The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Research for Reduction of Body Fat and Development of High Functionality in Young Persimmon Fruit

체지방 감소 고기능성 발현 떫은 감 소재 개발 연구

  • Kim, Soo Hyun (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Ah Reum (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Kyeong Jo (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Il Gyu (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Rhee, Man Hee (Laboratory of Veterinary Physiology & Cell Signaling, College of VeterinaryMedicine, Kyungpook National University) ;
  • Kim, Hyun-Kyoung (Department of Food Science and Engineering, Seowon University) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이아름 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김경조 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김일규 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이만휘 (경북대학교 수의과대학 생리학교실) ;
  • 김현경 (서원대학교 BIT 융합대학 식품공학과) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2018.02.20
  • Accepted : 2018.03.15
  • Published : 2018.03.30
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Abstract

Objectives : This study was conducted to evaluate the antioxidant activity and inhibitory activity of pancreatic lipase in vitro using natural products sangju dungsi, chungdo bansi, kabju baekmok three kind of young persimmon fruit extracts and to develop a high - functional anti - obesity substance with reduced body fat. Methods : The antioxidative effect of the three kind of young persimmon fruit extracts was determined by hot water extraction, 30% EtOH extraction and 70% EtOH extraction. The antioxidant activities were evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. Furthermore, we examined total phenol and flavonoids contents. And the pancreatic lipase inhibition was measured by selecting the sangju dungsi extract with antioxidant effect. Results : The yield of water, 30% and 70% EtOH extract from young persimmon fruit was sangju dungsi (water 8.3%, 30% EtOH 18.1%, 70% EtOH 16.9%), chungdo bansi (water 16.2%, 30% EtOH 16.1%, 70% EtOH 11.6%), kabju baekmok (water 16%, 30% EtOH 16.8%, 70% EtOH 11.5%). The sangju dungsi 30% EtOH extract eminent DPPH ($IC_{50}$, $19.25{\pm}2.94{\mu}g/m{\ell}$) and ABTS ($IC_{50}$, $19.94{\pm}4.07{\mu}g/m{\ell}$) radical scavenging activity, and total phenol $52.37{\pm}0.78mg/g$ and flavonoid $7.97{\pm}0.13mg/g$ contents were also excellent. It also showed excellent pancreatic lipase inhibition efficacy. Conclusion : The extracts from the three kind of young persimmon fruit extracts showed excellent antioxidant activity. sangju dungsi 30% EtOH extract may be a new anti-obesity material by reducing fat absorption through the inhibition of pancreatic lipase.

Keywords

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