Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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The Inhibitory Effect of L. plantarum Q180 on Adipocyte Differentiation in 3T3-L1 and Reduction of Adipocyte Size in Mice Fed High-fat Diet

  • Received : 2017.09.04
  • Accepted : 2017.12.28
  • Published : 2018.02.28
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Abstract

In this study, we examined the inhibitory effect of L. plantarum Q180 on adipocyte differentiation in 3T3-L1 and reduction of adipocyte size in mice fed high-fat diet. L. plantarum Q180 inhibited the adipocyte differentiation of 3T3-L1 cells ($18.47{\pm}0.32%$) at a concentration of $400{\mu}g/mL$ ($10^8CFU/g$). As a result of western blot analysis, the expression of $C/EBP{\alpha}$ and $PPAR{\gamma}$ in 3T3-L1 adipocyte treated with $400{\mu}g/mL$ of L. plantarum Q180 decreased 35.16% and 40.07%, respectively, compared with the control. To examine the effects, mice were fed three different diets as follows: ND (n=6) was fed ND and orally administered saline solution; HFD (n=6), HFD and orally administered saline solution; and HFD+Q180 (n=6), HFD and orally administered L. plantarum Q180. After six weeks, the rate of increase of body weight was 13.7% lower in the HFD+Q180 group compared to the HFD group. In addition, the epididymal fat weights of the HFD+Q180 group were lower than that of the HFD group. The change of adipocyte size was measured in diet-induced obese mice. Consequently, the number of large-size adipose tissue was less distributed in the ND and HFD+Q180 groups than in the HFD group. L. plantarum Q180 has an effect on the inhibition of 3T3-L1 adipocyte differentiation, fat absorption and reduction of adipocyte size. L. plantarum Q180 could be applied to functional food products that help improve obesity.

