Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Antioxidant Activities and Hair-growth Promotion Effects of Tenebrio molitor Larvae Extracts (TMEs)

갈색거저리 유충 추출물의 항산화 활성 및 모발 성장 촉진 효과

  • Baek, Minhee (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seo, Minchul (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Mi-Ae (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yun, Eun-Young (Graduate School of Integrated Bioindustry, Sejong University) ;
  • Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
  • 백민희 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 서민철 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김미애 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 윤은영 (세종대학교 대학원 바이오산업융합학과) ;
  • 황재삼 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2017.09.12
  • Accepted : 2017.11.01
  • Published : 2017.11.30
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Abstract

Tenebrio molitor samples were investigated as novel biomaterials and sources of food in several recent studies. However, the insects' effects on hair growth were not sufficiently researched. To develop novel and natural materials for preventing alopecia and promoting hair growth, this study investigated the antioxidant activities and hair-growth promotion effects of TMEs. To determine the antioxidant activities, the TMEs' DPPH radical- and nitrite-scavenging activities were examined. To determine hair-growth promotion effects, proliferations of human dermal papilla cells (DPCs) and the murine fibroblast cell line NIH3T3 were evaluated by using an MTS assay. In addition, estimations were made for cell viabilities against cell death induced by dihydrotesterone (DHT) in DPCs and inhibitory effects against potassium channel blocking induced by tolbutamide (TBM) in NIH3T3 cells. The DPPH radical scavenging activity was 81.17%, and the nitrite scavenging activity was 43.69%; the activities were similar to the activities of blueberry extracts. Moreover, the TMEs promoted the proliferation of human DPCs and NIH3T3 cells, which were concentrated dependently. The TMEs prevented not only DHT-induced DPC cytotoxicity but also TBM's action as a potassium channel blocker in NIH3T3 cells. The results suggested that TME could be used as a functional therapeutic alopecia reagent, to prevent hair loss and to promote hair growth.

최근 들어 곤충을 식품 및 바이오 소재로 이용한 연구가 활발히 진행되고 있다. 그러나 곤충을 이용한 모발 성장 효과에 대한 연구는 아직 미흡한 실정이다. 따라서 본 연구에서는 탈모 예방 및 모발 성장 효과를 가진 새로운 천연물 소재 개발을 위해 갈색거저리 유충 추출물의 항산화 활성 및 모발 성장 촉진 효과를 연구하였다. 갈색거저리 유충 추출물의 항산화 활성 평가를 위해서 DPPH 라디칼 및 아질산염 소거능을 측정하였다. 모발 성장촉진 효과를 측정하기 위해서는 인간 모유두세포(human dermal papilla cell)와 섬유아세포(fibroblast, NIH3T3 cell)를 이용하였으며 MTS assay를 통해 세포생존율 및 세포증식률을 측정하였다. 모유두세포에서 dihydrotesteone (DHT)에 의한 세포사 억제 효과를 확인하였으며, 섬유아세포에서는 tolbutamide (TBM)의 potassium channel blocker 역할에 의한 세포사 억제 효과를 확인하였다. DPPH radical 및 아질산염 소거능 측정 결과 갈색거저리 유충 추출물은 항산화 역할이 뛰어난 것으로 보고된 블루베리와 유사하거나 높은 정도의 항산화능을 가지는 것으로 확인되었다. In vitro 상에서 갈색거저리 유충 추출물을 48시간 동안 처리한 경우, 모유두세포와 섬유아세포의 세포증식을 218% 및 116%까지 증가시켰다. 또한, 모유두세포에서 DHT 처리에 의한 세포사가 갈색거저리 유충 추출물에 의해 억제되는 것을 확인하였으며, 섬유아세포에서는 potassium channel blocker인 TBM에 의해 세포생존율이 감소하였으나 갈색거저리 유충 추출물 처리 시 세포생존율이 정상군과 비슷한 정도로 회복되는 것을 확인하였다. 이상의 결과로부터 갈색거저리 유충 추출물을 이용한 모발성장 및 탈모방지 기능성 소재 개발 가능성을 확인하였다.

