대한의생명과학회지 (Biomedical Science Letters)

  • 제24권4호
  • /
  • Pages.341-348
  • /
  • 2018
  • /
  • 1738-3226(pISSN)
  • /
  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지
DOI QR코드

DOI QR Code

Effects of Gyeongshingangjeehwan 18 on Pancreatic Fibroinflammation in High-Fat Diet-Fed Obese C57BL/6J Mice

  • Jang, Joonseong (Department of Biomedical Engineering, Mokwon University) ;
  • Park, Younghyun (Department of Biomedical Engineering, Mokwon University) ;
  • Yoon, Michung (Department of Biomedical Engineering, Mokwon University)
  • 투고 : 2018.09.18
  • 심사 : 2018.12.10
  • 발행 : 2018.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The polyherbal drug Gyeongshingangjeehwan 18 (GGEx18) from Rheum palmatum L. (Polygonaceae), Laminaria japonica Aresch (Laminariaceae), and Ephedra sinica Stapf (Ephedraceae) has traditionally been used as an antiobesity drug in Korean local clinics. This study investigates the effects of GGEx18 on pancreatic fibroinflammation in high-fat diet (HFD)-fed obese C57BL/6J mice and the molecular mechanism involved in this process. After HFD-fed obese C57BL/6J mice were treated with GGEx18 (125, 250, and 500 mg/kg) for 12 weeks, variables and determinants of obesity, pancreatic inflammation, and fibrosis were measured using histology, immunohistochemistry, and real-time polymerase chain reaction. Administration of GGEx18 at 500 mg/kg/day to obese mice decreased body weight gain, mesenteric adipose tissue mass, and adipocyte size. GGEx18 treatment not only reduced mast cells and CD68-immunoreactive cells, but also decreased collagen levels and ${\alpha}$-smooth muscle actin-positive cells in the pancreas of HFD-fed mice. Concomitantly, GGEx18 decreased the expression of genes for inflammation (i.e., CD68 and tumor necrosis factor ${\alpha}$) and fibrosis (i.e., collagen ${\alpha}1$ and transforming growth factor ${\beta}$) in the pancreas of obese mice. These results suggest that GGEx18 may inhibit visceral obesity and related pancreatic fibroinflammation in HFD-fed obese mice.

