생명과학회지 (Journal of Life Science)

  • 제28권1호
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  • Pages.26-36
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

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명월초, 여주 및 울금을 포함한 돼지감자 복합물의 항산화 및 AMPK 활성화를 통한 항당뇨 활성

Antioxidant and Antidiabetic Activities of Jerusalem Artichoke Composites Containing Gynura procumbens, Momordica charantia, and Curcuma longa via AMPK Activation

  • 이수정 (경상대학교 농업생명과학연구원) ;
  • 호문사 (경상대학교 식품영양학과) ;
  • 표재호 (산청기능성콩영농조합법인) ;
  • 류지현 (경상대학교 의과대학 생리학교실) ;
  • 강다원 (경상대학교 의과대학 생리학교실) ;
  • 정보영 (경상대학교 농업생명과학연구원) ;
  • 성낙주 (경상대학교 식품영양학과)
  • Lee, Soo-Jung (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Hu, Wen-Si (Department of Food Science and Nutrition, Gyeongsang National University) ;
  • Pyo, Jae-Ho (Sancheong of Functional Bean Farming Union Corporation) ;
  • Ryu, Ji Hyeon (Department of Physiology, College of Medicine, Gyeongsang National University) ;
  • Kang, Dawon (Department of Physiology, College of Medicine, Gyeongsang National University) ;
  • Jeong, Bo-Young (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Sung, Nak-Ju (Department of Food Science and Nutrition, Gyeongsang National University)
  • 투고 : 2017.08.22
  • 심사 : 2017.10.09
  • 발행 : 2018.01.30
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초록

명월초, 여주 및 울금의 물 및 70% 에탄올 추출물에서 항산화 및 ${\alpha}$-glucosidase 저해활성을 측정하였으며, 돼지감자 농축액에 이들 식물류의 에탄올 추출물을 첨가한 5종의 돼지감자 복합물(JA1~5)에서 항산화 및 항당뇨 활성을 비교하였다. 명월초, 여주 및 울금의 에탄올 추출물은 물 추출물에 비해 총 페놀 및 플라보노이드 함량이 많았다. DPPH, ABTS 라디칼 소거활성 및 FRAP법에 의한 환원력은 총 페놀 및 플라보노이드 함량에 의존적이었으며, 특히 명월초와 울금의 에탄올 추출물은 항산화 활성이 비슷한 수준이었다. ${\alpha}$-Glucosidase 저해활성은 에탄올 추출물의 $2,000{\mu}g/ml$ 농도에서 50% 이상이었다. 5종의 복합물은 돼지감자 농축액에 비해 총 페놀 및 플라보노이드 함량이 많았다. 또한 항산화 활성 및 ${\alpha}$-glucosidase 저해활성도 복합물에서 모두 증가되었으며, 특히 JA1의 활성이 유의적으로 높았다. 돼지감자 복합물은 인체 정상 간세포주인 Chang세포에 대해 세포독성이 없었으며, Chang세포에서 AMPK 활성화 및 근육세포인 C2C12세포에서 세포 내 당 유입을 유의적으로 증가시켰다. 이로써 명월초, 여주 및 울금의 추출물이 혼합된 돼지감자 복합물은 항산화 및 항당뇨 활성 증대에 효과적인 것으로 판단된다.

This study was performed to identify the antioxidant and ${\alpha}$-glucosidase inhibitory activities of water and 70% ethanol extracts of the three following herbs: G. procumbens, M. charantia, and C. longa. In addition, the antioxidant and antidiabetic activities of five types of Jerusalem artichoke composites (JA1 - 5), which were prepared by adding ethanol extracts of several herbs to Jerusalem artichoke concentrate, were studied and compared. The results showed that the total phenol and flavonoid contents of the ethanol extracts were higher than those of the water extracts. The DPPH and ABTS radical scavenging activities and reducing power depended on the total phenol and flavonoid contents. The antioxidant activities of ethanol extracts from G. procumbens and C. longa were comparable. Moreover, the ${\alpha}$-glucosidase inhibitory activity of the ethanol extracts ($2,000{\mu}g/ml$) from each herb was found to be over 50%. In contrast, the five types of JA composites showed higher total phenol and flavonoid contents than those of JA concentrate. In addition, increased antioxidant and ${\alpha}$-glucosidase inhibitory activities were observed, with that of JA1 being the highest. However, all concentrations ($1{\sim}100{\mu}g/ml$) of JA tested did not affect the cell viability of Chang cells. In addition, JA induced the activation of AMP-activated protein kinase (AMPK) in Chang cells and significantly increased the glucose uptake in C2C12 cells. Therefore, it could be concluded that the JA composites (JA1 - 5) mixed with G. procumbens, M. charantia, and C. longa extracts were effective in increasing the extracts' antioxidant and antidiabetic activities.

키워드

참고문헌

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