Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Tissue concentrations of quercitrin in spotted sea bass (Lateolabrax maculatus) after extended feeding with fish mint (Houttuynia cordata) extract

어성초 (Houttuynia cordata) 추출물을 장기간 투여한 점농어 (Lateolabrax maculatus)에서 조직내 quercitrin 잔류 농도

  • Bak, Su-Jin (East Sea Fisheries Research Institute, NIFS) ;
  • Bae, Jun Sung (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Lee, Chae Won (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University)
  • 박수진 (국립수산과학원동해수산연구소) ;
  • 배준성 (군산대학교 수산생명의학과) ;
  • 이채원 (군산대학교 수산생명의학과) ;
  • 박관하 (군산대학교 수산생명의학과)
  • Received : 2018.11.26
  • Accepted : 2018.12.04
  • Published : 2018.12.31
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Abstract

The Houttuynia cordata has been utilized for various beneficial purposes in humans mainly because of its potent antioxidant principle quercitrin present in this plant. This study examines the possibility of producing a functional sea food commodity containing active principle quercitrin by feeding H. cordata for a extended period. Spotted sea bass (Lateolabrax maculatus) were fed a diet containing H. cordata at 0.1-1.0% levels for 1 month and tissue concentrations of quercitrin were analyzed in serum, hepatopancreas and muscle. It was observed that quercitrin was found in the ranking order of hepatopancreas>muscle>serum. After a bolus administration of quercitrin (20 mg/kg, oral) to spotted sea bass and Nile tilapia (Oreochromis niloticus), idential rank order was observed after 48 hr. In contrast, the order was liver>serum>muscle in rat and mice, indicating that higher quercitrin distribution occurs to the muscle in fishes compared with in mammals tested. High residue concentration of qeurcitrin in the edible tissue can be an advantageous property in terms of functional food production. High level H. cordata extract inclusion of 1.0% seems to have detrimental effects in spotted sea bass leading to growth retardation and hepatic damage. It was concluded that incorporation of H. cordata extract into diet can be a way of producing healthy foods. However the level of active extract needs fine tuning to avoid toxicity to fishes.

어성초에서 발견되는 다양한 활성성분 중 강력한 항산화작용인 있는 quercitrin의 존재로 인해 건강식품이나 천연물의약품으로의 활용 목적으로 각광 받아 왔다. 본 연구에서는 어성초 추출물을 점농어의 사료에 첨가하여 장기간 급이함으로써, 가식부에 잔류하는 quercitrin으로 인해 식품의 가치를 높일 수 있는지 하는 가능성에 대한 연구를 수행하였다. 점농어에 어성초 추출물을 0.1, 0.5 및 1.0%의 수준으로 1개월간 급이하면 quercitrin이 hepatopancreas>muscle>serum의 순으로 잔류한다. 한편 Quercitrin을 순수물질 상태로 점농어, 틸라피아, 랫드 및 마우스에 20 mg/kg로 단회 경구 투여하고 48시간 후 조직내의 quercitrin 농도를 측정하면 점농어와 틸라피아에서는 어성초 추출물을 투여한 것과 동일한 잔류 농도 패턴을 보이지만 시험한 두 종의 포유동물에서는 liver>serum>muscle의 순으로 잔류 농도가 관찰된다. 이 결과로부터, quercitrin의 어류 가식부 근육내에서 발견된 상대적으로 높은 축적성은 기능성식품의 생산 측면에서 유리한 패턴의 특성으로 생각된다. 한편 최고 농도인 어성초 1.0%의 1개월간 투여 후 점농어의 성장에서 유의한 수준의 저해가 관찰되었으며 동시에 이 농도에서 간장 손상지표인 혈청내 효소의 변화도 관찰되었다. 결론적으로 어성초는 기능성 수산식품의 개발에 활용할 수 있는 가능성을 제시하지만 이때 어류에서 독성이 나타나지 않는 수준의 농도로 제한하는 것이 현명한 것으로 보인다.

Keywords

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