Fig. 1. Ginseng hairy root cultures (A; solid culture and B; liquid culture) transformed by Agrobacterium rhizogene. Hairy roots grew at 25℃, pH 5.8, 3%(w/v) sucrose, and 1/2 strength of MS medium for 5 weeks.
Fig. 2. Ginseng hairy root cultures transformed by Agrobacterium rhizogene. Hairy roots grew at 25℃, pH 5.8, 2%(w/v) sucrose for 5 weeks. MS concentrations were 1/4, 1/2, 1, 2, and 3 strength of MS.
Fig. 3. Ginseng hairy root cultures transformed by Agrobacterium rhizogene. Hairy roots grew at 25℃, pH 5.8, 1/2 strength of MS for 5 weeks. The sucrose concentrations were 1, 2, 3, 5, and 10% (w/v).
Fig. 4. The TLC chromatogram and analysis of ginsenoside produced from root culture. HRP and HRF represent the ginseng hairy root cultures grown at Petri-dish solid and flask liquid culture.
Table 1. Yield and productivity of ginseng hairy root cultures transformed by Agrobacterium rhizogene. Hairy roots grew at 25℃ and pH 5.8 for 5 weeks. MS concentrations were 1/4, 1/2, 1, 2, and 3 strengrt of MS. The sucrose concentrations were 1, 2, 3, 5, and 10% (w/v)
Table 2. The amount of ginsenosides of ginseng hairy root cultures transformed by Agrobacterium rhizogene at the optimized conditions for 5 weeks
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