Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Blood Pressure Reduction of SHR Treated with EA-contaning Lentinus edodes Mycelial Solid Culture Extract

Eritadenine 함유 표고버섯균사체 고체배양 효소추출물(EA-LEMSCEE)의 spontaneous hypertension rat 혈압감소

  • Moon, Yeon-Kyu (Gyeongnam Biological Resource Research Center, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology) ;
  • Jung, Jae Eun (Antiaging Institute, HK Biotech., Co., Ltd.) ;
  • Cho, Bok-Im (Gyeongnam Biological Resource Research Center, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology) ;
  • Heo, Jeong-Doo (Gyeongnam Biological Resource Research Center, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology) ;
  • Choi, Jungil (Gyeongnam Biological Resource Research Center, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology) ;
  • Kim, Jeong Ok (Antiaging Institute, HK Biotech., Co., Ltd.) ;
  • Ha, Yeong Lae (Antiaging Institute, HK Biotech., Co., Ltd.)
  • 문연규 (안전성평가연구소 경남환경독성본부 경남생명자원연구센터) ;
  • 정재은 ((주)HK바이오텍 항노화연구소) ;
  • 조복임 (안전성평가연구소 경남환경독성본부 경남생명자원연구센터) ;
  • 허정두 (안전성평가연구소 경남환경독성본부 경남생명자원연구센터) ;
  • 최정일 (안전성평가연구소 경남환경독성본부 경남생명자원연구센터) ;
  • 김정옥 ((주)HK바이오텍 항노화연구소) ;
  • 하영래 ((주)HK바이오텍 항노화연구소)
  • Received : 2017.12.27
  • Accepted : 2017.02.19
  • Published : 2018.02.28
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Abstract

Eritadenine (EA), derived from Lentinus edodes (LE), reduced low-density lipoprotein (LDL), triglyceride (TG), and phospholipids in bloods, and fatty acid depositions in animals and humans. Previously, we reported that EA inhibited angiotensin-converting enzyme (ACE) activity in vitro. Now, we report that EA reduced blood pressures in spontaneous hypertension rats (SHR). EA-containing LE mycelial culture enzyme extract (EA-LEMSCEE) was prepared from LE mycelial solid cultures and the hot-water extract of LE fruit bodies. Both EA and EA-LEMSCEE inhibited ACE activity in immortalized human umbilical endothelial cells (EA.hy926). EA-LEMSCEE treatments (7.5 mg/kg, 22.5 mg/kg) significantly reduced systolic and diastolic blood pressure in SHR. At five weeks of treatment, EA-LEMSCEE treatment significantly reduced systolic and diastolic blood pressure, similar to the positive control (captopril, CP; 4 mg/kg) treatment. In addition, the LEMSCEE without EA decreased systolic and diastolic blood pressures compared to the control, but not significant. EA-LEMSCEE decreased renin and ACE activities, and angiotensin II (Ang II) contents in SHR compared to the control. After five weeks of treatment, the effect of EA-LEMCEE was similar to that of CP. These results indicate that EA and EA-LEMSCEE reduce blood pressure by inhibiting the renin and ACE activity of SHR. Furthermore, these results imply that EA or EA-LEMSCEE could be used as an antihypertension agent in humans.

표고버섯으로부터 유래한 eritadenine (EA)은 혈중 low density lipoprotein (LDL), triglyceride (TG), phospholipid를 감소시키는 물질이다. 최근 저희 연구팀은 in vitro 연구에서 EA가 angiotensin converting enzyme (ACE)의 활성을 저해함을 밝혔다. 본 연구에서는 EA와 EA 함유한 표고버섯균사체 고체배양 효소추출물(EA-LEMCEE)이 본태성 고혈압 쥐 SHR의 혈압을 낮추었음을 보고한다. EA 및 EA-LEMSCEE는 인체 탯줄 혈관내피세포 EA.hy926 세포의 ACE 활성을 억제하였다. EA-LEMSCEE는 SHR의 수축기 및 이완기 혈압을 Control 처리구에 비해 유의성 있게 낮추어 처리 5주에는 Positive control인 captopril (CP) 처리와 비슷한 혈압감소 효과를 나타내었다. EA-LEMCE는 LEMSCEE에 비해 수축기 및 이완기 혈압을 감소시켰지만 유의성은 없었다. EA- LEMSCEE는 renin과 ACE 활성을 Control 처리에 비해 유의성 있게 억제하였고, angiotensin II (Ang II) 함량을 감소시켜, 처리 5주 후에 이러한 CP의 효과와 유사하였다. 또한 EA-LEMCEE는 LEMCEE에 비해 유의성 있게 renin 및 ACE 활성을 억제하였고 및 Ang II 함량을 감소시켰다. 이와 같은 결과는 EA 및 EA-LEMCEE는 본태성 고혈압 SHR의 renin과 ACE 활성을 억제하여 혈압을 낮추었고, EA 및 EA-LEMSCEE는 인체 고혈압을 낮출 수 있음을 의미한다.

Keywords

References

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