Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Discriminability of Molecular Markers Based on Muclear Ribosomal ITS Sequences of Fagopyrum esculentum and F. tataricum

단메밀과 쓴메밀의 ITS 염기서열 기반 식별마커

  • 오대주 ((재)제주테크노파크 생물종다양성연구소) ;
  • 현호봉 ((재)제주테크노파크 생물종다양성연구소) ;
  • 임태준 ((재)제주테크노파크 생물종다양성연구소) ;
  • 윤선아 ((재)제주테크노파크 생물종다양성연구소) ;
  • 함영민 ((재)제주테크노파크 생물종다양성연구소) ;
  • 윤원종 ((재)제주테크노파크 생물종다양성연구소) ;
  • 양우삼 (제주특별자치도 농업기술원) ;
  • 정용환 ((재)제주테크노파크 생물종다양성연구소)
  • Received : 2018.10.12
  • Accepted : 2018.11.15
  • Published : 2018.11.30
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Abstract

We analyzed the nuclear ribosomal internal transcribed spacer (ITS) sequence of common buckwheat, Fagopyrum esculentum and tartary buckwheat, F. tataricum. The diversity of the nucleotides and haplotypes, Tajima's D, and Fu's Fs was analyzed and compared among the varieties of common buckwheat and tartary buckwheat. The diversity of nucleotides and haplotypes indicated that the buckwheat populations had undergone rapid population expansion but D and Fs did not support their expansion statistically. The phylogenetic analysis of ITS sequences did not clearly establish the phylogenetic relationships between the varieties of common buckwheat. The In/Del sequence of ITS-1 region could, therefore, be used as a DNA marker to distinguish raw or manufactured products derived from common buckwheat and tartary buckwheat.

Keywords

References

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