Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Hepatoprotective Effects of Hep G2 Cells and the Alcohol-Metabolizing Enzyme Activities of Lemon-Myrtle (Backhousia citriodora) Leaf Extracts

레몬 머틀 잎 추출물의 Hep G2 세포에서의 간 보호 효과 및 알코올대사 효소활성

  • Jung, Kyung Im (Department of Food & Nutrition, Silla University) ;
  • Kim, Pan Kil (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Gal, Sang Wan (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Choi, Young Ju (Department of Food & Nutrition, Silla University)
  • 정경임 (신라대학교 식품영양학과) ;
  • 김판길 (경남과학기술대학교 제약공학과) ;
  • 갈상완 (경남과학기술대학교 제약공학과) ;
  • 최영주 (신라대학교 식품영양학과)
  • Received : 2017.08.25
  • Accepted : 2017.10.27
  • Published : 2017.11.30
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Abstract

Lemon myrtle (Backhousia citriodora), a plant in the Myrtaceae family, is native to the semitropical rain-forests of Queensland and is presumably the most commercialized native spice. In Australian thousands of lemon-myrtle trees are under tillage. This study was carried out to investigate the alcohol metabolism, hepatoprotective effects and antidiabetic, tyrosinase inhibitory activity of hot-water (LMW) and 80% ethanol (LME) extracts from lemon-myrtle leaves. The alpha-glucosidase (${\alpha}$-glucosidase) inhibitory activities of the LMW and LME extracts were 7.66% and 40.29% at 1 mg/ml (p<0.05), respectively. The tyrosinase inhibitory activity of the LME extract was about 38.26 % at 1 mg/ml. The effects the LMW and LME extracts had on alcohol-metabolizing activities were determined by measuring the generation of reduced nicotinamide-adenine dinucleotide (NADH) by acetaldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH). The ADH activities of the LMW and LME extracts significantly increased in a dose-dependent manner and were about 154.40% and 192.03% at 1 mg/ml, respectively (p<0.05). The ALDH activities of the LMW and LME extracts also significantly increased in a dose-dependent manner and were about 151.14% and 192.34% at 1 mg/ml, respectively (p<0.05). At $100{\mu}g/ml$, the LMW and LME extracts showed significant protective effects against tacrine-induced cytotoxicity in Hep G2 cells. The results suggested that Backhousia citriodora leaf extracts have the potential to be significant sources for natural health products.

본 연구에서는 레몬 머틀 잎 열수 및 에탄올 추출물의 새로운 소재로서의 가능성을 검토하고자 항당뇨 효과와 미백 효과, 알코올 분해능 및 간세포 보호 효과를 확인하였다. 레몬 머틀 추출물의 혈당강하효과는 ${\alpha}$-glucosidase 활성 억제능을 측정하였으며, 열수 및 에탄올 추출물 1 mg/ml 농도에서 각각 7.66%와 40.29%로 에탄올 추출물이 높게 나타났고(p<0.05), tyrosinase 저해활성은 에탄올 추출물 1 mg/ml 농도에서 38.26%로 나타났다. 숙취 해소능을 알아보기 위해 alcohol dehydrogenase (ADH) 및 acetaldehyde dehydrogenase (ALDH) 활성을 측정한 결과, 두 효소 모두 레몬 머틀 열수 및 에탄올 추출물의 농도 의존적으로 증가하였다(p<0.05). 레몬 머틀 열수 및 에탄올 추출물은 tacrine으로 유도된 Hep G2 간암 세포주에 대하여 유의적인 보호 활성이 나타났다. 이상의 결과에서와 같이 레몬 머틀 잎은 항당뇨효과와 숙취 해소효과 및 간세포 보호 효과가 있는 것으로 나타났기에 기능성 소재로서의 활용 가능성을 확인할 수 있었다.

Keywords

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