생약학회지 (Korean Journal of Pharmacognosy)

  • 제49권4호
  • /
  • Pages.349-361
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  • 2018
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

소청룡탕 제조방법에 따른 성분 및 생리활성 비교

Comparison of Marker Components and Biological Activities of Socheongryong-tang by Different Extract Methods

  • Baek, Ka Yeon (National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Se Jin (National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Jong Beom (National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Jeong Hyeon (National Development Institute of Korean Medicine (NIKOM)) ;
  • Moon, Sung Ok (National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Hwa Dong (National Development Institute of Korean Medicine (NIKOM))
  • 투고 : 2018.11.28
  • 심사 : 2018.12.17
  • 발행 : 2018.12.31
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초록

Socheongryong-tang (SCRT) was one of the major traditional herbal medicines wildly used in the treatment of respiratory disease. SCRT is being commercially produced in the form of mix extracts powder and soft dry extract by different extract methods in the Korean Herbal Pharmacopeia (KHP). In this study, the contents of marker components and biological activities of the SCRT mix extract powder were compared with those of the SCRT decoction. To analyze the marker components of SCRT, nine marker from eight herbal preparations were chosen. And the method using high performance liquid chromatography (HPLC) with diode-array detector method was established for the simultaneous analysis. Method validation was accomplished by linearity, precision test, and recovery test. The contents of nine marker components in this extract was ascertained by ratio. The biological activities were examined the effect of SCRT on anti-oxidation and pro-inflammation mediated by LPS-stimulation. We confirmed that both of SCRT mix extrct powder and decoction have the similar contents on total polyphenol and flavonoid and inhibited the secretion of nitric oxide (NO), $IL-1{\beta}$, IL-6, tumor necrosis factor $(TNF)-{\alpha}$ and the expression of iNOS, COX-2, $IL-1{\beta}$, IL-6, $TNF-{\alpha}$. These results suggest that SCRT mix extract powder and decoction have a significant correlation.

키워드

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