Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Inhibitory Effects of Carex pumila Extracts on MMP-2 and MMP-9 Activities in HT-1080 Cells

HT-1080 세포주에서 좀보리사초 추출물의 MMP-2와 MMP-9 활성 억제효과

  • Kim, Junse (Ocean Science and Technology School, Korea Maritime and Ocean University) ;
  • Kong, Chang-Suk (Department of Food and Nutrition, College of Medical & Life Sciences, Silla University) ;
  • Seo, Youngwan (Ocean Science and Technology School, Korea Maritime and Ocean University)
  • 김준세 (한국해양대학교 해양과학기술전문대학원) ;
  • 공창숙 (신라대학교 식품영양학과) ;
  • 서영완 (한국해양대학교 해양과학기술전문대학원)
  • Received : 2018.11.06
  • Accepted : 2018.12.19
  • Published : 2018.12.30
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Abstract

Matrix metalloproteinases (MMPs) are associated with the invasion and metastasis of malignant tumors composed of cancer cells in an increased state of expression. This study evaluates the inhibitory effect of Carex pumila on MMP-2 and MMP-9 activity in phorbol-12-myristate-13-acetate (PMA)-stimulated HT-1080 human fibrosarcoma cells using gelatin zymography, MMPs enzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assay. C. pumila was extracted twice with dichloromethane ($CH_2Cl_2$) and methanol (MeOH). Treatment with $CH_2Cl_2$ extract and MeOH extract in PMA-stimulated HT-1080 cells effectively reduced the production of MMP-2 and 9. Also, the combined crude extracts ($CH_2Cl_2$ and MeOH) significantly inhibited the enzymatic activities and the expression of MMP-2 and MMP-9 in mRNA and protein levels. The combined crude extracts were partitioned between $CH_2Cl_2$ and water. The organic layer was further fractionated with n-hexane, 85% aqueous methanol (85% aq.MeOH) and the aqueous layer was separated into n-butanol and water, successively. Of the fractions, 85% aq.MeOH fraction showed the highest inhibitory activity of MMP-2 and MMP-9 in gelatin zymography and MMP ELISA kit. Furthermore, 85% aq.MeOH fraction most significantly suppressed cell migration. In RT-PCR and Western blot assay, n-butanol and 85% aq.MeOH fractions exerted the greatest inhibition on mRNA and protein expression of MMP-2 and MMP-9, respectively. As a result, C. pumila can be used as a good anti-invasive agent source.

Keywords

References

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