Effects for the New Formulation of Daesiho-tang on adipocyte development and differentiation in 3T3-L1

대시호탕의 새로운 제형이 3T3-L1에서 지방세포 증식과 분화 과정에 미치는 영향

  • Choi, Hye-Min (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Se-Jin (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Moon, Sung-Ok (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Ji-Beom (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Ha-young (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Jong-Beom (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Hwa-Dong (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM))
  • 최혜민 (한약진흥재단, 한약제제팀) ;
  • 김세진 (한약진흥재단, 한약제제팀) ;
  • 문성옥 (한약진흥재단, 한약제제팀) ;
  • 이지범 (한약진흥재단, 한약제제팀) ;
  • 이하영 (한약진흥재단, 한약제제팀) ;
  • 김종범 (한약진흥재단, 한약제제팀) ;
  • 이화동 (한약진흥재단, 한약제제팀)
  • Received : 2018.01.18
  • Accepted : 2018.03.15
  • Published : 2018.03.30


Objectives : Daesiho-tang (DSHT) has been widely used in the treatment of cerebral infarct in traditional medicine. However, there was not report on the anti-obesity-related diseases efficacy of DSHT. In this study, we investigated the effects for the new formulation of DSHT, on the adipocyte differentiation cycle in 3T3-L1 cells. Methods : 3T3-L1 cells were treated with DSHT (50, 100, $200{\mu}g/m{\ell}$) during differentiation for 6 days. Also, the inhibitory effect of DSHT against 3T3-L1 adipogenesis was evaluated in various stage of adipogenesis such as early (0-2day), intermediate (2-4day), and terminal stage (4-6day). The accumulation of lipid droplets was determined by Oil Red O staining. and, the expressions of genes related to adipogenesis were measured by RT-PCR and Western blot analyses. Results : DSHT showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affect cell toxicity as assessed by measuring fat accumulation and adipogenesis. In addition, DSHT significantly reduced the expression levels of several adipocyte marker genes including proliferator activated $receptor-{\gamma}$ ($PPAR-{\gamma}$) and CCAAT/ enhancer-binding $protein-{\alpha}$ ($C/EBP-{\alpha}$). Also, the anti-adipogenic effect of DSHT was strongly limited in the intermediate (2-4 day), terminal stage (4-6 day) of 3T3-L1 adipogenesis. In addition, the DSHT treatment down- regulated mRNA expression levels of $PPAR-{\gamma}$,, $C/EBP-{\alpha}$ in mature 3T3-L1 adipocytes. Conclusions : These results suggest that, the ability of DSHT has inhibited overall adipogenesis and lipid accumulation in the 3T3-L1 cells. The new formulation of DSHT may be a promising medicine for the treatment of obesity and related metabolic disorders.



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