites, Hosts and Diseases

  • 제56권6호
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  • Pages.545-552
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  • 2018
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Genetic Diversity of Plasmodium vivax Causing Epidemic Malaria in the Republic of Korea

  • Bahk, Young Yil (Department of Biotechnology, College of Biomedical and Health Science, Konkuk University) ;
  • Kim, Jeonga (Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, UAB Comprehensive Diabetes Center, University of Alabama at Birmingham) ;
  • Ahn, Seong Kyu (Department of Parasitology and Tropical Medicine, Inha University School of Medicine) ;
  • Na, Byoung-Kuk (Department of Parasitology and Tropical Medicine and Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • Chai, Jong-Yil (Korea Association of Health Promotion) ;
  • Kim, Tong-Soo (Department of Parasitology and Tropical Medicine, Inha University School of Medicine)
  • 투고 : 2018.09.03
  • 심사 : 2018.11.05
  • 발행 : 2018.12.31
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초록

Plasmodium vivax is more challenging to control and eliminate than P. falciparum due to its more asymptomatic infections with low parasite densities making diagnosis more difficult, in addition to its unique biological characteristics. The potential re-introduction of incidence cases, either through borders or via human migrations, is another major hurdle to sustained control and elimination. The Republic of Korea has experienced re-emergence of vivax malaria in 1993 but is one of the 32 malaria-eliminating countries to-date. Despite achieving successful nationwide control and elimination of vivax malaria, the evolutionary characteristics of vivax malaria isolates in the Republic of Korea have not been fully understood. In this review, we present an overview of the genetic variability of such isolates to increase understanding of the epidemiology, diversity, and dynamics of vivax populations in the Republic of Korea.

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참고문헌

  1. Feachem RG, Phillips AA, Hwang J, Cotter C, Wielgosz B, Greenwood BM, Sabot O, Rodriguez MH, Abeyasinghe RR, Ghebreyesus TA, Snow RW. Malaria elimination 1: Shrinking the malaria map: progress and prospects. Lancet 2010; 376: 1566-1578. https://doi.org/10.1016/S0140-6736(10)61270-6
  2. WHO. World malaria report. Geneva: World Health Organization 2017.
  3. Mendis K, Sina BJ, Marchesini P, Carter R. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 2001; 64: 97-106. https://doi.org/10.4269/ajtmh.2001.64.97
  4. Carlton JM, Sina BJ, Adams JH. Why is Plasmodium vivax a neglected tropical disease? PLoS Negl Trop Dis 2011; 5: e1160. https://doi.org/10.1371/journal.pntd.0001160
  5. Luo Z, Sullivan SA, Carlton JM. The biology of Plasmodium vivax explored through genomics. Ann NY Acad Sci 2015; 1342: 53-61. https://doi.org/10.1111/nyas.12708
  6. Price RN, Tjitra E, Guerra CA, Yeung S, White NJ, Anstey NM. Vivax malaria: neglected and not benign. Am J Trop Med Hyg 2007; 77: 79-87. https://doi.org/10.4269/ajtmh.2007.77.79
  7. Mueller I, Galinski MR, Baird JK, Carlton JM, Kochar DK, Alonso PL, del Portillo HA. Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite. Lancet Infect Dis 2009; 9: 555-566. https://doi.org/10.1016/S1473-3099(09)70177-X
  8. Ashley EA, Pyae PA, Woodrow CJ. Malaria. Lancet 2018; 391: 1608-1621. https://doi.org/10.1016/S0140-6736(18)30324-6
