Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of Fetal Bovine Serum Concentration on the Propagation of Korean Viral Haemorrhagic Septicaemia Virus in an Epithelioma Papulosum Cyprini Cell Line

우리나라에서 분리된 바이러스성출혈성패혈증 바이러스(Viral Haemorrhagic Septicaemia Virus, VHSV)의 증식에 대한 Fetal Bovine Serum (FBS) 농도의 영향

  • Kim, Jin Hui (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Park, Jeong Su (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Kwon, Se Ryun (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Kim, Shin Hu (National Fishery Products Quality Management Services) ;
  • Kim, Hyoung Jun (National Fishery Products Quality Management Services)
  • 김진희 (선문대학교 수산생명의학과) ;
  • 박정수 (선문대학교 수산생명의학과) ;
  • 권세련 (선문대학교 수산생명의학과) ;
  • 김신후 (국립수산물품질관리원) ;
  • 김형준 (국립수산물품질관리원)
  • Received : 2018.01.12
  • Accepted : 2018.02.06
  • Published : 2018.02.28
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Abstract

Fetal bovine serum (FBS) is essential for cell culture and is used in the determination of infectivity titer and propagation of viruses. To clarify the effects of FBS on the propagation of viral haemorrhagic septicaemia virus (VHSV), which is a causative agent of mass mortalities of olive flounder Paralichthys olivaceus in Korea, VHSV was inoculated into an EPC (epithelioma papulosum cyprinid) cell line supplemented with MEMs (minimal essential medium) with FBS concentrations of 0%, 2%, 5%, and 10% (MEM0, MEM2, MEM5, and MEM10), respectively, and infectivity titers were compared. Cytopathic effects were observed in all experimental groups at 2 days post virus inoculation (dpi) and all cells were detached from cell culture flasks at 7 dpi. Infectivity titers increased to 3 dpi, persisted to 7 dpi, and decreased when cells were detached. The titer of VHSV in EPC cells in MEM0 was the lowest while those in the other experimental groups showed similar levels. In conclusion, 2% (v/v) of FBS was sufficient to propagate VHSV in EPC cells and the withdrawal of VHSV from cell culture flasks should be performed before cell detachment.

Keywords

References

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