FIG. 1. Diagram of the HiLo optical imaging technique in the Fourier domain: (a) absolute difference value of uniform image and structured image, (b) obtaining the HiLo image by applying a low pass filter (LPF) to D and a high pass filter (HPF) to the uniform image.
FIG. 2. Experimental setup for the HiLo optical imaging technique. Structured illumination image and uniform illumination image are taken sequentially by moving the grating filter.
FIG. 3. Illustration of curve fitting to determine the focal plane.
FIG. 4. Lateral resolution comparison of (a) WFM, (b) HiLo by grid period, and (c) LSCM. FWHM for WFM, HiLo, and LSCM is 0.77 μm, 0.38 μm, and 0.25 μm, respectively. Target: USAF-1951 Positive, group 7 elements 6; objective lens: 50×, NA = 0.95; wavelength of light: WFM & HiLo 440 nm, LSCM 405 nm.
FIG. 5. Axial intensity profile comparison of HiLo optical imaging technique (black circles and line) and general WFI (yellow triangles and line) using a protected silver mirror. The Stokseth OTF model (dashed line) is also presented as the theoretical value.
FIG. 6. Variation of axial intensity profile with grid period (left), and measured FWHM for each grid period (right).
FIG. 7. 3D surface profile and height profile of protected silver mirror, by applying the HiLo optical imaging technique and searching for the brightest point along the z axis. Measured standard deviation of the height profile is 69 nm without curve fitting, and 17 nm with curve fitting.
FIG. 8. Results of 3D surface profile measurement and height profile of selected sample (a) by (b) LSCM and (c) HiLo; (d) height profiles from both techniques.
FIG. 9. Results for the tip of stationary knife: (a) surface, (b) WFM image, (c) HiLo image, (d) 3D surface profile measurement, (e) height profile presenting three different angles.
TABLE 1. Grating pattern and illuminated grid period on a sample surface
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