Keywords

References

  1. Baek, M., Yoon, Y. I., Kim, M. A., Hwang, J. S., Goo, T. W. and Yun, E. Y. 2015. Physical and sensory evaluation of Tenebrio molitor larvae cooked by various cooking methods. Kor. J. Food Cook. Sci. 31, 534-543. https://doi.org/10.9724/kfcs.2015.31.5.534
  2. Bastide, N. M., Pierre, F. H. and Corpet, D. E. 2011. Heme iron from meat and risk of colorectal cancer: a meta-analysis and a review of the mechanisms involved. Cancer Prev. Res. 4, 177-184. https://doi.org/10.1158/1940-6207.CAPR-10-0113
  3. Brand-Williams, W., Cuvelier, M. E. and Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28, 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  4. Buhl, A. E., Waldon, D. J., Conrad, S. J., Mulholland, M. J., Shull, K. L., Kubicek, M. F., Johnson, G. A., Brunden, M. N., Stefanski, K. J. and Stehle, R. G. 1992. Potassium channel conductance: a mechanism affecting hair growth both in vitro and in vivo. J. Invest. Dermatol. 98, 315-319. https://doi.org/10.1111/1523-1747.ep12499788
  5. Chao, D., Bahl, P., Houlbrook, S., Hoy, L., Harris, A. and Austyn, J. M. 1999. Human cultured dendritic cells show differential sensitivity to chemotherapy agents as assessed by the MTS assay. Br. J. Cancer 81, 1280-1284. https://doi.org/10.1038/sj.bjc.6694366
  6. Choi, S. Y., Chung, M. J., Lee, S. J., Jung, H. S. and Sung, N. J. 2007. N-nitrosamine inhibition by strawberry, garlic, kale, and the effects of nitrite-scavenging and N-nitrosamine formation by functional compounds in strawberry and garlic. Food Control 18, 485-491. https://doi.org/10.1016/j.foodcont.2005.12.006
  7. Chung, M. Y., Kwon, E. Y., Hwang, J. S., Goo, T. W. and Yun, E. Y. 2013. Pre-treatment conditions on the powder of Tenebrio molitor for using as a novel food ingredient. J. Seric. Entomol. Sci. 51, 9-14.
  8. Cotelle, P. and Vezin, H. 2011. Reaction of caffeic acid and derivatives with acidic nitrite. Tetrahedron Lett. 42, 3303-3305.
  9. Deborah, A. S., Ian, F., Darren, M. T., Michael, P. P., Gillian, E. W. and Terence, K. 1996. In the absence of streptomycin, minoxidil potentiates the mitogenic effects of fetal calf serum, insulin-like growth factor 1, and platelet-derived growth factor on NIH3T3 fibroblasts in a $K^{+}$ channel-dependent fashion. J. Invest. Dermatol. 107, 229-234. https://doi.org/10.1111/1523-1747.ep12329697
  10. Gray, J. I. and Dugan, L. R. J. 1975. Inhibition of N-nitrosamine formation in model food systems. J. Food Sci. 40, 981-984. https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  11. Hagemann, T., Schlutter-Bohmer, B., Allam, J. P., Bieber, T. and Novak, N. 2005. Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution. Contact Dermatitis 53, 53-55. https://doi.org/10.1111/j.0105-1873.2005.00456b.x
  12. Hwang, H. S., Hwang, T. H., Pyo, A. H. and Ju, E. H. 2017. Anti-oxidant efficacy and effects on expression of growth factors in human hair follicle dermal papilla cells of Rosa multiflora root extracts. Asian J. Beauty Cosmetol. 15, 146-158. https://doi.org/10.20402/ajbc.2016.0109
  13. Hwang, S. Y., Bae, G. and Choi, S. K. 2015. Preferences and purchase intention of Tenebrio molitor (Mealworm) according to cooking method. Kor. J. Culin. Res. 21, 100-115.
