한국가금학회지 (Korean Journal of Poultry Science)

  • 제45권4호
  • /
  • Pages.253-260
  • /
  • 2018
  • /
  • 1225-6625(pISSN)
  • /
  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지
DOI QR코드

DOI QR Code

한국 재래계의 HNF4α 유전자 내 SNP와 성장과의 연관성 분석

Association of SNPs in the HNF4α Gene with Growth Performance of Korean Native Chickens

  • 양송이 (강원대학교 동물생명과학대학) ;
  • 최소영 (강원대학교 동물생명과학대학) ;
  • 홍민욱 (강원대학교 동물생명과학대학) ;
  • 김훈 (강원대학교 동물생명과학대학) ;
  • 곽경록 (강원대학교 동물생명과학대학) ;
  • 이효정 (강원대학교 동물생명과학대학) ;
  • 정동기 (제주대학교 생명공학부) ;
  • 손시환 (경남과학기술대학교 동물생명과학과) ;
  • 홍영호 (중앙대학교 생명자원공학부) ;
  • 이성진 (강원대학교 동물생명과학대학)
  • Yang, Song-Yi (College of Animal Life Sciences, Kangwon National University) ;
  • Choi, So-Young (College of Animal Life Sciences, Kangwon National University) ;
  • Hong, Min-Wook (College of Animal Life Sciences, Kangwon National University) ;
  • Kim, Hun (College of Animal Life Sciences, Kangwon National University) ;
  • Kwak, Kyeongrok (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Hyojeong (College of Animal Life Sciences, Kangwon National University) ;
  • Jeong, Dong Kee (Department of Animal Biotechnology, Jeju National University) ;
  • Sohn, Sea Hwan (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Hong, Yeong Ho (Department of Animal Science and Technology, Chung-Ang University) ;
  • Lee, Sung-Jin (College of Animal Life Sciences, Kangwon National University)
  • 투고 : 2018.07.10
  • 심사 : 2018.11.07
  • 발행 : 2018.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

$HNF4{\alpha}$ 유전자는 인간의 지질 수송 및 대사에 관여하는 간 전사인자로써, 닭의 지방 축적에 관련된 잠재적 후보유전자로 선정하였다. 선행연구에서 보고된 $HNF4{\alpha}$ 유전자 내 A543G SNP은 외래 육계뿐만 아니라, 외래 육계와 유전적 차이를 가지는 한국 재래계의 생시체중과 생체중에 유의적 연관성을 보인 바 있으나, 해당 SNP의 정확한 위치는 보고된 바 없다. 따라서 본 연구에서는 sequencing을 통하여 A543G SNP의 정확한 위치를 파악하고, A543G SNP의 주변 부위의 한국 재래계의 산육형질에 유의적 연관성이 있는 SNP을 파악하고자 하였다. Genomic DNA는 128수의 한국재래계의 혈액을 이용하여 추출하였으며, PCR 뒤 sequencing에 사용되었다. Sequencing 결과, 증폭범위 내에서 총 14개의 SNP가 탐색되었으며, 이 중 $HNF4{\alpha}$ 유전자의 intron 4 상에서 기존에 보고되지 않은 1개의 새로운 SNP를 발견하였다. 확인된 14개의 SNP 중 rs731246957과 rs736159604가 재래계의 생시체중 및 생체중에 유의적 연관성(P<0.001)을 보였다. rs731246957은 닭의 20번 염색체의 5,566,970번째에 위치하며, 선행논문에서 체중과의 연관성이 보고된 A543G SNP인 것으로 확인되었으며, 기존의 연구결과와 유사한 결과를 얻을 수 있었다. 반면에 선행논문에서 닭의 성장형질과의 연관성이 보고된 적 없는 rs736159604 SNP는 GG 유전자형 그룹이 GA, AA 유전자형 그룹에 비해 꾸준히 높은 체중 관측치를 보였으며, 특히 40주령의 체중은 다른 유전자형 그룹에 비해 약 1.8배 높게 관측되었다. 뿐만 아니라 신규 발견한 SNP의 T allele을 가지는 계군이 G allele을 가지는 계군보다 성장면에서 고능력을 보이는 것을 확인하였다. 따라서 본 연구결과는 한국재래계의 산육형질과 연관된 후보유전자로써 $HNF4{\alpha}$ 유전자의 기초정보를 제공하며, 해당 유전자내 특정 단일염기의 변이가 재래계의 체중 관련 유전자 마커로 유용하게 활용 가능할 것을 시사한다.

