대한수의학회지 (Korean Journal of Veterinary Research)

  • 제58권1호
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  • Pages.9-16
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  • 2018
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
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Preliminary assessment of correlation between T-lymphocyte responses and control of porcine reproductive and respiratory syndrome virus (PRRSV) in piglets born after in-utero infection of a type 2 PRRSV

  • Cha, Sang-Ho (Animal and Plant Quarantine Agency) ;
  • Bandaranayaka-Mudiyanselage, Carey (VMRD Inc.) ;
  • Bandaranayaka-Mudiyanselage, Chandima B. (VMRD Inc.) ;
  • Ajiththos, Dharani (Department of Veterinary Microbiology and Pathology, Washington State University) ;
  • Yoon, Kyoung-Jin (Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University) ;
  • Gibson, Kathleen A. (Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University) ;
  • Yu, Ji-Eun (Animal and Plant Quarantine Agency) ;
  • Cho, In-Soo (Animal and Plant Quarantine Agency) ;
  • Lee, Stephen S. (University of Idaho) ;
  • Chung, Chungwon J. (VMRD Inc.)
  • 투고 : 2017.11.11
  • 심사 : 2018.02.14
  • 발행 : 2018.03.31
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초록

A preliminary study into the protective mechanisms of adaptive immunity against porcine reproductive and respiratory syndrome virus (PRRSV) in piglets (n = 9) born to a gilt challenged intranasally with a type-2 PRRSV. Immune parameters (neutralizing antibodies, $CD3^+CD4^+$, $CD3^+CD8^+$, $CD3^+CD4^+CD8^+$ T-lymphocytes, and PRRSV-specific interferon $(IFN)-{\gamma}$ secreting T-lymphocytes) were compared with infection parameters (macro- and microscopic lung lesion, and PRRSV-infected porcine alveolar macrophages ($CD172{\alpha}^+PRRSV-N^+\;PAM$) as well as with plasma and lymphoid tissue viral loads. Percentages of three T-lymphocyte phenotypes in 14-days post-birth (dpb) peripheral blood mononuclear cell (PBMC) had significant negative correlations with percentages of $CD172{\alpha}^+PRRSV-N^+\;PAM$ (p < 0.05) as well as with macroscopic lung lesion (p < 0.01). Plasma and tissue viral loads had significant (p < 0.05) negative correlations with $CD3^+CD4^+CD8^+$ T-lymphocyte percentage in PBMC. Frequencies of $CD3^+CD8^+$ and $CD3^+CD4^+$ T-lymphocytes in 14-dpb PBMC had significant negative correlations with of lymph node (p = 0.04) and lung (p = 0.002) viral loads. $IFN-{\gamma}$-secreting T-lymphocytes frequency had a significant negative correlation with gross lung lesion severity (p = 0.002). However, neutralizing antibody titers had no significant negative correlation (p > 0.1) with infection parameters. The results indicate that T-lymphocytes contribute to controlling PRRSV replication in young piglets born after in-utero infection.

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참고문헌

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