문화기술의 융합 (The Journal of the Convergence on Culture Technology)

  • 제4권4호
  • /
  • Pages.325-329
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  • 2018
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  • 2384-0358(pISSN)
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  • 2384-0366(eISSN)
국제문화기술진흥원 (The International Promotion Agency of Culture Technology)
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오옥신과 사이토키닌 호르몬 처리를 이용한 칼라 기내 식물체 대량증식체계 확립

Establishment of propagation system for in vitro calla plants(Zantedeschia spp.) by using auxin and cytokinin hormones treatments

  • 이상희 (건국대학교 생명공학과) ;
  • 김영진 (강원도농업기술원 원예연구과) ;
  • 양환래 (건국대학교 생명공학과) ;
  • 김종보 (건국대학교 생명공학과)
  • 투고 : 2018.08.02
  • 심사 : 2018.09.20
  • 발행 : 2018.11.30
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초록

천남성과에 속한 칼라는 생육 조건에 따라 두 종류로 구분이 가능한데 그 중 꽃 모양과 화색이 다양한 유색 칼라는 절화로서 인기가 매우 높다. 그러나 유색 칼라는 자연 번식률이 매우 낮고 개화구근을 얻기 까지 오랜 시간이 소요되어 우량 품종의 대량 번식 체계 확립이 시급하다. 따라서 본 연구에서는 칼라 기내 식물체의 생육을 향상시키고자 다양한 식물 성장 호르몬을 처리하여 실험을 진행하였다. 뿌리 발생은 기본 MS 배지에서 81.5 %로 가장 높았고 신초 및 다신초 발생률은 BA 2.0 mg/L와 IBA 1.0 mg/L 혼용 처리구에서 100.0 %와 36.4 %로 가장 높았다. 또한 생체중에서는 BA 2.0 mg/L 단일 처리구에서 11.2배가 증가하여 모든 처리구중 가장 높은 증가율을 보였다. Auxin과 cytokinin 혼용 처리 실험은 이전 연구에서도 많이 이용되고 있고 칼라 식물 생장에도 긍정적인 효과를 줌으로써 미소대량증식체계 확립에 도움을 줄 것이라고 판단된다.

There are two kinds of calla belonging to the Zantedeschia spp. depending on the growing condition. Between them, various colored calla with flower shape and flower color are very popular as cut flowers. However, colored calla is very low in natural reproduction rate, and it takes a long time to obtain a flowering bulb. So it is urgent to establish a large breeding system of good varieties. In this study, various plant growth hormone treatments were carried out to improve the growth of the plants in calla. Root formation was the highest at 81.5 % in basal MS medium, and the formation of shoot and multiple shoots were the highest at 100.0 % and 36.4 % in the combined treatment of BA 2.0 mg/L and IBA 1.0 mg/L, respectively. In fresh weight, the highest growth rate was observed with 11.2 times increase in BA 2.0 mg/L single treatment. Auxin and cytokinin mixed treatment were widely used in previous studies and positive effects on the growth of calla plants will help to establish the micro-propagation system.

키워드

GJMGCK_2018_v4n4_325_f0001.png 이미지

그림 1. 댜앙한 호르몬 첨가 배지에서 ‘각시’ 경정배양 8주 후 뿌리, 신초, 다신초 생장 효과 Figure 1. Effect of different medium on root, shoot, multiple shoots growth and proliferation in ‘Gagsi’ after 8 weeks of culture

GJMGCK_2018_v4n4_325_f0002.png 이미지

그림 2. 댜앙한 호르몬 첨가 배지에서 ‘각시’ 경정배양 후 생체중 증가 비교 Figure 2. Effect of different medium on increasing index of fresh weight in ‘Gagsi’

표 1. 댜앙한 호르몬 첨가 배지에서 ‘각시’ 뿌리, 신초, 다신초 생장률 Table 1. Effect of different medium on formation rates of root, shoot, multiple shoots in ‘Gagsi’

GJMGCK_2018_v4n4_325_t0001.png 이미지

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