The hepatocyte nuclear factor 4 alpha ($HNF4{\alpha}$) gene is related to lipid transport, including abdominal fat and growth, in chickens. Interestingly, the A543G SNP within the $HNF4{\alpha}$ gene has previously been reported to be associated with body weight in both broilers and Korean native chickens (KNCs). However, its exact position within the HNF4 is not yet reported. This study aimed to identify the position of the A543G SNP and to identify additional SNPs that can be used as genetic markers in KNCs. A total of 128 KNCs were used for the sequencing and analysis of these genetic associations. As a result, A543G SNP was located in intron 4 of the $HNF4{\alpha}$ gene; it is reported as rs731246957 in the NCBI database. Fourteen SNPs were detected in the sequenced portion of the $HNF4{\alpha}$ gene; three of these, rs731246957, rs736159604 and new SNP, intron 6 (249), were significantly related with growth in the chickens. In this study, the TT genotype of rs731246957, previously reported as A543G SNP, the GG genotype of rs736159604 and GT of new SNP have are highly associated with body weight from birth to 40 weeks of age in KNCs (P<0.01). These results suggest that rs736159604, rs731246957 and intron 6 (249) SNPs within the $HNF4{\alpha}$ gene could function as growth-related markers in the selective breeding of KNCs.

키워드

참고문헌

  1. Ahmed AA, Musa HH, Sifaldin AZ, Musa TH, Fedail JF 2015 Hepatocyte nuclear factor 4-${\alpha}$, glucocorticoid receptor and heat shock protein 70 mRNA expression during embryonic development in chickens. J Anim Health Prod 3(3):54-58. https://doi.org/10.14737/journal.jahp/2015/3.3.54.58
  2. Argyrokastritis A, Kamakari S, Kapsetaki M, Kritis A, Talianidis I, Moschonas NK 1997 Human hepatocyte nuclear factor-4 (hHNF-4) gene maps to 20q12-q13. 1 between PLCG1 and D20S17. Human Genetics 99(2):233-236. https://doi.org/10.1007/s004390050345
  3. Choe JH, Nam KC, Jung S, Kim BN, Yun HJ, Jo CR 2010 Differences in the quality characteristics between commercial Korean native chickens and broilers. Korean J Food Sci Anim Resour 30(1):13-19. https://doi.org/10.5851/kosfa.2010.30.1.13
  4. Choi CH 2000 Feeding and management of Korean native commercial chickens that increased the meat productivity. Korean Poult J 32(10):87-92.
  5. Choi SY, Yang SY, Hong MW, Sohn SW, Jeong DK, Hong YH, Lee SJ 2016 Effect of SNP within HNF4${\alpha}$ associated with growth performance in Korean native chickens. Ann Anim Resour Sci 27(2):81-86. https://doi.org/10.12718/AARS.2016.27.2.81
  6. Ellard S, Colclough K 2006 Mutations in the genes encoding the transcription factors hepatocyte nuclear factor 1 alpha (HNF1A) and 4 alpha (HNF4A) in maturity-onset diabetes of the young. Human Mutation 27(9):854-869. https://doi.org/10.1002/humu.20357
  7. Handschin C, Gnerre C, Fraser DJ, Martinez-Jimenez C, Jover R, Meyer UA 2005 Species-specific mechanisms for cholesterol $7{\alpha}$-hydroxylase (CYP7A1) regulation by drugs and bile acids. Arch Biochem Biophys 434(1):75-85. https://doi.org/10.1016/j.abb.2004.10.010
  8. Jayasena DD, Jung S, Kim HJ, Bae YS, Yong HI, Lee JH, Jo C 2013 Comparison of quality traits of meat from Korean native chickens and broilers used in two different traditional Korean cuisines. Asian-Australas J Anim Sci 26(7):1038. https://doi.org/10.5713/ajas.2012.12684
  9. Jayasena DD, Kim SH, Lee HJ, Jung S, Lee JH, Park HB, Jo C 2014 Comparison of the amounts of taste-related compounds in raw and cooked meats from broilers and Korean native chickens. Poult Sci 93(12):3163-3170. https://doi.org/10.3382/ps.2014-04241
