한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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국내 신선 편이식품으로부터 분리한 Enterococcus의 항생제 저항성

Occurrence and antibiotic resistance of Enterococcus spp. from retail fresh-cut products in Korea

  • 김현정 (한국식품연구원 소비안전연구단) ;
  • 김승민 (한국방송통신대학교 생활과학과)
  • Kim, Hyun Jung (Research Group of Consumer Safety, Korea Food Research Institute) ;
  • Kim, Seung Min (Department of Human Ecology, Korea National Open University)
  • 투고 : 2018.10.16
  • 심사 : 2018.11.26
  • 발행 : 2018.12.31
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초록

본 연구에서는 서울과 경기도 지역 소매점 및 대형마트에서 판매되고 있는 샐러드, 어린잎, 새싹채소로부터 Enterococcus의 존재를 파악하고 9개 항생제에 대한 항생제 저항성 프로파일을 확보했다. Quinupristin/dalfopristin에 저항성을 가지는 분리주가 85.0%에 이르렀고, 특히 E. faecalis 분리주는 18종 중 17종이 저항성을 나타냈다. 또한 critically important 항생제 중 highest priority로 분류되는 ciprofloxacin과 erythromycin에 대해 각각 40.0, 50.0%가 중간 정도의 저항성을 보였으며, vancomycin에 대한 저항성을 가지는 분리주는 검출되지 않았다. Enterococci의 항생제 저항성 정도는 식품 안전뿐만 아니라 공중 보건에 있어서도 중요하다는 측면에서 본 연구의 Enterococcus 오염 정도, 항생제 내성율에 대한 결과는 향후 신선 편이식품 섭취가 인체 건강에 미치는 위험분석을 위한 기초자료로서 유용한 정보를 제공할 것으로 보이며 앞으로 특별한 열처리 없이 섭취하는 신선 편이식품에 대한 지속적인 모니터링이 필요할 것이라 사료된다.

Enterococcus spp. have been considered major indicator organisms for antibiotic resistance due to their ability to easily acquire and to harbor antibiotic resistance. In this study, Enterococcus spp. were isolated from 174 retail fresh-cut products (fresh vegetable salads, microgreens, and sprouts) in Korea. Among the 20 Enterococcus isolates obtained, 18 (90.0%) were Enterococcus faecalis and 2 (10.0%) were Enterococcus faecium. The patterns of antibiotic resistance against nine antimicrobials were analyzed. Most of the isolates (85.0%) were resistant to quinupristin/dalfopristin, 40.0% and 50.0% of the isolates showed intermediate resistance to two critically important antimicrobials for human medicine, ciprofloxacin and erythromycin, respectively. Vancomycin-resistant enterococci were not detected in this study. Given the importance of antimicrobial resistance of enterococci in food safety as well as in public health, our results regarding the occurrence (level of contamination) and antimicrobial resistance of Enterococcus spp. could provide useful information that aids the risk analysis of antibiotic resistance.

키워드

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Fig. 1. Comparison of antimicrobial resistance patterns of E. faecalis and E. faecium isolated from fresh-cut products (Veg) and pork meat products (Pork).

Table 1. Oligonucleotides used in this study

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Table 2. Occurrence of Enterococcus spp. in fresh-cut products

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Table 3. Classifications of antimicrobials used for susceptibility testing

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Table 4. Antibiotic resistance profiles of Enterococcus faecalis and Enterococcus faecium against 9 antibiotics

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Table 5. Drug resistance patterns of Enterococcus spp. isolated form fresh-cut products

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