키워드

참고문헌

  1. Acharya C, Navina S, Singh VP. Role of pancreatic fat in the outcomes of pancreatitis. Pancreatology. 2014. 14: 403-408. https://doi.org/10.1016/j.pan.2014.06.004
  2. Cao Y, Bao S, Yang W, Zhang J, Li L, Shan Z, Teng W. Epigallocatechin gallate prevents inflammation by reducing macrophage infiltration and inhibiting tumor necrosis factor-$\alpha$ signaling in the pancreas of rats on a high-fat diet. Nutrition Research. 2014. 34: 1066-1074. https://doi.org/10.1016/j.nutres.2014.10.004
  3. Chen KK. A comparative study of synthetic and natural ephedrines. The Journal of Pharmacology and Experimental Therapeutics. 1928. 33: 237-258.
  4. Duan LF, Xu XF, Zhu LJ, Liu F, Zhang XQ, Wu N, Fan JW, Xin JQ, Zhang H. Dachaihu decoction ameliorates pancreatic fibrosis by inhibiting macrophage infiltration in chronic pancreatitis. World Journal of Gastroenterology. 2017. 23: 7242-7252. https://doi.org/10.3748/wjg.v23.i40.7242
  5. Fernandes-Santos C, Evangelista Carneiro R, de Souza Mendonca L, Barbosa Aguila M, Mandarim-de-Lacerda CA. Rosiglitazone aggravates nonalcoholic Fatty pancreatic disease in C57BL/6 mice fed high-fat and high-sucrose diet. Pancreas. 2009. 38: e80-e86. https://doi.org/10.1097/MPA.0b013e3181987d9d
  6. Greenway FL, De Jonge L, Blanchard D, Frisard M, Smith SR. Effect of a dietary herbal supplement containing caffeine and ephedra on weight, metabolic rate, and body composition. Obesity Research. 2004. 12: 1152-1157. https://doi.org/10.1038/oby.2004.144
  7. Holness CL, Simmons DL. Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood. 1993.81: 1607-1613.
  8. Lee H, Lim J, Park Y, Jang J, Yoon S, Ahn J, Nam MH, Shin SS, Yoon M. Gyeongshingangjeehwan 18, a polyherbal composition, deters glucose intolerance and nonalcoholic fatty pancreatic disease in C57BL/6J mice on a high-fat diet. Journal of Ethnopharmacology. (Submitted).
  9. Lim J, Lee H, Ahn J, Kim J, Jang J, Park Y, Jeong B, Yang H, Shin SS, Yoon M. The polyherbal drug GGEx18 from Laminaria japonica, Rheum palmatum, and Ephedra sinica inhibits hepatic steatosis and fibroinflammtion in high-fat diet-induced obese mice. Journal of Ethnopharmacology. 2018. 225: 31-41. https://doi.org/10.1016/j.jep.2018.06.034
  10. Mathur A, Marine M, Lu D, Swartz-Basile DA, Saxena R, Zyromski NJ, Pitt HA. Nonalcoholic fatty pancreas disease. HPB (Oxford). 2007. 9: 312-318. https://doi.org/10.1080/13651820701504157
  11. Noel P, Patel K, Durgampudi C, Trivedi RN, de Oliveira C, Crowell MD, Pannala R, Lee K, Brand R, Chennat J, Slivka A, Papachristou GI, Khalid A, Whitcomb DC, DeLany JP, Cline RA, Acharya C, Jaligama D, Murad FM, Yadav D, Navina S, Singh VP. Peripancreatic fat necrosis worsens acute pancreatitis independent of pancreatic necrosis via unsaturated fatty acids increased in human pancreatic necrosis collections. Gut. 2016. 65: 100-111. https://doi.org/10.1136/gutjnl-2014-308043
  12. Okuno A, Tamemoto H, Tobe K, Ueki K, Mori Y, Iwamoto K, Umesono K, Akanuma Y, Fujiwara T, Horikoshi H, Yazaki Y, Kadowaki T. Troglitazone increases the number of small adipocytes without the change of white adipose tissue mass in obese Zucker rats. The Journal of Clinical Investigation. 1998. 101: 1354-1361. https://doi.org/10.1172/JCI1235
  13. Smith U. Abdominal obesity: a marker of ectopic fat accumulation. The Journal of Clinical Investigation. 2015. 125: 1790-1792. https://doi.org/10.1172/JCI81507
  14. van Herpen NA, Schrauwen-Hinderling VB. Lipid accumulation in non-adipose tissue and lipotoxicity. Physiol Behav. 2008. 94: 231-241. https://doi.org/10.1016/j.physbeh.2007.11.049
  15. Wang J, Zhao Y, Xiao X, Li H, Zhao H, Zhang P, Jin C. Assessment of the renal protection and hepatotoxicity of rhubarb extract in rats. Journal of Ethnopharmacology. 2009. 124: 18-25. https://doi.org/10.1016/j.jep.2009.04.018
  16. Yang M, Li X, Zeng X, Ou Z, Xue M, Gao D, Liu S, Li X, Yang S. Rheum palmatum L. attenuates high fat diet-induced hepatosteatosis by activating AMP-activated protein kinase. The American Journal of Chinese Medicine. 2016. 44: 551-564. https://doi.org/10.1142/S0192415X16500300
  17. Yen HJ. Laminaria japonica Aresch. In: Chinese Pharmaceutics of Maine Lakes and Marshes. Xueyuan Press, Beijing, China. 1996.
  18. Yoon,KH, Lee HY, Jung YS, Seo BI, Park KY, Yoon M, Shin SS. Modulation of obesity by Gyeongshingangjeehwan 18 in ob/ob mice. Koran Journal of Hoerbology. 2010. 25: 1-9.
  19. Zhang X, Cui Y, Fang L, Li F. Chronic high-fat diets induce oxide injuries and fibrogenesis of pancreatic cells in rats. Pancreas. 2008. 37: e31-e38. https://doi.org/10.1097/MPA.0b013e3181744b50