The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects for the New Formulation of Daesiho-tang on adipocyte development and differentiation in 3T3-L1

대시호탕의 새로운 제형이 3T3-L1에서 지방세포 증식과 분화 과정에 미치는 영향

  • Choi, Hye-Min (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Se-Jin (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Moon, Sung-Ok (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Ji-Beom (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Ha-young (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Jong-Beom (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Hwa-Dong (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM))
  • 최혜민 (한약진흥재단, 한약제제팀) ;
  • 김세진 (한약진흥재단, 한약제제팀) ;
  • 문성옥 (한약진흥재단, 한약제제팀) ;
  • 이지범 (한약진흥재단, 한약제제팀) ;
  • 이하영 (한약진흥재단, 한약제제팀) ;
  • 김종범 (한약진흥재단, 한약제제팀) ;
  • 이화동 (한약진흥재단, 한약제제팀)
  • Received : 2018.01.18
  • Accepted : 2018.03.15
  • Published : 2018.03.30
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Abstract

Objectives : Daesiho-tang (DSHT) has been widely used in the treatment of cerebral infarct in traditional medicine. However, there was not report on the anti-obesity-related diseases efficacy of DSHT. In this study, we investigated the effects for the new formulation of DSHT, on the adipocyte differentiation cycle in 3T3-L1 cells. Methods : 3T3-L1 cells were treated with DSHT (50, 100, $200{\mu}g/m{\ell}$) during differentiation for 6 days. Also, the inhibitory effect of DSHT against 3T3-L1 adipogenesis was evaluated in various stage of adipogenesis such as early (0-2day), intermediate (2-4day), and terminal stage (4-6day). The accumulation of lipid droplets was determined by Oil Red O staining. and, the expressions of genes related to adipogenesis were measured by RT-PCR and Western blot analyses. Results : DSHT showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affect cell toxicity as assessed by measuring fat accumulation and adipogenesis. In addition, DSHT significantly reduced the expression levels of several adipocyte marker genes including proliferator activated $receptor-{\gamma}$ ($PPAR-{\gamma}$) and CCAAT/ enhancer-binding $protein-{\alpha}$ ($C/EBP-{\alpha}$). Also, the anti-adipogenic effect of DSHT was strongly limited in the intermediate (2-4 day), terminal stage (4-6 day) of 3T3-L1 adipogenesis. In addition, the DSHT treatment down- regulated mRNA expression levels of $PPAR-{\gamma}$,, $C/EBP-{\alpha}$ in mature 3T3-L1 adipocytes. Conclusions : These results suggest that, the ability of DSHT has inhibited overall adipogenesis and lipid accumulation in the 3T3-L1 cells. The new formulation of DSHT may be a promising medicine for the treatment of obesity and related metabolic disorders.

