DOI QR코드

DOI QR Code

Ginsenoside Rg3 promotes inflammation resolution through M2 macrophage polarization

  • Kang, Saeromi (Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University) ;
  • Park, Soo-Jin (Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University) ;
  • Lee, Ae-Yeon (Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University) ;
  • Huang, Jin (Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University) ;
  • Chung, Hae-Young (Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University) ;
  • Im, Dong-Soon (Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University)
  • 투고 : 2016.05.20
  • 심사 : 2016.12.22
  • 발행 : 2018.01.15

초록

Background: Ginsenosides have been reported to have many health benefits, including anti-inflammatory effects, and the resolution of inflammation is now considered to be an active process driven by M2-type macrophages. In order to determine whether ginsenosides modulate macrophage phenotypes to reduce inflammation, 11 ginsenosides were studied with respect to macrophage polarization and the resolution of inflammation. Methods: Mouse peritoneal macrophages were polarized into M1 or M2 phenotypes. Reverse transcription-polymerase chain reaction, Western blotting, and measurement of nitric oxide (NO) and prostaglandin $E_2$ levels were performed in vitro and in a zymosan-induced peritonitis C57BL/6 mouse model. Results: Ginsenoside $Rg_3$ was identified as a proresolving ginseng compound based on the induction of M2 macrophage polarization. Ginsenoside $Rg_3$ not only induced the expression of arginase-1 (a representative M2 marker gene), but also suppressed M1 marker genes, such as inducible NO synthase, and NO levels. The proresolving activity of ginsenoside $Rg_3$ was also observed in vivo in a zymosan-induced peritonitis model. Ginsenoside $Rg_3$ accelerated the resolution process when administered at peak inflammatory response into the peritoneal cavity. Conclusion: These results suggest that ginsenoside $Rg_3$ induces the M2 polarization of macrophages and accelerates the resolution of inflammation. This finding opens a new avenue in ginseng pharmacology.

키워드

참고문헌

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