  9. Bahk YY, Lee HW, Na BK, Kim J, Jin K, Hong YS, Kim TS. Epidemiological characteristics of re-emerging vivax malaria in the Repubic of Korea. Korean J Parasitol 2018; 56(6): 531-543. https://doi.org/10.3347/kjp.2018.56.6.531
  10. Chai JY. Re-emerging Plasmodium vivax malaria in the Republic of Korea. Korean J Parasitol 1999; 37: 129-143. https://doi.org/10.3347/kjp.1999.37.3.129
  11. Guerra CA Howes RE, Patil AP, Gething PW, Van Boeckel TP, Temperley WH, Kabaria CW, Tatem AJ, Manh BH, Elyazar IR, Baird JK, Snow RW, Hay SI. The international limits and population at risk of Plasmodium vivax transmission in 2009. PLoS Negl Trop Dis 2010; 4: e774. https://doi.org/10.1371/journal.pntd.0000774
  12. Schultz L, Wapling J, Mueller I, Ntsuke PO, Senn N, Nale J, Kiniboro B, Buckee CO, Tavul L, Siba PM, Reeder JC, Barry AE. Multilocus haplotypes reveal levels of diversity and population structure of Plasmodium falciparum in Papua New Guinea, a region of intense perennial transmission. Malar J 2010; 9: 336. https://doi.org/10.1186/1475-2875-9-336
  13. Cui L, Escalante AA, Imwong M, Snounou G. The genetic diversity of Plasmodium vivax populations. Trends Parasitol 2003; 19: 220-226. https://doi.org/10.1016/S1471-4922(03)00085-0
  14. Rich SM, Ayala FJ. Population structure and recent evolution of Plasmodium falciparum. Proc Natl Acad Sci USA 2000; 97: 6994-7001. https://doi.org/10.1073/pnas.97.13.6994
  15. Chai IH, Lim GI, Yoon SN, Oh WI, Kim SJ, Chai JY. Occurrence of tertian malaria in a male patient who has never been abroad. Korean J Parasitol 1994; 32: 195-200 (in Korean). https://doi.org/10.3347/kjp.1994.32.3.195
  16. Lee KJ, Kim CB, Choi BJ, Park KH, Park JK. Analysis of vivax malaria cases in Gangwon-do (province), Korea in the year 2000. Korean J Parasitol 2001; 39: 301-306. https://doi.org/10.3347/kjp.2001.39.4.301
  17. Moon JJ, Cho SY. Incidence patterns of vivax malaria I civilians residing in a high-risk county of Kyonggi-do (province), Republic of Korea. Korean J Parasitol 2001; 39: 293-299. https://doi.org/10.3347/kjp.2001.39.4.293
  18. Lee JS, Lee WJ, Cho SH, Ree HI. Outbreak of vivax malaria in areas adjacent to the demilitarized zone, South Korea. Am J Trop Med Hyg 2002; 66: 13-17. https://doi.org/10.4269/ajtmh.2002.66.13
  19. Nkhoma SC, Nair S, Al-Saai S, Ashley E, McGready R, Phyo AP, Nosten F, Anderson TJ. Population genetic correlates of declining transmission in a human pathogen. Mol Ecol 2013; 22: 273-285. https://doi.org/10.1111/mec.12099
  20. Roy SW, Ferreira MU, Hartl DL. Evolution of allelic dimorphism in malarial surface antigens. Heredity 2008; 100: 103-110. https://doi.org/10.1038/sj.hdy.6800887
  21. Barry AE, Arnott A. Strategies for designing and monitoring malaria vaccines targeting diverse antigens. Front Immunol 2014; 5: 359.
  22. Barry AE, Waltmann A, Koepfli C, Barnadas C, Mueller I. Uncovering the transmission dynamics of Plasmodium vivax using population genetics. Pathog Glob Health 2015; 109: 142-152. https://doi.org/10.1179/2047773215Y.0000000012
  23. Auburn S, Barry AE. Dissecting malaria biology and epidemiology using population genetics and genomics. Int J Parasitol 2017; 47: 77-85. https://doi.org/10.1016/j.ijpara.2016.08.006
  24. Casares S, Richie TL. Immune evasion by malaria parasites: a challenge for vaccine development. Curr Opin Immunol 2009; 21: 321-330. https://doi.org/10.1016/j.coi.2009.05.015
