The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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The Effects of Gardenia jasminoides J.Ellis on Allergic Inflammation in RBL-2H3 Mast Cells and OVA/alum-sensitized Mice

쥐 비만세포 (RBL-2H3)와 OVA/alum 감작 마우스에서 치자 추출물의 알레르기 염증 억제 효과

  • Kim, Kyeong Ri (Department of Pediatrics, College of Korean medicine, Dajeon University) ;
  • Lee, Jin Hwa (Department of Pediatrics, College of Korean medicine, Dajeon University) ;
  • Kim, Eun Jin (Department of Pediatrics, College of Korean medicine, Dajeon University) ;
  • Kim, Yun Hee (Department of Pediatrics, College of Korean medicine, Dajeon University)
  • 김경리 (대전대학교 한의과대학 소아과학교실) ;
  • 이진화 (대전대학교 한의과대학 소아과학교실) ;
  • 김은진 (대전대학교 한의과대학 소아과학교실) ;
  • 김윤희 (대전대학교 한의과대학 소아과학교실)
  • Received : 2018.10.26
  • Accepted : 2018.11.19
  • Published : 2018.11.30
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Abstract

Objectives The purpose of this study is to investigate the effects of Gardenia jasminoides for. grandiflora extracts' (GAJ) anti-inflammatory effect on RBL-2H3 mast cells and OVA/alum-sensitized mice. Methods In this study, IL-4 and IL-13 production was measured via ELISA analysis, and mRNA expressions of GM-CSF, IL-4, IL-5, $TNF-{\alpha}$, IL-6 were analyzed by real-time PCR. In addition, MAPKs and $NF-{\kappa}B$ p65 transcription factors were examined using western blotting, and ELISA was used to understand IgE, IL-4, and IL-13 production in ovalbumin-allergic mice in in vitro study. Results As a result of this study, 1. GAJ were observed to suppress the mRNA expression of GM-CSF, IL-4, IL-13, IL-5, $TNF-{\alpha}$, IL-6 in comparison to PMA 50 ng/ml, ionomycin $0.5{\mu}M$ (PI) control group. 2. GAJ also inhibited the IL-4, IL-13 production in comparison to PI control group. 3. Western blot analysis showed decrease on the expression of mast-cell-specific transcription factors, including MAKPs (ERK, JNK, p38) and $NF-{\kappa}B$ p65. 4. Orally-administered GAJ group in OVA/alum induced Balb/c mice showed decreased level of OVA-specific IgE in the serum. This group also has shown decreased the level of IL-4, IL-13 in the splenocyte culture supernatant. Conclusions Obtained results suggest that GAJ may regulate the allergic inflammation by transcription factors MAKPs (ERK, JNK, p38) and $NF-{\kappa}B$ p65 causing inhibition of Th2 cytokines in mast cells and OVA/alum-sensitized mice.

Keywords

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Fig. 1. Cytotoxic effects of GAJ on RBL-2H3 mast cells

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Fig. 2. Suppressive effects of GAJ on GM-CSF mRNA expression

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Fig. 3. Suppressive effects of GAJ on IL-4 mRNA expression

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Fig. 4. Suppressive effects of GAJ on IL-13 mRNA expression

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Fig. 5. Suppressive effects of GAJ on IL-5 mRNA expression

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Fig. 6. Suppressive effects of GAJ on TNF-a mRNA expression

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Fig. 7. Suppressive effects of GAJ on IL-6 mRNA expression

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Fig. 8. Suppressive effects of GAJ on IL-4 production

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Fig. 9. Suppressive effects of GAJ on IL-13 production

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Fig. 10. Effects of GAJ on ERK phosphorylation in PI-induced RBL-2H3 mast cells

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Fig. 11. Effects of GAJ on p38 phosphorylation in PI-induced RBL-2H3 mast cells

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Fig. 12. Effects of GAJ on JNK phosphorylation in PI-induced RBL-2H3 mast cells

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Fig. 13. Effects of GAJ on the nuclear translocation of NF-κB p65 in PI-induced RBL-2H3 mast cells

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Fig. 14. Effect of GAJ on OVA-specific IgE release in OVA challenged allergic mouse model

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Fig. 15. Effect of GAJ on IL-4 release in release in OVA challenged isolated splenocytes

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Fig. 16. Effect of GAJ on IL-13 release in release in OVA challenged isolated splenocytes

Table 1. Rat Primer Sequence for Real-Time PCR Analysis

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