Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Screening and Evaluation of the Anti-allergic Effect of Korean Medicinal Plant Extracts

한국 약용식물자원 추출물의 항알레르기 활성 평가 및 선발

  • 김난솔 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최두진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최은주 (대구가톨릭대학교 사범대학) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박샛별 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이재원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이대영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김금숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이승은 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2017.10.10
  • Accepted : 2018.02.12
  • Published : 2018.02.28
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Abstract

Background: Allergic diseases like such as atopic dermatitis, asthma, and rhinitis have recently increased both domestically and globally. The present study was undertaken to select candidates with anti-allergic activity from plant resources. Methods and Results: Fifty-six plant extracts at $20{\mu}g/m{\ell}$ were screened against ${\beta}$-hexosaminidase production and interleukin (IL)-4 release in degranulated rat basophilic leukemia (RBL)-2H3 cells. The anti-allergy activity of three plant extracts selected from the preliminary screening experiment, Polygonatum sibiricum F. Delaroche (root), Pyrus pytifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud (root) were measured at concentrations of $2-250{\mu}g/m{\ell}$ in three cell lines as RBL-2H3, HaCaT and Jurkcat T cells. The assay showed the root extract of R. glutinosa to have an inhibitory activity of 4.2% - 28.6% on ${\beta}$-hexosaminidase production from IgE-sensitized RBL-2H3 cells. Each extract of P. sibiricum and R. glutinosa reduced IL-4 release in IgE-sensitized RBL-2H3 cells, respectively. The leaf extract of P. pyrifolia var. culta showed a significantly potent suppressive effect of 10.2% - 74.7% on the mRNA expression of tumor necrosis factor (TNF)-${\alpha}$ in HaCaT cells sensitized with TNF-a and INF-g, and showed inhibitory effect of -8.6% - 90.9% on the mRNA expression of IL-2 in Jurkat T cells sensitized with PMA and A23187. Conclusions: The results showed that the root of R. glutinosa and leaf of P. pyrifolia var. culta could be useful candidates as antiallergy materials.

