Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Rubus coreanus byproducts on intestinal microbiota and the immune modulation

  • Yu, Da Yoon (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Kim, Sung Hak (Department of Animal Science, Chonnam National University) ;
  • Kim, Jeong A (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Kim, In Sung (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Moon, Yang Soo (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Lee, Sang Suk (Department of Animal Science and Technology, Sunchon National University) ;
  • Park, Hwa Chun (Dasan Genetics) ;
  • Jung, Jong Hyun (Dasan Genetics) ;
  • Chung, Yi Hyung (Jeonbuk Institute for Bioindustry) ;
  • Shin, Dae Keun (Berry & Biofood Research Institute) ;
  • Nam, Ki Chang (Department of Animal Science and Technology, Sunchon National University) ;
  • Choi, In Soon (Department of Life Science, Silla University) ;
  • Cho, Kwang Keun (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
  • Received : 2017.10.02
  • Accepted : 2018.01.04
  • Published : 2018.03.01
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Abstract

Objective: Although the efficacy of Rubus coreanus (RC) byproducts as a feed additive has been recognized, its effects on intestinal microorganisms and the immune system are still unknown. Methods: Six-week-old male rats were treated with 0.5% RC (T1), 1.0% RC (T2), and 1.5% RC (T3) for 4 weeks. Results: We found that treatment with RC byproducts significantly increased the daily gain of body weight and feed intake. Treg-cell differentiation was enhanced in the mesenteric lymph nodes and spleen from the rats fed with RC byproducts. Illumina sequencing showed that bacteria in the phylum Firmicutes decreased and while those in the phylum Bacteroidetes increased in RC-treated groups. Particularly, the pathogenic microorganisms in the family Peptococcaceae decreased, and the non-pathogenic families Lachnospiraceae and S24-7 increased. Quantitative polymerase chain reaction analysis showed that the RC byproducts increased the lactic acid bacteria Bifidobacterium spp., Oscillospira spp., Leuconostoc citreum, and Weissella cibaria in a concentration-dependent manner. Conclusion: RC byproducts may be effective in immunomodulation by affecting intestinal microorganisms.

Keywords

References

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