  14. Jeon, H. Y., Kim, S. H., Kim, C. W., Shin, H. J., Seo, D. B. and Lee, S. J. 2011. Hair growth promoting effect of black soybean extract in vitro and in vivo. Kor. J. Food Sci. Technol. 43, 747-753. https://doi.org/10.9721/KJFST.2011.43.6.747
  15. Jo, S. J., Choi, S. J., Yoon, S. Y., Lee, J. Y., Park, W. S., Park, P. J., Kim, K. H., Eun, H. C. and Kwon, O. 2013. Valproic acid promotes human hair growth in in vitro culture model. J. Dermatol. Sci. 72, 16-24. https://doi.org/10.1016/j.jdermsci.2013.05.007
  16. Joo, S. S. 2011. In vitro and In vivo Hair growth promotion effects of Lactobacillus plantarum-fermented plant extracts (MBN). Kor. J. Food Sci. Technol. 43, 381-386. https://doi.org/10.9721/KJFST.2011.43.3.381
  17. Kang, K. I., Kim, S. C., Kim, M. K., Boo, H. J., Jeon, Y. J., Koh, Y. S., Yoo, E. S., Kang, S. M. and Kang, H. K. 2012. Effect of dieckol, a component of Ecklonia cava, on the promotion of hair growth. Int. J. Mol. Sci. 13, 6407-6423. https://doi.org/10.3390/ijms13056407
  18. Kim, S. H., Shon, J. Y., Park, J. S., Kim, J. W., Kang, J. H., Yun, E. Y., Hwang, J. S. and Kim, H. M. 2016. Change in dietary intake and nutritional status using mealworms as hospital meal in postoperative patients. J. Kor. Diet. Assoc. 22, 292-309.
  19. Kim, Y., Han, H. and Park, Y. 2015. The plan for activation of insect industry. Korea Rural Economic Institute R758, 1-148.
  20. Lee, H. S. and Hwang, S. Y. 2009. Effects of $DCS^{(R)}$ hair tonic on hair growth promotion in an alopecia model of C57BL/6 mice. Kor. Education J. Aesthet. Soc. 7, 131-142.
  21. Lee, J. E., Lee, A. J., Jo, D. E., Cho, J. H., Youn, K., Yun, E. Y., Hwang, J. S., Jun, M. and Kang, B. H. 2015. Cytotoxicity effects of Tenebrio molitor larval extracts against hepatocellular carcinoma. J. Kor. Soc. Food Sci. Nutr. 44, 200-207. https://doi.org/10.3746/jkfn.2015.44.2.200
  22. Lee, S. G., Yu, M. H., Lee, S. P. and Lee, I. S. 2008. Antioxidant activities and induction of apoptosis by methanol extracts from avocado. J. Kor. Soc. Food Sci. Nutr. 37, 269-275. https://doi.org/10.3746/jkfn.2008.37.3.269
  23. Liu, J., Kurashiki, K., Shimizu, K. and Kondo, R. 2006. 5alpha-reductase inhibitory effect of triterpenoids isolated from Ganoderma lucidum. Biol. Pharm. Bull. 29, 392-395. https://doi.org/10.1248/bpb.29.392
  24. McClellan, K. J. and Markham, A. 1999. Finasteride: A review of its use in male pattern hair loss. Drugs 57, 111-126. https://doi.org/10.2165/00003495-199957010-00014
  25. Moon, H. K., Lee, S. W. and Kim, J. K. 2013. Physicochemical and quality characteristics of the Korean and American blueberries. Kor. J. Food Preserv. 20, 524-531. https://doi.org/10.11002/kjfp.2013.20.4.524
  26. Morgan, M. B. and Rose, P. 2003. An investigation of apoptosis in androgenetic alopecia. Ann. Clin. Lab. Sci. 33, 107-112.
  27. Park, G. H., Park, K. Y., Cho, H. I., Lee, S. M., Han, J. S., Won, C. H., Chang, S. E., Lee, M. W., Choi, J. H., Moon, K. C., Shin, H., Kang, Y. J. and Lee, D. H. 2015. Red ginseng extract promotes the hair growth in cultured human hair follicles. J. Med. Food 18, 354-362. https://doi.org/10.1089/jmf.2013.3031
