Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Analysis of Scutellaria baicaleinsis Georgi (Scutellariae Radix) by LC-DAD and LC-ESI/MS

  • Yu, Youngbob (Department of Herbal Pharmaceutical Development & Department of Emergency Medical Rescue, Nambu University) ;
  • Choi, Pil-Son (Department of Herbal Pharmaceutical Development & Department of Emergency Medical Rescue, Nambu University) ;
  • Koo, Sungtae (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Chang, Suhwan (Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center)
  • Received : 2018.11.22
  • Accepted : 2018.12.18
  • Published : 2018.12.31
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Abstract

In this study, baicalin, as a marker substance of Scutellariae Radix, was quantitatively analyzed by a high performance liquid chromatography-photodiode array detector (HPLC-DAD). We identified wogonoside, baicalein, and wogonin in the Scutellariae Radix by a high performance liquid chromatography-electrospray ionization-mass spectrometer (HPLC-ESI-MS). The baicalin was separated on a Xterra C18 column ($5{\mu}m$, $4.6{\times}250mm$) using mobile phase consisting of 38% acetonitrile in 0.68% phosphoric acid. The baicalin spectrum in the Scutellariae Radix extracts was coincided by comparing with UV-visible spectrum (200-550 nm) of baicalin standard in the library. The amount of baicalin in Scutellariae Radix was 10.46%, which is higher than KFDA's guideline. The marker substances of Scutellariae Radix showed a strong base peak $[M]^+$ in the positive detection mode following as; baicalin (m/z; $271[MH^+-sugar]^+$, $447[M+H]^+$), wogonoside (m/z; $285[MH^+-sugar]^+$, $461[M+H]^+$), baicalein (m/z; $271\;[M+H]^+$), wogonin (m/z; $285[M+H]^+$). These results are consistent with the fragment pattern and molecular weight of standard components from literature.

Keywords

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Fig. 1. HPLC chromatogram of Baicalin standard at 277 nm.

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Fig. 2. HPLC chromatogram of Scutellariae Radix extracts at 277 nm.

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Fig. 3. Spectral match plot of maker substance from Scutellariae Radix compared with baicalin standard (200-550 nm).

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Fig. 4. EIC (Extract ion current) chromatogram and mass spectra of baicalin (A, a), wogonoside (B, b), wogonin (C, c), baicalein (D,d) from Scutellariae Radix.

Table 1. Inter-day and intra-day relative standard deviations of baicalin (reproducibility) (n=3)

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Table 2. Relative recovery test of baicalin (n=3)

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