International Journal of Oral Biology

  • 제43권4호
  • /
  • Pages.193-200
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  • 2018
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Effect of Natural Extracts on Oral Care Probiotics Weissella cibaria CMU and Periodontal Pathogens

  • Yeu, Ji-Eun (Research Institute, Oradentics Inc.) ;
  • Kim, Hyun-Jin (Institute of Biomaterial Implant, Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Kang, Mi-Sun (Research Institute, Oradentics Inc.)
  • 투고 : 2018.11.01
  • 심사 : 2018.12.10
  • 발행 : 2018.12.31
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초록

The purpose of this study is to determine if natural extracts could be used as an additive in oral health food made with Weissella cibaria CMU (oraCMU). Natural extracts of green tea, mulberry leaf, licorice, and propolis, which are reported to have antimicrobial activities, were selected and used in this study. The minimum inhibitory concentrations (MIC) of extracts on periodontal pathogens such as Fusobacterium nucleatum and Porphyromonas gingivalis and their synergy effects with oraCMU by the fractional inhibitory concentrations methods were measured. From the results obtained, all the extracts showed no effect on the growth of oraCMU. Green tea extract showed the best antibacterial activity with MIC of 1.8 mg/ml against both F. nucleatum and P. gingivalis. In addition, green tea extract had a synergistic effect with oraCMU against F. nucleatum. Therefore, these results suggested that green tea extract is available as an additive in oral health food made with oraCMU.