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References

  1. An HM, Park SY, Lee DK, Kim JR, Cha MK, Lee SW, Lim HT, Kim KJ, Ha NJ. 2011. Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats. Lipids Health Dis 10:116-124. https://doi.org/10.1186/1476-511X-10-116
  2. Bhathena J, Martoni C, Kunlamarva A, Urbanska AM, Malhotra M, Prakash S. 2009. Orally delivered micro encapsulated live probiotic formulation lowers serum lipids in hypercholesterolemic hamsters. J Med Food 12:310-319. https://doi.org/10.1089/jmf.2008.0166
  3. Cao Z, Umek RM, Mcknight SL. 1991. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Genes Dev 5:1538-1552. https://doi.org/10.1101/gad.5.9.1538
  4. Farmer SR. 2006. Transcriptional control of adipocyte formation. Cell Metab 4:263-273. https://doi.org/10.1016/j.cmet.2006.07.001
  5. Funatsu T, Kakuta H, Takasu T, Noguchi M, Suzuki M, Miyata K. 2003. Experimental model of postprandial hypertriglyceridemia in sucrose-fed rats and the effectiveness of atorvastatin in the model. Metabolism 52:609-615. https://doi.org/10.1053/meta.2003.50097
  6. Goor H, Gerrits PO, Grond J. 1986. The application of lipid-soluble stains in plastic embedded sections. Histochemistry 85:251-253. https://doi.org/10.1007/BF00494811
  7. Gregoire FM, Smas CM, Sul HS. 1998. Understanding adipocyte differentiation. Physiol Rev 78:783-809. https://doi.org/10.1152/physrev.1998.78.3.783
  8. Guo X, Liao K. 2000. Analysis of gene expression profile during 3T3-L1 preadipocyte differentiation. Gene 251:45-53. https://doi.org/10.1016/S0378-1119(00)00192-X
  9. Hamad EM, Sato M, Uze K, Yoshida T, Higashi S, Kawakami H, Kadooka Y, Matsuyama H, Abd El-Gawad IA, Imaizumi K. 2009. Milk fermented by Lactobacillus gasseri SBT 2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats. Br J Nutr 101:716-724. https://doi.org/10.1017/S0007114508043808
  10. Haslam DW, James WP. 2005. Obesity. Lancet 366:1197-1209. https://doi.org/10.1016/S0140-6736(05)67483-1
  11. Hemati N, Ross SE, Erickson RL, Groblewski GE, MacDougald OA. 1997. Signaling pathways through which insulin regulates CCAAT/enhancer binding protein-${\alpha}$ (C/EBP-${\alpha}$) phosphorylation and gene expression in 3T3-L1 adipocytes: correlation with CLUT4 gene expression. J Biol Chem 272:25913-25919. https://doi.org/10.1074/jbc.272.41.25913
  12. Hirose M, Ando T, Shofiqur R, Umeda K, Kodama Y, Nguyen SV, Goto T, Shimada M, Nagaoka S. 2013. Anti-obesity activity of hen egg anti-lipase immunoglobulin yolk, a novel pancreatic lipase inhibitor. Nutr Metab 10:70-75. https://doi.org/10.1186/1743-7075-10-70
  13. Hirsch J, Gallian E. 1968. Methods for the determination of adipose cell size in man and animals. J Lipid Res 9:110-119.
  14. Kim SJ, Cho SY, Kim SH, Song OJ, Shin IS, Cha DS, Park HJ. 2008. Effect of microencapsulation on viability and other characteristics in Lactobacillus acidophilus ATCC 43121. LWT-Food Sci Technol 41:493-500. https://doi.org/10.1016/j.lwt.2007.03.025
  15. Lee K, Paek K, Lee HY, Park JH, Lee Y. 2007. Antiobesity effect of trans-10, cis-12 conjugated linoleic acid-producing Lactobacillus plantarum PL62 on diet-induced obese mice. J Appl Microbiol 103:1140-1146. https://doi.org/10.1111/j.1365-2672.2007.03336.x
  16. Li Z, Jin H, Oh SY, Ji GE. 2016. Anti-obese effects of two Lactobacilli and two Bifidobacteria on ICR mice fed on a high fat diet. Biochem Biophys Res Commun 480:222-227. https://doi.org/10.1016/j.bbrc.2016.10.031
  17. Moon YS, Soh JR, Yu JJ, Sohn HS, Cha YS, Oh SH. 2012. Intracellular lipid accumulation inhibitory effect of Weissella koreensis OK1-6 isolated from kimchi on differentiating adipocyte. J Appl Microbiol 113:652-658. https://doi.org/10.1111/j.1365-2672.2012.05348.x
  18. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 62:55-63.
  19. Otto TC, Lane MD. 2005. Adipose development: from stem cell to adipocyte. Crit Rev Biochem Mol Biol 40:229-242. https://doi.org/10.1080/10409230591008189
  20. Park DY, Ahn YT, Huh CS, Jeon SM, Choi MS. 2011. The inhibitory effect of Lactobacillus plantarum KY1032 cell extract on the adipogenesis of 3T3-L1 Cells. J Med Food 14:670-675. https://doi.org/10.1089/jmf.2010.1355
  21. Park SY, Cho SA, Kim SH, Lim SD. 2014. Physiological characteristics and anti-obesity effect of Lactobacillus plantarum Q180 isolated from feces. Korean J Food Sci An 34:645-653.
  22. Ramirez-Zacarias JL, Castro-Munozledo F, Kuri-Harcuch W. 1992. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochem 97:493-497. https://doi.org/10.1007/BF00316069
  23. Rhyu J, Kim MS, You MK, Bang MA, Kim HA. 2014. Pear pomace water extract inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes. Nutr Res Pract 8:33-39. https://doi.org/10.4162/nrp.2014.8.1.33
  24. Rosen ED, Sarraf P, Troy AE, Brawin G, Moore K, Milstone DS, Spiegelman BM, Mortensen RM. 1999. PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Mol Cell 4:611-617. https://doi.org/10.1016/S1097-2765(00)80211-7
  25. Samuelsson L, Stromberg K, Vikman K, Bjursell G, Enerback S. 1991. The CCAAT/ enhancer binding protein and its role in adipocyte differentiation: Evidence for direct involvement in terminal adipocyte development. EMBO J 10:3787-3793.
  26. Takemura N, Okubo T, Sonoyama K. 2010. Lactobacillus plantarum strain No. 14 reduces adipocyte size in mice fed high-fat diet. Exp Biol Med 235:849-856. https://doi.org/10.1258/ebm.2010.009377
  27. Taranto MP, Medici M, Perdigon G, Holdago APR, Valdez GF. 1998. Evidence for cholesterolemic effect of Lactobacillus reuteri in hypercholesterolemic mice. J Dairy Sci 81:2336-2340. https://doi.org/10.3168/jds.S0022-0302(98)70123-7
  28. Tontonoz P, Hu E, Spiegelman BM. 1994. Stimulation of adipogenesis in fibroblasts by PPAR$\gamma$2, a lipid activated transcription factor. Cell 79:1147-1156. https://doi.org/10.1016/0092-8674(94)90006-X
  29. Wang YW, Jones PJ. 2004. Conjugated linoleic acid and obesity control: efficacy and mechanisms. Int J Obes Relat Metab Disord 28:941-955. https://doi.org/10.1038/sj.ijo.0802641

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