Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Antimicrobial Effect on Streptococcus mutans in Photodynamic Therapy using Different Light Source

광원의 종류에 따른 광역동 치료시의 Streptococcus mutans에 대한 항균 효과

  • Kim, Jaeyong (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Howon (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Juhyun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Seo, Hyunwoo (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Siyoung (Department of Oral Microbiology, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
  • 김재용 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 박호원 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 이주현 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 서현우 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 이시영 (강릉원주대학교 치과대학 미생물학교실 및 구강과학연구소)
  • Received : 2017.08.14
  • Accepted : 2017.09.12
  • Published : 2018.02.28
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Abstract

In a photodynamic therapy, the difference of antibacterial capacity was compared according to the type of source of light when the same quantity of energy is irradiated. After S. mutans is formed in planktonic state and biofilm state, erythrosine diluted to $40{\mu}M$ was treated for 3 minutes, and as the type of light source, Halogen, LED, and Plasma arc were used, which were irradiated for 30 seconds, 15 seconds and 9.5 seconds, respectively. After the completion of the experiment, CFU of each experiment arm was measured to compare the photodynamic therapeutic effects according to each condition. The CFU of each experiment arm had no statistically significant difference. Under the same quantity of energy, the photodynamic therapeutic effect can be said to be the same regardless of types of light source, which is a useful result in the clinical field with various light irradiators.

본 연구의 목적은 치면세균막 착색제인 erythrosine을 광감각제로 사용하여 S. mutans에 광역동 치료를 시행하였을 때, 동일한 에너지량을 조사시 광원의 종류에 따른 항균능의 차이를 비교해보고자 함이다. S. mutans를 각각 planktonic 상태와 biofilm 상태로 형성한 후 $40{\mu}M$로 희석한 erythrosine을 3분간 처리한 다음 광원의 종류로 Halogen, LED, Plasma arc를 사용하였고 각각 30초, 15초, 9.5초의 광조사를 시행하였다. 실험 종료 후 각 실험군의 CFU를 측정하여 각 조건에 따른 광역동 치료 효과를 비교하였다. 각 실험군의 CFU는 통계적으로 유의한 차이가 없었다. 동일 에너지량을 조사시 광역동 치료의 효과는 광원의 종류와 관계없이 동일하다고 할 수 있으며, 이는 다양한 광 조사 기기를 가진 임상현장에서도 유용한 결과라 할 수 있다.

Keywords

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