  25. Suwanarusk R, Russell B, Chavchich M, Chalfein F, Kenangalem E, Kosaisavee V, Prasetyorini B, Piera KA, Barends M, Brockman A, Lek-Uthai U, Anstey NM, Tjitra E, Nosten F, Cheng Q, Price RN. Chloroquinine resistant Plasmodium vivax: in vitro characterization and association with molecular polymorphisms. PLoS One 2007; 2: e1089. https://doi.org/10.1371/journal.pone.0001089
  26. Huh AJ, Kwak YG, Kim ES, Lee KS, Yeom JS, Cho YK, Kim CS, Park JW. Parasitaemia characteristics of Plasmodium vivax malaria patients in the Republic of Korea. J Korean Med Sci 2011; 26: 42-46. https://doi.org/10.3346/jkms.2011.26.1.42
  27. Oh MD, Shin H, Shin D, Kim U, Lee S, Kim N, Choi MH, Chai JY, Choe K. Clinical features of vivax malaria. Am J Trop Med Hyg 2001; 65: 143-146. https://doi.org/10.4269/ajtmh.2001.65.143
  28. Kim JY, Goo YK, Zo YG, Ji SY, Trimarsanto H, To S, Clark TG, Price RN, Auburn S. Further evidence of increasing diversity of Plasmodium vivax in the Republic of Korea in recent years. PLoS One 2016; 11: e0151514. https://doi.org/10.1371/journal.pone.0151514
  29. Neafsey DE, Galinsky K, Jiang RH, Young L, Sykes SM, Saif S, Gujja S, Goldberg JM, Young S, Zeng Q, Chapman SB, Dash AP, Anvikar AR, Sutton PL, Birren BW, Escalante AA, Barnwell JW, Carlton JM. The malaria parasite Plasmodium vivax exhibits greater genetic diversity than Plasmodium falciparum. Nat Genet 2012; 44: 1046-1050. https://doi.org/10.1038/ng.2373
  30. Choi YK, Choi KM, Park MH, Lee EG, Kim YJ, Lee BC, Cho SH, Rhie HG, Lee HS, Yu JR, Lee JS, Kim TS, Kim JY. Rapid dissemination of newly introduced Plasmodium vivax genotypes in South Korea. Am J Trop Med Hyg 2010; 82: 426-432. https://doi.org/10.4269/ajtmh.2010.09-0245
  31. Arnott A, Barry AE, Reeder JC. Understanding the population genetics of Plasmodium vivax is essential for malaria control and elimination. Malar J 2012; 11: 14. https://doi.org/10.1186/1475-2875-11-14
  32. Brito CF, Ferreira MU. Molecular markers and genetic diversity of Plasmodium diversity of Plasmodium vivax. Mem Inst Oswaldo Cruz 2011; 106: 12-26. https://doi.org/10.1590/S0074-02762011000900003
  33. Hwang SY, Kim SH, Kho WG. Genetic characteristics of polymorphic antigenic markers among Korean isolates of Plasmodium vivax. Korean J Parasitol 2009; 47 (suppl): 51-58. https://doi.org/10.3347/kjp.2009.47.S.S51
  34. Kho W, Chung J, Sim E, Kim D, Chung W. Analysis of polymorphic regions of Plasmodium vivax Duffy binding protein of Korean isolates. Korean J Parasitol 2001; 39: 143-150. https://doi.org/10.3347/kjp.2001.39.2.143
  35. Lim CS, Kim SH, Kwon SI, Song JW, Song KJ, Lee KN. Analysis of Plasmodium vivax merozoite surface protein-1 gene sequences from resurgent Korean isolates. Am J Trop Med Hyg 2000; 62: 261-265. https://doi.org/10.4269/ajtmh.2000.62.261
  36. Han ET, Song TE, Park JH, Shin EH, Guk SM, Kim TY, Chai JY. Allelic dimorphism in the merozoite surface protein-$3{\alpha}$ in Korean isolates of Plasmodium vivax. Am J Trop Med Hyg 2004; 71: 745-749. https://doi.org/10.4269/ajtmh.2004.71.745
  37. Han ET, Park JH, Shin EH, Choi MH, Oh MD, Chai JY. Apical membrane antigen-1 (AMA-1) gene sequences of re-emerging Plasmodium vivax in South Korea. Korean J Parasitol 2002; 40: 157-162. https://doi.org/10.3347/kjp.2002.40.3.157
  38. Suh IB, Hoffman KJ, Kim SH, Song KJ, Song JW, Lee JS, Lim CS. The analysis of Plasmodium vivax Duffy receptor binding domain gene sequence from resurgent Korea isolates. Parasitol Res 2001; 87: 1007-1010.