  28. Park, S. A., Ko, K. S., In, M. H., Mun, Y. J. and Woo, W. H. 2017. Effect of Puerariae radix ethanol extract on the proliferation of human dermal papilla cells. J. Physiol. Pathol. Kor. Med. 31, 167-172. https://doi.org/10.15188/kjopp.2017.06.31.3.167
  29. Park, W. S., Sung, D. S., Kim, D. K., Cho, W. H., Lee, H. K., Lee, C. H., Park, S. K. and Sim, Y. C. 2004. The effect of hair essence (HHRHG0202-80) containing five herbal extracts on hair growth and the prevention of alopecia in vitro & vivo. J. Kor. Orient. Med. 24, 152-160.
  30. Park, Y. M. and Han, J. S. 2016. A study on the utilization of Dendropanax morbifera Lev. leaf extract for material of functional cosmetics and hair growth products. Asian J. Beauty Cosmetol. 14, 277-288. https://doi.org/10.20402/ajbc.2016.0051
  31. Rastegar, H., Ahmadi Ashtiani, H., Aghaei, M., Ehsani, A. and Barikbin, B. 2013. Combination of herbal extracts and platelet-rich plasma induced dermal papilla cell proliferation: involvement of ERK and Akt pathways. J. Cosmet. Dermatol. 12, 116-122. https://doi.org/10.1111/jocd.12033
  32. Rastegar, H., Ashtiani, H. A., Aghaei, M., Barikbin, B. and Ehsani, A. 2015. Herbal extracts induce dermal papilla cell proliferation of human hair follicles. Ann. Dermatol. 27, 667-675. https://doi.org/10.5021/ad.2015.27.6.667
  33. Roh, S. S., Kim, C. D., Lee, M. H., Hwang, S. L., Rang, M. J. and Yoon, Y. K. 2002. The hair growth promoting effect of Sophora flavescens extract and its molecular regulation. J. Dermatol. Sci. 30, 43-49. https://doi.org/10.1016/S0923-1811(02)00060-9
  34. Schneider, M. R., Schmidt-Ullrich, R. and Paus, R. 2009. The hair follicle as a dynamic miniorgan. Curr. Biol. 19, R132-142. https://doi.org/10.1016/j.cub.2008.12.005
  35. Sebranek, J. G. and Bacus, J. N. 2007. Cured meat products without direct addition of nitrate or nitrite: what are the issues? Meat Sci. 77, 136-147. https://doi.org/10.1016/j.meatsci.2007.03.025
  36. Seo, M., Goo, T. W., Chung, M. Y., Baek, M., Hwang, J. S., Kim, M. A. and Yun, E. Y. 2017. Tenebrio molitor larvae inhibit adipogenesis through AMPK and MAPKs signaling in 3T3-L1 adipocytes and obesity in high-fat diet-induced obese mice. Int. J. Mol. Sci. 18, 518. https://doi.org/10.3390/ijms18030518
  37. Seo, M., Kim, J., Moon, S. S., Hwang, J. S. and Kim, M. A. 2017. Intraventricular administration of Tenebrio molitor larvae extract regulates food intake and body weight in mice with high-fat diet-induced obesity. Nutr. Res. 44, 18-26. https://doi.org/10.1016/j.nutres.2017.05.011
  38. Tilak, J. C., Banerjee, M., Mehan, H. and Devasagayam, T. P. A. 2004. Antioxidant availiability of tumeric in relation to its medicinal and culinary uses. Phytother. Res. 18, 798-804. https://doi.org/10.1002/ptr.1553
  39. Youn, K., Yun, E. Y., Lee, J., Kim, J. Y., Hwang, J. S., Jeong, W. S. and Jun, M. 2014. Oleic acid and linoleic acid from Tenebrio molitor larvae inhibit BACE1 activity in vitro: Molecular docking studies. J. Med. Food 17, 284-289. https://doi.org/10.1089/jmf.2013.2968
  40. Yun, E. Y. and Hwang, J. S. 2016. Status and prospect for development of insect foods. Food Sci. Ind. 49, 31-39.