Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Anti-Oxidative and Anti-Neuroinflammatory Effect of Ethanol Extracts from Walnuts's (Juglans regia L.) Shell

  • Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankook University)
  • Received : 2018.11.11
  • Accepted : 2018.12.05
  • Published : 2018.12.31
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Abstract

In this study, antioxidant and anti-neuroinflammatory of ethanol extracts from walnuts's (Juglans regia L.) shell were investigated in vitro. Radical-scavenging activities of the walnuts's shell ethanol extracts (WSE) were examined by using ABTS radicals and ${\alpha},{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) radicals assay. In the ABTS and DPPH radical scavenging activity, $RC_{50}$ of WSE were measured as 15.74 and $40.13{\mu}g/mL$, respectively. Also, to evaluate the anti-neuroinflammatory effects of WSE in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. The production of proinflammatory cytokines NO were examined by LPS in BV-2 cell. BV-2 cells activated with LPS were treated with various doses (10, 25, 50, $100{\mu}g/mL$) of WSE. Supernatants were analyzed for the production of NO using Griess reagent. WSE up to $10{\mu}g/mL$ still required to inhibit NO induced by LPS. These results showed that walnuts's (Juglans regia L.) shell can be used as an easily accessible source of natural anti-neuroinflammatory and natural antioxidants.

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References

  1. Akbari V, Jamei R, Heidari R, Esfahlan AJ. Antiradica lactivity of different parts of Walnut (Juglans regia L.) fruit as a function of genotype. Food Chemistry. 2012. 135: 2404-2410. https://doi.org/10.1016/j.foodchem.2012.07.030
  2. Ames BN, Saul RL. Oxidative DNA damage, cancer and aging. Oxygen and human disease. Annals of Internal Medicine. 1987. 107: 536-539.
  3. Boje KM, Arora PK. Microglial-produced nitric oxide and reactive nitrogen oxides mediate neuronal cell death. Brain Research. 1992. 587: 250-256. https://doi.org/10.1016/0006-8993(92)91004-X
  4. Folin O, Denis W. On phosphotungstic-phosphomolybdic compounds as color reagents. The Journal of Biological Chemistry. 1912. 12: 239-249.
  5. Green LC, Wagner DA, Glogowski J. Analysis of nitrate, and [15N]nitrate in biological fluids. Analytical Biochemistry. 1982. 126: 131-138. https://doi.org/10.1016/0003-2697(82)90118-X
  6. Grosso G, Galvano F, Marventano S, Malaguarnera M, Bucolo C, Drago F, Caraci F. Omega-3 Fatty Acids and Depression: Scientific Evidence and Biological Mechanisms. Oxidative Medicine and Cellular Longevity. 2014. doi: 10.1155/2014/ 313570.
  7. Han KL, Kim MR, Jo BK, Kim MJ, Kang MJ, Park KH, Koo YE, Kim BS, Jung EG, Han MD. Antimicrobial and antioxidative activities of the extracts from Walnut (Juglans regia L.) green Husk. Journal of Life Science. 2015. 25: 433-440. https://doi.org/10.5352/JLS.2015.25.4.433
  8. Hou YC, Janczuk A, Wang PG. Current trends in the development of nitric oxide donors. Current Pharmaceutical Design. 1999. 5: 417-441.
  9. Jeong CH, Choi SG, Heo HJ. Analysis of nutritional components and evaluation of functional activities of Sasaborealis leaf tea. Korean Journal of Food Science and Technology. 2008. 40: 586-592.
  10. Kim TS, Kang SJ, Park WC. Changes in antioxidants and antioxidants enzymes activities of soybean leaves subject to water stress. Journal of the Korean Society for Applied Biological Chemistry. 1999. 42: 246-251.
  11. Knott AB, Perkins G, Schwarzendacher R, Bossy-Wetzel E. Mitochondrial fragmentation in neurodegeneration. Nature Reviews Neuroscience. 2008. 9: 505-518. https://doi.org/10.1038/nrn2417
  12. Kumar R, Balaji S, Sripriya R, Nithya N, Uma TS, Sehgal PK. In vitro evaluation of antioxidants of fruit extract of Momordica charantia L. on fibroblasts and erantinocytes. Journal of Agricultural and Food Chemistry. 2010. 58: 1518-1522. https://doi.org/10.1021/jf9025986
  13. Lee HS, Park YW. Antioxidant activity and antibacterial activities from different parts of broccoli extracts under high temperature. Journal of the Korean Society of Food Science and Nutrition. 2005. 34: 759-764. https://doi.org/10.3746/jkfn.2005.34.6.759
  14. Liu F, Ng TB. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sciences. 2000. 66: 725-735. https://doi.org/10.1016/S0024-3205(99)00643-8
  15. Maqsood S, Benjakul S. Comparative studies of four different phenolic compounds on in vitro antioxidative activity and the preventive effect on lipid oxidation of fish oil emulsion and fish mince. Food Chemistry. 2010. 119: 123-132. https://doi.org/10.1016/j.foodchem.2009.06.004
  16. Nieva, Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology. 2000. 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999. 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  18. Reynolds A, Lauriea C, Mosleya LR, Gendelmana EH. Oxidative stress and the pathogenesis of neurodegenerative disorders. International Review of Neurobiology. 2007. 82: 297-325.
  19. Rosen D, Siddique T, Patterson D, Figlewiez D, Sapp P, Hentai A, Donaldson D, Goto J, O Regan J, Deng H, Rahmani Z, Krrizus A. Mutation in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis. Nature London. 1993. 362: 59-62. https://doi.org/10.1038/362059a0
  20. Valko MD, Leibfritz J, Moncol MT, Cronin M, Mazur, Telser J. Free radical and antioxidants in normal physiological function and human disease. The International Journal of Biochemistry & Cell Biology. 2007. 39: 44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  21. Zamora R, Vodovotz Y, Billiar TR. Inducible nitric oxide synthase and inflammatory diseases. Molecular Medicine. 2000. 6: 347-373. https://doi.org/10.1007/BF03401781