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참고문헌

  1. Petersen PE, Ogawa H. Strengthening the prevention of periodontal disease: The WHO approach. J Periodontol. 2005;76:2187-2193. https://doi.org/10.1902/jop.2005.76.12.2187
  2. Byrne SJ, Dashper SG, Darby IB, Adams GG, Hoffmann B, Reynolds EC. Progression of chronic periodontitis can be predicted by the levels of Porphyromonas gingivalis and Treponema denticola in subgingival plaque. Oral Microbiol. Immunol. 2009;24:469-477. https://doi.org/10.1111/j.1399-302X.2009.00544.x
  3. Pham T. The association between periodontal disease severity and metabolic syndrome in Vietnamese patients. Int J Dent Hygiene. 2018;16:484-491. https://doi.org/10.1111/idh.12350
  4. Turton M, Africa CWJ. Further evidence for periodontal disease as a risk indicator for adverse pregnancy outcomes. Int Dent J. 2017;67:148-156. https://doi.org/10.1111/idj.12274
  5. Ionel A, Lucaciu O, Tbran F, Berce C, Toader S, Hurubeanu L, Bondor C, Cmpian RS. Histopathological and clinical expression of periodontal disease related to the systemic inflammatory response. Histol Histopathol. 2017;32:379-384.
  6. Kim TI, Choi EJ, Jeong JP, Han SB, Gu Y. Antimicorbial effect of Zea Mays L. and Magnoliae cortex extract mixtures on periodontal pathogen and effect on human gingival fibrobla. J. Kor. Academy Of Periodontology. 2002;32: 249-255. https://doi.org/10.5051/jkape.2002.32.1.249
  7. Van Strydonck DA, Timmerman MF, van der Velden U, van der Weijden GA. Plaque inhibition of two commercially available chlorhexidine mouthrinses. J Clin Periodontol. 2005;32:305-309. https://doi.org/10.1111/j.1600-051X.2005.00681.x
  8. Browne D, Whelton H, Denis O'Mullane. Fluoride metabolism and fluorosis. J Dent. 2005;33:177-186. https://doi.org/10.1016/j.jdent.2004.10.003
  9. Hara K, Ohara M, Hayashi I, Hino T, Nishimura R, Iwasaki Y, Ogawa T, Ohyama Y, Sugiyama M, Amano H. The green tea polyphenol (-)-epigallocatechin gallate precipitates salivary proteins including alpha-amylase: biochemical implications for oral health. Eur J Oral Sci. 2012;120: 132-139. https://doi.org/10.1111/j.1600-0722.2012.00947.x
  10. Min DJ, Yi SW, Lss Sh, Kim SS, Kim CH, Lee JH, Bae JH, Kim HK. The Anti-inflammatory effect of green tea extract against Prevotella intermedia. J. Soc. Cosmet. Scientists Korea. 2011;37:67-73.
  11. Choi JL, Jung MA, Jung SH. Antimicrobial effect of mulberry leaves extracts against oral microorganism. J Dent Hyg Sci. 2006;6:251-254.
  12. Islam B, Khan SN, Haque I, Alam M, Mushfiq M, Khan AU. Novel anti-adherence activity of mulberry leaves: inhibition of Streptococcus mutans biofilm by 1-deoxynojirimycin isolated from Morus alba. J Antimicrob Chemother. 2008; 62:751-757. https://doi.org/10.1093/jac/dkn253
  13. Koo H, Gomes BP, Rosalen PL, Ambrosano GM, Park YK, Cury JA. In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Arch Oral Biol. 2000;45: 141-148 https://doi.org/10.1016/S0003-9969(99)00117-X
  14. Ahn SJ, Cho EJ, Kim HJ, Park SN, Lim YK, Kook JK. The antimicrobial effects of deglycyrrhizinated licorice root extract on Streptococcus mutans UA159 in both planktonic and biofilm cultures. Anaerobe. 2012;18:590-596. https://doi.org/10.1016/j.anaerobe.2012.10.005
  15. Kang MS, Yeu JE, Oh JS, Shin BA, Kim JH. Complete genome sequences of Weissella cibaria strains CMU, CMS1, CMS2, and CMS3 isolated from infant saliva in South Korea. Microbiol Resour Announc. 2017;5:1-2.
  16. Kang MS, Chung J, Kim SM, Yang KH, Oh JS. Effect of Weissella cibaria isolates on the formation of Streptococcus mutans biofilm. Caries Res 2006;40:418-425. https://doi.org/10.1159/000094288
  17. Kang MS, Lim HS, Kim SM, Lim YJ, Lee HC, Oh JS. Quantitative analysis of Weissella cibaria against periodontopathic bacteria by real-time PCR. J Bacteriol Virol. 2009;39:111.
  18. Kang MS, Kim BG, Chung J, Lee HC, Oh JS. Inhibitory effect of Weissella cibaria isolates on the production of volatile sulphur compounds. J Clin Periodontol 2006;33:226-232. https://doi.org/10.1111/j.1600-051X.2006.00893.x
  19. Jeong HM, Kim YS, Ahn SJ, Auh MS, Ahn JB, Kim KY. Effects of Zizyphus jujuba var. boeunesis extracts on the growth of intestinal microflora and its antioxidant activities. J Korean Soc Food Sci Nutr. 2011;40:500-508. https://doi.org/10.3746/jkfn.2011.40.4.500
  20. Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard. Ninth ed. CLSI document M7-A8. Wayne Pennsylvania USA; 2012.
  21. Bonapace CR, Bosso JA, Friedrich LV, White RL. Comparison of methods of interpretation of checkerboard synergy testing. Diagn Microbiol Infect Dis. 2002;44: 363-366. https://doi.org/10.1016/S0732-8893(02)00473-X
  22. Harper DS, Mueller LJ, Fine JB, Gordon J, Laster LL. Effect of 6 months use of a dentifrice and oral rinse containing sanguinaria extract and zinc chloride upon the microflora of the dental plaque and oral soft tissues. J Periodontol. 1990;61:359-363. https://doi.org/10.1902/jop.1990.61.6.359
  23. Chae GC, Auh QS, Chun YH, Hong JP. Antibacterial activity of artemisa Capillaris THUNB on oral bacteria. J Oral Med Pain. 2009;34:169-177.
  24. Sakagami H, Tomomura M. Dental application of natural products. Medicines. 2018;5:21.doi:https://doi.org/10.3390/medicines5010020.
  25. Kobayashi R, Kobayashi T, Sakai F, Hosoya T, Yamamoto M, Kurita-Ochiai T. Oral administration of Lactobacillus gasseri SBT2055 is effective in preventing Porphyromonas gingivalisaccelerated periodontal disease. Sci Rep. 2017;7:545. doi: https://doi.org/10.1038/s41598-017-00623-9.
  26. Jang HJ, Kang MS, Yi SH, Hong JY, Hong SP. Comparative study on the characteristics of Weissella cibaria CMU and probiotic strains for oral care. Molecules. 2016;21:E1752. https://doi.org/10.3390/molecules21121752
  27. Kang MS, Piao MS, Shin BA, Lee HC, Oh JS. Adhesion of Weissella cibaria to the epithelial cells and factors affecting its adhesion. J Bacteriol Virol. 2006;36:151-157. https://doi.org/10.4167/jbv.2006.36.3.151
  28. Fournier-Larente J, Morin MP, Grenier D. Green tea catechins potentiate the effect of antibiotics and modulate adherence and gene expression in Porphyromonas gingivalis. Arch Oral Biolz. 2016;65:35-43. https://doi.org/10.1016/j.archoralbio.2016.01.014
  29. Park SN, Lim YK, Cho E, Jo E, Park PS, Kook JK. Antimicrobial activity of mulberry leaf against mutans streptococci and periodontopathogens. Int J Oral Biol. 2014; 39:201-206. https://doi.org/10.11620/IJOB.2014.39.4.201
  30. Kim SA, Chung HJ. Antimicrobial effects of propolis against oral microorganisms. Korean J. Food Sci. Technol. 2013; 45:370-375. https://doi.org/10.9721/KJFST.2013.45.3.370
  31. Van Vuuren SF, Suliman S, Viljoen AM. The antimicrobial activity of four commercial essential oils in combination with conventional antimicrobials. Lett Appl Microbiol. 2009;48: 440-446. https://doi.org/10.1111/j.1472-765X.2008.02548.x
  32. Braga LC, Leite AA, Xavier KG, Takahashi JA, Bemquerer MP, Chartone-Souza E, Nascimento AM. Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus. Can J Microbiol. 2005;51:541-547. https://doi.org/10.1139/w05-022