한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제26권1호
  • /
  • Pages.42-54
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  • 2018
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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한국 약용식물자원 추출물의 항알레르기 활성 평가 및 선발

Screening and Evaluation of the Anti-allergic Effect of Korean Medicinal Plant Extracts

  • 김난솔 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최두진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최은주 (대구가톨릭대학교 사범대학) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박샛별 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이재원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이대영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김금숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이승은 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • 투고 : 2017.10.10
  • 심사 : 2018.02.12
  • 발행 : 2018.02.28
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초록

Background: Allergic diseases like such as atopic dermatitis, asthma, and rhinitis have recently increased both domestically and globally. The present study was undertaken to select candidates with anti-allergic activity from plant resources. Methods and Results: Fifty-six plant extracts at $20{\mu}g/m{\ell}$ were screened against ${\beta}$-hexosaminidase production and interleukin (IL)-4 release in degranulated rat basophilic leukemia (RBL)-2H3 cells. The anti-allergy activity of three plant extracts selected from the preliminary screening experiment, Polygonatum sibiricum F. Delaroche (root), Pyrus pytifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud (root) were measured at concentrations of $2-250{\mu}g/m{\ell}$ in three cell lines as RBL-2H3, HaCaT and Jurkcat T cells. The assay showed the root extract of R. glutinosa to have an inhibitory activity of 4.2% - 28.6% on ${\beta}$-hexosaminidase production from IgE-sensitized RBL-2H3 cells. Each extract of P. sibiricum and R. glutinosa reduced IL-4 release in IgE-sensitized RBL-2H3 cells, respectively. The leaf extract of P. pyrifolia var. culta showed a significantly potent suppressive effect of 10.2% - 74.7% on the mRNA expression of tumor necrosis factor (TNF)-${\alpha}$ in HaCaT cells sensitized with TNF-a and INF-g, and showed inhibitory effect of -8.6% - 90.9% on the mRNA expression of IL-2 in Jurkat T cells sensitized with PMA and A23187. Conclusions: The results showed that the root of R. glutinosa and leaf of P. pyrifolia var. culta could be useful candidates as antiallergy materials.

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참고문헌

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