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References

  1. Chae IS. Sanghanronyeukjun. Seoul: Gomundang. 2000:86-130.
  2. Hur J. Donguibogam. 3rd rev. ed. Seoul: Yeougang. 2005:745-1450.
  3. Kim Y, So HS, Kim SJ, Youn MJ, Lee JH, Kim NS, Lee JH, Woo WH, Lee DW, Cho KH, Moon BS, Park R. Antiinflammatory effect of Daesiho, a Korean traditional prescription for cerebral infarct patients. Phytother Res. 2008;22(6):829-35. https://doi.org/10.1002/ptr.2389
  4. Kim CH, Kweon YM, Lee YT, Park SD. The preventive effect of Daeshiho-tang on liver damageinduced by acetaminophen in the rats. Korean J Oriental Med Prescription. 2004; 12(2):139-154.
  5. Rha YG, Choi SH, Ahn KS, Moon JJ Preventive and Therapeutic Effect of Daeshihotang and Its Components on the Gallstone Formation of Mouse. Korean J Orient Physiol Patho. 1991;6:1-25.
  6. Jung H, Kang JC. Effect of Daesihotangsosonggitang-gagambang on liver injury of rats induced by ccl4 and d-galactosamine. Korean J. Orient. Int Med. 2000;21(2):267-274.
  7. Yeo UH, Jo HJ, Kim HH. Effects of DaeSiHo-Tang extract on Hypertension and Arterial Contraction. Korean J Orient Physiol Patho. 2005;19(6):1573-1579.
  8. Park CS, Park CK, Han SD, Park SD. A Experimental Study on the Effect of Kami-Daesihotang on Hyper1ipidemia & Hypertension. Korean J. Orient. Int Med. 1998;19(2):159-184.
  9. Lee YJ, Ji GU, Eom HS. An Experimental Study on the Effects of Dachaihutang he wulingsan on Hypertension and Hyperlipidemia. Korean J Orient Physiol Patho. 2000;14(2):48-69.
  10. Chun JM, Kim DS, Choi G, Sung YY, Park SH, Kim SH, Kim HK. Anti-obesity Effect of Five Formulas Which can be used to Treat Hyper-tension in High Fat Diet Induced Obese Mice. Korean J Orient Physiol Patho. 2013;27(5):637-643.
  11. Song HY, Kim EK, Kong JC, Lee JH, Shin BC, Ryu DG, Kwon KB. Inhibitory Effect of Daesiho-tang (Dachaihu-tang) Extracts on High-fat Diet-induced Obesity. J Oriental Rehab Med. 2008;18(3):41-49.
  12. Park, SD, Yun BG. ffects of Daesihotang and its component groups on diabetes, free radical and antioxidative defense system in alloxan induced diabetic rats. Korean J Orient Med Prescr. 2001; 9(1):289-317.
  13. Han MG, Seo HG, Kim EH. The Effects of Daesihotang on Body weight and Biochemical changes of rats fed high diet. Korean J Chuna manual Med. 2001;2(1):177-186.
  14. Korea Centers for Disease Control and Prevention. 2009 Korea national health and nutrition and examination survey. Ministry of Health and Welfare. 2010.
  15. Kopelman PG. Obesity as a medical problem. Nature. 2000;404:635-643. https://doi.org/10.1038/35007508
  16. Otto TC, Lane MD. Adipose development : from stem cell to adipocyte. Rev. Biochem. Mol. Biol. 2005;40:229-242. https://doi.org/10.1080/10409230591008189
  17. Lane MD, Tang QQ, Jiang MS. Role of the CCAAT enhancer binding proteins (C/EBPs) in adipocyte differentation. Biochem. Biophys. Res. Commun. 1999;266:677-683. https://doi.org/10.1006/bbrc.1999.1885
  18. Farmer SR. Transcriptional control of adipocyte formation. Cell Metab. 2006;4:263-273. https://doi.org/10.1016/j.cmet.2006.07.001
  19. Lin FT, Lane MD. CCAAT/enhancer binding protein alpha is sufficient to initiate the 3T3-L1 adipocyte differentiation program. Proc. Natl. Acad. Sci. USA. 1994;91:8757-8761. https://doi.org/10.1073/pnas.91.19.8757
  20. Rosen ED, Hsu CH, Wang X, Sakai S, Freeman MW, Conzales FJ, Spiegelma BM. C/EBP${\alpha}$ induces adipogenesis through PPAR$\gamma$: Aunified pathway. Genes Dev. 2002;16(1):22-26. https://doi.org/10.1101/gad.948702
  21. Camp HS, Ren D, Leff T. Adipogenesis and fatcell function in obesity and diabetes. Trends Mol Med. 2002;8(9):442-447. https://doi.org/10.1016/S1471-4914(02)02396-1
  22. Tamori Y, Masugi J, Nishino N, Kasuga M. Role of peroxisome proliferator activated receptor-$\gamma$ in maintenance of mature 3T3-L1 adipocytes. Diabetes. 2002;51(7):2045-2055. https://doi.org/10.2337/diabetes.51.7.2045
  23. Tanaka T, Itoh H, Doi K, Fukunaga Y, Hosoda K, Shintani M, Yamashita J, Chun TH, Inoue M, Masatsugu K, Sawada N, Saito T, Inoue G, Nishimura H, Yoshimasa Y, Nakao K. Down regulation of peroxisome proliferator activated receptor-$\gamma$ expression by inflamma- tory cytokines and its reversal by thiazolidine- dione. Diabetologia. 1999; 42(6):702-7. https://doi.org/10.1007/s001250051218