  39. Kang JM, Lee J, Cho PY, Moon SU, Ju HL, Ahn SK, Sohn WM, Lee HW, Kim TI, Na BK. Population genetic structure and natural selection of apical membrane antigen-1 in Plasmodium vivax Korean isolates. Malar J 2015; 14: 455. https://doi.org/10.1186/s12936-015-0942-6
  40. Snewin VA, Premawansa S, Udagama PV, Mattei DM, Khouri E, Del Giudice G, Mendis KN, David PH. Transmission blocking immunity in Plasmodium vivax malaria: Antibodies raised against a peptide block parasite development in the mosquito vector. J Exp Med 1995; 181: 357-362. https://doi.org/10.1084/jem.181.1.357
  41. Kho WG, Chung JY, Hwang UW, Chun JH, Park YH, Chung WC. Analysis of polymorphic region of GAM-1 gene in Plasmodium vivax Korean isolates. Korean J Parasitol 2001; 39: 313-318. https://doi.org/10.3347/kjp.2001.39.4.313
  42. Choi YY, Choi KM, Park M-H, Lee EG, Kim YJ, Lee BC, Cho SH, Rhie HG, Lee HS, Yu JR, Lee JS, Kim TS, Kim JY. Rapid dissemination of newly introduced Plasmodium vivax genotypes in South Korea. Am J Trop Med Hyg 2010; 82: 426-432. https://doi.org/10.4269/ajtmh.2010.09-0245
  43. Nardin EH, Zavala F. Acquired immunity to sporozoites. In Sherman IW, Malaria: Parasite Biology, Pathogenesis, and Protection. Washington DC, USA. ASM Press. 1998, pp 495-511.
  44. Nam HW, Song KJ, Ahn HJ, Yang Z, Chong CK, Cho PY, Ahn SK, Kim TS. Probability of antibody formation against circumsporozoite protein of Plasmodium vivax among Korean malaria patients. Korean J Parasitol 2014; 52: 143-149. https://doi.org/10.3347/kjp.2014.52.2.143
  45. Kang JM, Lee J, Cho PY, Kim TI, Sohn WM, Park JW, Kim TS, Na BK. Dynamic changes of Plasmodium vivax population structure in South Korea. Infect Genet Evol 2016; 45: 90-94. https://doi.org/10.1016/j.meegid.2016.08.023
  46. Ju HL, Kang JM, Moon SU, Bahk YY, Cho PY, Sohn WM, Park YK, Park JW, Kim TS, Na BK. Genetic diversity and natural selection of Duffy binding protein of Plasmodium vivax Korean isolates. Acta Trop 2013; 125: 67-74. https://doi.org/10.1016/j.actatropica.2012.09.016
  47. Shin HI, Kim JY, Lee WJ, Sohn Y, Lee SW, Kang YJ, Lee HW. Polymorphism of the parasite lactate dehydrogenase gene from Plasmodium vivax Korean isolates. Malar J 2013; 12: 166. https://doi.org/10.1186/1475-2875-12-166
  48. Kim JY, Goo YK, Zo YG, Ji SY, Trimarsanto H, To S, Clark TG, Price RN, Auburn S. Further evidence of increasing diversity of Plasmodium vivax in the Republic of Korea in recent years. PLoS One 2016; 11: e0151514. https://doi.org/10.1371/journal.pone.0151514
  49. Hisaeda H, Yasutomo K, Himeno K. Malaria: immune evasion by parasites. Int J Biochem Cell Biol 2005; 37: 700-706. https://doi.org/10.1016/j.biocel.2004.10.009
  50. Ferreira MU, Karunaweera ND, da Silva-Nunes M, da Silva NS, Wirth DF, Hartl DL. Population structure and transmission dynamics of Plasmodium vivax in rural Amazonia. J Infect Dis 2007; 195: 1218-1226. https://doi.org/10.1086/512685
  51. Russell B, Suwanarusk R, Lek-Uthai U. Plasmodium vivax genetic diversity: microsatellite length matters. Treands Parasitol 2006; 22: 399-401. https://doi.org/10.1016/j.pt.2006.06.013
  52. Su X, Ferdig MT, Huang Y, Huynh CQ, Liu A, You J, Wootton JC, Wellems TE. A genetic map and recombination parameters of the human malaria parasite Plasmodium falciparum. Science 1999; 286: 1351-1353. https://doi.org/10.1126/science.286.5443.1351
  53. Karrunaweera ND, Ferreira MU, Hartl DL, Wirth DF. Fourteen polymorphic microsatellite DNA markers for the human malaria parasite Plasmodium vivax. Mol Ecol Notes 2007; 7: 172-175.
