The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Non-alcoholic fatty liver protective effects, and studies on the mechanism of action of Crataegi Fructus

산사의 NAFLD 보호 효과 및 그 작용기전에 관한 연구

  • Kim, Min-Chul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kong, Ryong (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Han, Hyoung-Sun (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kang, Dam-hee (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Lee, Seung-Jin (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Lee, Cheon-Cheon (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Wang, Seo (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kang, Ok-Hwa (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
  • 김민철 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 공룡 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 한형선 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 강담희 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 이승진 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 이천천 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 왕서 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 권동렬 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 강옥화 (원광대학교 약학대학 한약학과, 원광한약연구소)
  • Received : 2018.08.31
  • Accepted : 2018.11.25
  • Published : 2018.11.30
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Abstract

Objectives : Non-alcoholic fatty liver disease (NAFLD) is characterized by the accumulation of hepatic triglycerides (TG) that leads to inflammation and fibrosis. Crataegi Fructus ethanol extract (CE) is a korean traditional herb that used for digestive diseases. It has been investigated that CE has the effect that prevent hepatotoxicity caused by CCl4 or GaIN and regulate the inflammatory in several organs. However, a hypolipidemic effect of CF has not been reported. Methods : The purpose of this study is that examine the lipid accumulation inhibitory effect of CE on NAFLD. We checked the body and liver weight change of MCD-diet induced mice with/without administration of CE. The blood lipid levels of C57BL/6J mice were checked by biochemistry. Also we observed the liver histology of MCD-diet induced mice and investigate the molecular mechanisms in MCD-diet-induced NAFLD in C57BL/6J mice. Results : CE improved MCD-diet-induced lipid accumulation and TG and TC levels. Also, CE decreased hepatic lipogenesis such as SREBP-1, $C/EBP{\alpha}$, $PPAR{\gamma}$, ACC and FAS. Besides, we also found out that CE increased AMPK phosphorylation. These results indicated that CE has the same ability to activate AMPK and then reduce SREBP-1, and FAS expression, finally leading to inhibit hepatic lipogenesis and hepatic antioxidative ability. Conclusions : In this report, we found CE exerted a regulatory effect on lipid accumulation by decreasing lipogenesis in MCD-diet induced NAFLD model. Therefore, CE extract may be active in the prevention of fatty liver.

Keywords

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Figure 1. Body weight change of MCD-diet induced mice with/without treatment.

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Figure 2. Liver weight changes of each experimental groups.

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Figure 3. Effects of CE on blood lipid levels in C57BL/6J mice by biochemistry.

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Figure 4. The effect of CE on the liver histology in MCD-diet-induced mice.

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Figure 5. Effects of CE on MCD-diet induced liver function in C57BL/6J mice by biochemistry.

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Figure 6. Effects of CE on hepatic lipid accumulation related protein and mRNA expression in liver tissue of experimental mice.

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Figure 7. Effects of CE on lipogenesis related protein expression in liver tissue of experimental mice.

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Figure 8. Effects of CE on AMPK phosphorylation in liver tissue of experimental mice.

Table 1. Sequences of oligonucleotide primers designed for PCR.

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