생명과학회지 (Journal of Life Science)

  • 제28권1호
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  • Pages.90-98
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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생균제의 부형제(운반체)로서의 난각분말의 특성

Characteristics of Eggshell Powder as Carriers of Probiotics

  • 이우도 (건국대학교 동물자원과학과) ;
  • 우개민 (건국대학교 동물자원과학과) ;
  • 임정민 (건국대학교 동물자원과학과) ;
  • 이권정 (건국대학교 동물자원과학과) ;
  • 이봉주 (국립수산과학원 사료연구센터) ;
  • 김강웅 (국립수산과학원 사료연구센터) ;
  • 김경덕 (국립수산과학원 사료연구센터) ;
  • 허상우 (국립수산과학원 사료연구센터) ;
  • 한현섭 (국립수산과학원 사료연구센터) ;
  • 김수기 (건국대학교 동물자원과학과)
  • Lee, Woo-Do (Department of Animal Science and Technology, Konkuk University) ;
  • Niu, Kai-Min (Department of Animal Science and Technology, Konkuk University) ;
  • Lim, Jeong-Min (Department of Animal Science and Technology, Konkuk University) ;
  • Yi, Kwon-Jung (Department of Animal Science and Technology, Konkuk University) ;
  • Lee, Bong-Joo (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Kim, Kang-Woong (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Kim, Kyoung-Duck (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Hur, Sang-Woo (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Han, Hyon-Sob (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Kim, Soo-Ki (Department of Animal Science and Technology, Konkuk University)
  • 투고 : 2018.01.08
  • 심사 : 2018.01.16
  • 발행 : 2018.01.30
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초록

계란 가공 부산물인 난각(ES: Eggshell)은 탄산칼슘 함량이 높아 사료에 첨가하여 칼슘원으로 이용되고 있다. 본 연구에서는 ES를 생균제의 부형제인 운반체로서 활용 가능성을 처음으로 시도하였다. L. plantarum을 대두박(SBM: Soybean meal), 난각조각(ESL: Eggshell powder with large particles), 난각미세분말(ESF: Eggshell powder with fine particles), 그리고 이들의 복합운반체인 SBM+ESL과 SBM+ESF에 생균제를 흡착시켜 그 부착상태를 주사전자현미경으로 확인하였다. 이 중 복합운반체인 SBM+ESF는 상온에서 4주 동안 pH 7~8을 유지하면서 L. plantarum의 가장 높은 생존율을 보였다. 본 연구에 사용한 모든 생균제들은 보존기간 동안 $4^{\circ}C$에서는 높은 생존율을 보였다. $30^{\circ}C$에서는 유산균수는 크게 감소하였으나, B. licheniformis는 높은 생존율을 유지하였고 B. subtilis, B. amyloliquefaciens와 S. cerevisiae는 $2{\log}_{10}$ (CFU/g)정도 감소하였다. 상기 연구결과는 사료의 칼슘원으로 이용되는 난각미세분말(ESF)을 대두박과 혼합하여 사용하면 B. licheniformis를 비롯한 일부 생균제의 생존성을 향상시켜 부형제(운반체)로도 사용할 수 있음을 밝혔다.

Eggshell (ES) is a by-product of table eggs with high content of calcium carbonate which can be used as a calcium source in feed. In this study, we have first illuminated the potential application of ES as a novel carrier for probiotics. The carriers used in the study include a SBM (Soybean meal), ESL (Eggshell powder with large particles), ESF (Eggshell powder with fine particles), and the complex carriers (SBM+ESL, SBM+ESF). The structure of carriers absorbed by L. plantarum was confirmed by SEM image. Among these carriers, the complex carrier SBM+ESF showed the highest viability of L. plantarum with pH 7~8 during four weeks storage at room temperature. The SBM+ESF was further tested as a carrier for various probiotic strains at $4^{\circ}C$ or $30^{\circ}C$. All the probiotic strains showed high viability at $4^{\circ}C$ storage. However, a significant reduction of Lactobacillus cells was observed at $30^{\circ}C$ storage. B. lichenifomis maintained high viability whereas B. subtilis, B. amyloliquefaciens, and S. cerevisiae showed the reduction of $2{\log}_{10}$ (CFU/g). These results suggest that if the ESF as a calcium source in feed was mixed with SBM, it can be used as an effective complex carrier for improving the viability of some probiotics including B. licheniformis.

키워드

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