  54. Rezende AM, Tarazona-Santos E, Fontes CJ, Souza JM, Couto AD, Carvalho LH, Brito CF. Microsatellite loci: determining the genetic variability of Plasmodium vivax. Trop Med Int Health 2010; 15: 718-726. https://doi.org/10.1111/j.1365-3156.2010.02535.x
  55. Schlotterer C. Genome evolution: are microsatellites really simple sequences? Curr Biol 1998; 8: 132-134. https://doi.org/10.1016/S0960-9822(98)70989-3
  56. Gomez JC, Bockarie MJ, Baird JK, Carlton JM, Zimmerman PA. Identification of a polymorphic Plasmodium vivax microsatellite marker. Am J Trop Med Hyg 2003; 69: 377-379. https://doi.org/10.4269/ajtmh.2003.69.377
  57. Carlton JM, Galinski MR, Barnwell JW, Dame JB. Karyotype and synteny among the chromosomes of all four species of human malaria parasite. Mol Biochem Parasitol 1999; 101: 23-32. https://doi.org/10.1016/S0166-6851(99)00045-6
  58. Iwagami M, Fukumoto M, Hwang SY, Kim SH, Kho WG, Kano S. Population structure and transmission dynamics of Plasmodium vivax in the Republic of Korea based on microsatellite DNA analysis. PLoS Negl Trop Dis 2012; 6: e1592. https://doi.org/10.1371/journal.pntd.0001592
  59. Iwagami M, Hwang SY, Kim SH, Park SJ, Lee GY, Louise E, Matsumoto-Takahashi A, Kho WG, Kano S. Microsatellite DNA analysis revealed a drastic genetic change of Plasmodium vivax population in the Republic of Korea during 2002 and 2003. PLoS Negl Trop Dis 2013; 10: e2522.
  60. Yeom JS, Ryu SH, Oh S, Choi DH, Song KJ, Oh YH, Lee JH, Kim YA, Ahn SY, Yang HY, Cha JE, Park JW. Evaluation of anti-malarial effects of mass chemoprophylaxis in the Republic of Korea. J Korean Med Sci 2005; 20: 707-712. https://doi.org/10.3346/jkms.2005.20.5.707
  61. Chung DI, Jeong S, Dinzouna-Boutamba SD, Yang HW, Yeo SG, Hong Y, Goo YK. Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodium vivax isolates from Republic of Korea military personnel. Malar J 2015; 14: 336. https://doi.org/10.1186/s12936-015-0845-6
  62. Imwong M, Nair S, Pukrittayakamee S, Sudimack D, Williams JT, Mayxay M, Newton PN, Kim JR, Nandy A, Osorio L, Carlton JM, White NJ, Day NP, Anderson TJ. Contrasting genetic structure in Plasmodium vivax populations from Asia and South America. Int J Parasitol 2007; 37: 1013-1022. https://doi.org/10.1016/j.ijpara.2007.02.010
  63. Karunaweera ND, Ferreira MU, Hartl DL, Wirth DF. Fourteen polymorphic microsatellite DNA markers for the human malaria parasite Plasmodium vivax. Mol Ecol Notes 2007; 7: 172-175.
  64. Orjuela-Sanchez P, da Silva NS, da Silva-Nunes M, Ferreira MU. Recurrent parasitaemias and population dynamics of Plasmodium vivax polymorphisms in rural Amazonia. Am J Trop Med Hyg 2009; 81: 961-968. https://doi.org/10.4269/ajtmh.2009.09-0337
  65. Auburn S, Barry AE. Dissecting malaria biology and epidemiology using population genetics and genomics. Int J Parasitol 2017; 47: 77-85. https://doi.org/10.1016/j.ijpara.2016.08.006
  66. Putaporntip C, Jongwutiwes S, Sakihama N, Ferreira MU, Kho WG, Kaneko Akira, Kanbara H, Hattori T, Tanabe K. Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface protein-1 locus. Proc Natl Acad Sci USA 2002; 99: 16348-16353. https://doi.org/10.1073/pnas.252348999
  67. Ree HI. Unstable vivax malaria in Korea. Korean J Parasitol 2000; 38: 119-138. https://doi.org/10.3347/kjp.2000.38.3.119