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References

  1. Ban HJ, Park DI and Kang KH. (2012). Inhibitory effect of Artemisiae asiaticae herba on degranulation, production of cytokine, and $Fc{\varepsilon}RI$ expression in RBL-2H3 cells. Korean Journal of Oriental Physiology and Pathology. 26:915-921.
  2. Brown JM, Wilson TM and Metcalfe DD. (2007). The mast cell and allergic disease: Role in pathogenesis and implications for therapy. Clinical and Experimental Allergy. 38:4-18.
  3. Cheng C, Ng DSW, Chan TK, Guan SP, Ho WE, Koh AHM, Bian JS, Lau HYA and Wong WSF. (2013). Anti-allergic action of anti-malarial drug artesunate in experimental mast cell-mediated anaphylactic models. Allergy. 68:195-203.
  4. Choi JK, Jang YH, Lee S, Lee SR, Choi YA, Jin M, Choi JH, Park JH, Park PH, Choi H, Kwon TK, Khang D and Kim SH. (2017). Chrysin attenuates atopic dermatitis by suppressing inflammation of keratinocytes. Food and Chemical Toxicology. 110:142-150.
  5. Jeong YJ, Yang YJ and Kang SC. (2013). Constituent alterations of Pueraiae radix and lacquer tree mixture extract and their anti-allergic effects. Korean Journal of Plant Research. 26:103-110.
  6. Jung EM, Kim JW, Park MJ, Lee SS, Choi DH, Lee JB and Jeung EB. (2013). Inhibitory effect of extracts from Rbododendron Brachycarpum and Abies Koreana E. H. Wilson on degranulation and cytokine expression in RBL-2H3 cells. Journal of Korean Wood Science and Technology. 41:551-558.
  7. Jung JK and Park YK. (2011). Effect of modified- Okbyungpoongsan on mast cell-mediated allergic responses in RBL-2H3 mast cells. Korean Journal of Herbology. 26:1-7.
  8. Kang KH and Lee SY. (2012). Inhibitory effect of Fagopyrum esculentum on degranulation and production of cytokine in RBL-2H3 cells. Journal of Korean Oriental Medical Ophthalmology and Otolaryngology and Dermatology. 25:1-12.
  9. Kawakami T, Ando T, Kimura M, Wilson BS and Kawakami Y. (2009). Mast cells in atopic dermatitis. Current Opinion in Immunology. 21:666-678.
  10. Kim YJ, Park SJ and Kim TJ. (2011). Anti-allergic effects of nodakenin in IgE/Ag-induced type I hypersensitivity. Journal of Life Science. 21:1721-1725.
  11. Kim YM. (2010). The inhibitory effect of Agrimonia pilosa Ledeb extract on allergic reaction. Korean Journal of Medicinal Crop Science. 18:398-404.
  12. Lee JH, Mun SH, Ko NY, Kim JW, Kim DK, Kim JD, Her E and Choi WS. (2007). Roles of Src-family kinase isoforms, Lyn, Fyn, Fgr, and c-Src on degranulation in RBL-2H3 mast cells. Journal of Life Science. 17:350-355.
  13. Lee JS, Kim IS, Kim JH, Kim JS, Kim SH and Yun CY. (2008). Suppressive effects of Houttuynia cordata Thunb (Saururaceae) extract on Th2 immune response. Journal of Ethnopharmacology. 117:34-40.
  14. Lee YS, Han JK and Kim YH. (2012). Anti-allergic effects of Gagam-YangGyeokSan on RBL-2H3 mast cells and OVA/alum sensitized mice. Journal of Korean Orient Pediatrics. 26:10-23.
  15. Lim HB and Kim SH. (2012). Antiasthmatic effects on Scutellaria baicalensis Georgi extracts against airway inflammation and hyperreponsiveness induced by diesel exhaust particles with ovalbumin sensitization. Korean Journal of Medicinal Crop Science. 20:129-135.
  16. Lyu JH, Yoon HJ, Hong SH and Ko WS. (2008). Xanthium strumarium suppresses degranulation and pro-inflammatory cytokines secretion on the mast cells. Journal of Korean Oriental Medical Ophthalmology and Otolaryngology and Dermatology. 21:82-93.
  17. Na BR, Kim HR, Kwon MS, Lee HS, Piragyte I, Choi EJ, Choi HK, Han WC, Lee SH and Jun CD. (2013). Aplotaxene blocks T cell activation by modulation of protein kinase C-$\theta$-dependent pathway. Food and Chemical Toxicology. 62:23-31.
  18. Nishida K, Yamasaki S, Ito Y, Kabu K, Hattori K, Tezuka T, Nishizumi H, Kitamura D, Goitsuka R, Geha RS, Yamamoto T, Yagi T and Hirano T. (2005). $Fc{\varepsilon}RI$-mediated mast cell degranulation requires calcium-independent microtubule-dependent translation of granules to the plasma membrane. Journal of Cell Biology. 170:115-126.
  19. Park SB, Kang KH, Yoon HJ and Ko WS. (2011). Inhibitory effect of Ulmus davidiana on ${\beta}$-hexosaminidase release and cytokine production in RBL-2H3 cells. Journal of Korean Oriental Medical Ophthalmology and Otolaryngology and Dermatology. 24:86-95.
  20. Pyo SN and Sohn EH. (2008). Introduction to immunology. Shinilbooks, Seoul. Korea. p. 253-259.
  21. Wedemeyer J, Tsai M and Galli SJ. (2000). Roles of mast cells and basophils in innate and acquired immunity. Current Opinion Immunology. 12:624-631.
  22. Williams CMM and Galli SJ. (2000). The diverse potential effector and immunoregulatory roles of mast cells in allergic disease. Journal of Allergy and Clinical Immunology. 105:847-859.
  23. Wu H, Qi H, Iwasaki D, Zhu B, Shimoishi Y, Murata Y and Nakamura Y. (2009). JNK-dependent NFATc1 pathway positively regulates IL-13 gene expression induced by (-)-epigallocatechin-3-gallate in human basophilic KU812 cells. Free Radical Biology and Medicine. 47:1028-1038.
  24. Xu C, Wyman AR, Alaamery MA, Argueta SA, Ivey FD, Meyers JA, Lerner A, Burdo TH, Connolly T, Hoffman CS and Chiles TC. (2016). Anti-inflammatory effects of novel barbituric acid derivatives in T lymphocytes. International Immunopharmacology. 38:223-232.
  25. Zeng HR, Wang B, Zhao Z, Zhang Q, Liang MY, Yao YQ, Bian K and Zhang WR. (2016). Effects of Viola yedoensis Makino anti-itching compound on degranulation and cytokine generation in RBL-2H3 mast cells. Journal of Ethnopharmacology. 189:132-138.