  10. Jeon HJ, Choe JH, Jung YK, Kruk ZA, Lim DG, Jo CR 2010 Comparison of the chemical composition, textural characteristics, and sensory properties of North and South Korean native chickens and commercial broilers. Korean J Food Sci Anim Resour 30(2):171-178. https://doi.org/10.5851/kosfa.2010.30.2.171
  11. Kim SW, Yoo M 2009 Association of hepatocyte nuclear factor-$4{\alpha}$ polymorphisms with type 2 diabetes in Koreans. J Life Sci 19(3):362-365. https://doi.org/10.5352/JLS.2009.19.3.362
  12. Kim YS, Byun MJ, Kim JH, Suh SW, Kim H, Do YJ, Kim JD, Choi SB 2013 Comparison of reproductive ability by age in weeks ans body weights of Korean native chicken cock. Ann Anim Resour Sci 24(2):97-101. https://doi.org/10.12718/AARS.2013.24.2.97
  13. Kwon EJ, Lee AA, You Y, Park H, Cho SJ, Ha EH, Kim YJ 2016 DNA methylations of MC4R and HNF4${\alpha}$ are associated with increased triglyceride levels in cord blood of preterm infants. Medicine 95(35):e4590. https://doi.org/10.1097/MD.0000000000004590
  14. Lee SS, Cha EY, Jung HJ, Shon JH, Kim EY, Yeo CW, Shin JG 2008 Genetic polymorphism of hepatocyte nuclear factor $4{\alpha}$ influences human cytochrome P450 2D6 activity. Hepatol 48(2):635-645. https://doi.org/10.1002/hep.22396
  15. Nam KC, Jo C, Lee M 2010 Meat products and consumption culture in the East. Meat Sci 86(1):95-102. https://doi.org/10.1016/j.meatsci.2010.04.026
  16. Pearson ER, Boj SF, Steele AM, Barrett T, Stals K, Shield JP, Hattersley AT 2007 Macrosomia and hyperinsulinaemic hypoglycaemia in patients with heterozygous mutations in the HNF4A gene. PLoS Med 4(4):e118. https://doi.org/10.1371/journal.pmed.0040118
  17. Silva VH, Pandolfi JRC, Godoy TF, Peixoto JO, Tessmann AL, Ledur MC 2012 A SNP in the hepatocyte nuclear factor 4 gene associated with carcass traits in males from a paternal broiler line. Worlds Poult Sci J, suppl. 1. GB_2012sp96_1.
  18. Silva VH, Pandolfi JRC, Godoy TF, Peixoto JO, Tessmann AL, Cantao ME, Ledur MC 2013 Hepatocyte nuclear factor 4 gene associated with bone traits and birth weight in male chickens. Page 27 In: Proceeding of Brazilian-International Congress of Genetics, Brazil.
  19. Wang S, Lan F, Huang L, Dong L, Zhu Z, Li Z, Fu J 2005 Suppression of hLRH-1 mediated by a DNA vector-based RNA interference results in cell cycle arrest and induction of apoptosis in hepatocellular carcinoma cell BEL-7402. Biochem Biophys Res Commun 333(3):917-924. https://doi.org/10.1016/j.bbrc.2005.05.186
  20. Wang ZQ, Lu FE, Leng SH, Fang XS, Chen G, Wang ZS, Yan ZQ 2008 Facilitating effects of berberine on rat pancreatic islets through modulating hepatic nuclear factor 4 alpha expression and glucokinase activity. World J Gastroenterol 14(39):6004. https://doi.org/10.3748/wjg.14.6004
  21. Woodfint RM, Chen PR, Ahn J, Suh Y, Hwang S, Lee SS, Lee K 2017 Identification of the MUC2 promoter as a strong promoter for intestinal gene expression through generation of transgenic quail expressing GFP in gut epithelial cells. Int J Mol Sci 18(1):196. https://doi.org/10.3390/ijms18010196
  22. Yang Q, Tang S, Dong L, Chen Q, Liu X, Jiang J, Deng Y 2014 Transcriptional regulation of chicken cytochrome P450 2D49 basal expression by CCAAT/enhancer binding protein ${\alpha}$ and hepatocyte nuclear factor $4{\alpha}$. FEBS J 281(5):1379-1392. https://doi.org/10.1111/febs.12710