  68. World Health Organization. World Malarias Report 2010. Geneva, Switzerland. World Health Organization. 2010.
  69. Gunawardena S, Ferreira MU, Kapilananda GMG, Wirth DF, Karunaweera ND. The Sri Lankan paradox: high genetic diversity in Plasmodium vivax populations despite decreasing levels of malaria transmission. Parasitology 2014; 141: 880-890. https://doi.org/10.1017/S0031182013002278
  70. Abdullah NR, Barber BE, William T, Norahmad NA, Satsu UR, Muniandy PK, Ismail Z, Grigg MJ, Jelip J, Piera K, von Seidlein L, Yeo TW, Anstey NM, Price RN, Auburn S. Plasmodium vivax population structure and transmission dynamics in Sabah Malaysia. PLoS One 2013; 8: e82553. https://doi.org/10.1371/journal.pone.0082553
  71. Hamedi Y, Sharifi-Sarasiabli K, Dehghan F, Safari R, To S, Handayuni I, Trimarsanto H, Price RN, Auburn S. Molecular epidemiology of P. vivax in Iran: High diversity and complex substructure using neutral markers, but no evidence of Y976F mutation at PvMDR1. PLoS One 2016; 11: e0166124. https://doi.org/10.1371/journal.pone.0166124
  72. Chung JY, Chun EH, Chun JH, Kho WG. Analysis of the Plasmodium vivax apical membrane antigen-1 gene from re-emerging Korean isolatesd. Parasitol Res 2003; 90: 325-329. https://doi.org/10.1007/s00436-002-0777-2
  73. Kim T, Kim YJ, Song KJ, Song JW, Cha SH, Kim YK, Shin YK, Sih IB, Lim CS. The molecular characteristics of circumsporozoite protein gene subtypes from Plasmodium vivax isolates in the Republic of Korea. Parasitol Res 2002; 88: 1051-1054. https://doi.org/10.1007/s00436-002-0699-z
  74. Kho WK, Park YH, Chung JY, Kim JP, Hong ST, Lee WJ, Kim TS, Lee JS. Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea. Korean J Parasitol 1999; 37: 265-270. https://doi.org/10.3347/kjp.1999.37.4.265
  75. Kim SH, Hwang SY, Shin JW, Moon CS, Kim DW, Kho WK. Molecular genetic characterization of the merozoite surface protein 1 gene of Plasmodium vivax from reemerging Korean isolates. Clin Vaccine Immunol 2009; 16: 733-738. https://doi.org/10.1128/CVI.00493-08
  76. Han ET, Wang Y, Lim CS, Cho JH, Chai JY. Genetic diversity of the malaria vaccine candidate merozoite surface protein 1 gene of Plasmodium vivax field isolates in the Republic of Korea. Parasitol Res 2011; 109: 1571-1576. https://doi.org/10.1007/s00436-011-2413-5
  77. Kang JM, Ju HL, Kang YM, Lee DH, Moon SU, Sohn WM, Park JW, Kim TS, Na BK. Genetic polymorphism and natural selection in the C-terminal 42 kDa region of merozoite surface protein-1 among Plasmodium vivax Korean isolates. Malaria J 2012; 11: 206. https://doi.org/10.1186/1475-2875-11-206
  78. Son UH, Dinzouna-Boutamba SD, Lee S, Yun HS, Kim JY, Joo SY, Jeong S, Rhee MH, Hong Y, Chung DI, Kwak D, Goo YK. Diversity of Vir genes in Plasmodium vivax from endemic regions in the Republic of Korea: an initial evaluation. Korean J Parasitol 2017; 55: 149-158. https://doi.org/10.3347/kjp.2017.55.2.149
  79. Jang JW, Yun SG, Woo MK, Han ET, Lu F, Gao Q, Yoon SY, An SSA, Lim CS. Sequence polymorphisms of Plasmodium vivax tryptophan and alanine rich antigen (PvTARAg55). Acta Trop 2015; 142: 122-126. https://doi.org/10.1016/j.actatropica.2014.11.007
  80. Jang JW, Cho CH, Kim JY, Koh YE, Woo MK, Kim KA, Yoon SY, Lim MS, Han ET, AN SSA, Lim CS. Rapid genotyping of Plasmodium vivax pvs25 and Pv38 genes by using mismatch specific endonuclease. Trop Biomed 2014; 31: 600-606.
  81. Woo MK, Kim KA, Kim JY, Oh JS, Han ET, An SSA, Lim CS. Sequence polymorphisms in Pvs48/45 and Pvs47 gametocyte and gamete surface proteins in Plasmodium vivax isolated in Korea. Mem Inst Oswaldo Cruz 2013; 108: 359-367. https://doi.org/10.1590/S0074-02762013000300015
  82. Iwagami M, Hwang SY, Fukumoto M, Hayakawa T, Tanabe K, Kim SH, Kho WG, Kano S. Geographic origin of Plasmodium vivax in the Republic of Korea: haplotype network analysis based on the parasite’s mitochondrial genome. Malaria J 2010; 9: 184. https://doi.org/10.1186/1475-2875-9-184