Journal of the Korea Organic Resources Recycling Association (유기물자원화)

Korea Organic Resources Recycling Association (유기성자원학회)
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Recovery and Characterization of Lactic Acid from Fermentation Broth Using Chemical Precipitation

화학침전을 이용한 발효액의 젖산 회수 및 유기물 특성분석

  • Lee, Wontae (Department of Environmental Engineering, Kumoh National Institute of Technology)
  • 이원태 (금오공과대학교 환경공학과)
  • Received : 2018.03.21
  • Accepted : 2018.03.27
  • Published : 2018.03.30
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Abstract

Recovery of lactic acid from fermentation broth using chemical precipitation was investigated with various chemicals. Effects of chemical types, mixing speeds, settling duration, and solvent addition were evaluated to improve the recovery rates of lactic acid. Overall, recovery efficiencies increased as the dosage of chemicals increased. Recovery rate of lactic acid by CaO was higher than those of $Ca(OH)_2$ and $CaCO_3$. Recovery of lactic acid increased by 48% under the optimized reaction conditions which included a mixing speed at 180 rpm, a settling duration of 24 h, and addition of ethanol at 25%(v/v). Practical application needs to consider types and concentrations of other organic acids as well as lactic acid. Based upon the results of fluorescence excitation emission matrix (FEEM), size exclusion chromatography (SEC), characteristics of recovered lactic acid were same as that in the fermentation broth.

유기성 폐기물 소화공정의 발효액에서 젖산(lactic acid)을 회수하기 위해 화학침전법을 평가하였다. 젖산(lactic acid)의 회수율을 높이기 위하여 화학침전제 종류와 교반속도 및 침전시간 등 반응조건이 회수율 향상에 미치는 영향을 살펴보았다. 화학침전제의 종류에 관계없이 주입양이 증가할수록 젖산(lactic acid) 회수율이 증가하는 경향을 보였으며, CaO가 $Ca(OH)_2$와 $CaCO_3$에 비하여 높은 회수율을 나타냈다. CaO를 사용한 반응조건 최적화 실험결과, 교반속도 180 rpm, 침전시간 24 h, ethanol 주입량 25%(v/v)에서 회수율이 48%로 가장 높게 나타났다. 본 연구에서는 발효액 내 젖산(lactic acid)의 농도만 고려하여 주입할 침전제의 양을 계산하였기 때문에 실제 적용을 위해서는 발효액의 유기산 종류 및 농도를 고려하여 침전제의 투입량을 결정해야할 것이다. 유기물의 정성적 분석(FEEM, SEC) 결과로 볼 때 침전공정은 유기산의 특성에 큰 영향을 미치지 않았다.

Keywords

References

  1. [한밭대학교, "유기성 폐기물의 자원화 극대화를 위한 고효율 통합 패키지 공정 개발"], Hanbat University, "Development of high-rate integrated package processes for the maximization of energy recovery from organic waste". (2013).
  2. Martinez, F.A.C., Balciunas, E.M., Salgado, J.M.,"Lactic acid properties, applications and production: A review", Trends in Food Science and Technology, 30(1), pp. 70-83. (2012). https://doi.org/10.1016/j.tifs.2012.11.007
  3. Rogers, P., Chen, J.S., Zidwick, M.J., "Organic acid and solvent production : acetic, lactic, gluconic, succinic, and polyhydroxyalkanoic acids", Springer-Verlag, Dusseldorf, Germany, pp. 511-755. (2006).
  4. Sauer, M., Porro, D., Mattanovich, D., Branduardi, P., "Microbial production of organic acids: expanding the markets:, Trends in Biotechnology, 26(2), pp. 100-108. (2008). https://doi.org/10.1016/j.tibtech.2007.11.006
  5. [우대식, 조영민, 김보경, 황휘동, 한명완, "분리벽형 반응증류탑을 이용한 젖산회수", 화학공학 회지] Woo, D.S, Cho, Y.M., Kim, B.K., Hwang, H.D., Han, M.W., "Recovery of Lactic Acid Using Reactive Dividing Wall Column", Korean Chemical Engineering Research, 48(3), pp. 342-349. (2009).
  6. Pal, P., Sikder, J., Roy, S., Giorno, L, "Process intensification in lactic acid production: A review of membrane based processes", Chemical Engineering and Processing: Process Intensification, 48(11-12), pp. 1549-1559. (2009). https://doi.org/10.1016/j.cep.2009.09.003
  7. Dey, P., Linnanen, L., Pal, P.,"Separation of lactic acid from fermentation broth by cross flow nanofiltration: Membrane characterization and transport modelling", Desalination, 288, pp. 47-57. (2012). https://doi.org/10.1016/j.desal.2011.12.009
  8. Song, M., Jiao, P., Qin, T., Jiang, K., Zhou, J., Zhuang, W., Chen, Y., Liu, D., Zhu, C., Chen, X., Ying, H. Wu, J., "Recovery of lactic acid from the pretreated fermentation broth based on a novel hyper-cross-linked meso-micropore resin: Modeling", Bioresource Technology, 241, pp. 593-602. (2017). https://doi.org/10.1016/j.biortech.2017.05.179
  9. Taleghani, H.G., Ghoreyshi, A.A., Najafpour, G.D., "Thin film composite nanofiltration membrane for lactic acid production in membrane bioreactor", Biochemical Engineering Journal, 132, pp. 152-160. (2018). https://doi.org/10.1016/j.bej.2018.01.020
  10. [최국화, 장용근, 김진현, "Lactic acid 회수를 위한 침전공정 최적화", KSBB Journal] Choi, K.H., Chang, Y.K., Kim, J.H., "Optimization of Precipitation Process for the Recovery of Lactic Acid", Korean Society for Biotechnology and Bioengineering Journal, 26(1), pp. 13-18. (2011).
  11. Kim, D., Lee, M., Hwang, Y., Im, W., Yun, Y., Park, C., Kim, M., "Microbial granulation for lactic acid production", Biotechnology and Bioengineering, 113, pp. 101-111. (2016). https://doi.org/10.1002/bit.25540
  12. Jeon, S.I., Mun, S., Kim, J.H., "Optimal temperature control in fractional precipitation for paclitaxel pre-purification", Process Biochemistry, 41(2), pp. 276-280. (2006). https://doi.org/10.1016/j.procbio.2005.07.016
  13. [박석찬, 이상목, 김영준, 김우식, 구윤모, "발효액으로부터 침전과 반응증류를 이용한 젖산의 회수", 한국생물공학회지] Park, S.C., Lee, S.M., Kim, Y.J., Kim, W.S., Koo, Y.M., "Recovery of lactic acid from fermentation broth using precipitation and reactive distillation". Korean Journal of Biotechnology and Bioengineering., 21(3), pp. 199-203. (2006).