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Optimal Temperature and Light Intensity for Improved Mixotrophic Metabolism of Chlorella sorokiniana Treating Livestock Wastewater

  • Lee, Tae-Hun (Department of Environmental Engineering and Soil Environment Research Center, Chonbuk National University) ;
  • Jang, Jae Kyung (Energy and Environmental Engineering Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Hyun-Woo (Department of Environmental Engineering and Soil Environment Research Center, Chonbuk National University)
  • Received : 2017.07.06
  • Accepted : 2017.09.02
  • Published : 2017.11.28

Abstract

Mixotrophic microalgal growth gives a great premise for wastewater treatment based on photoautotrophic nutrient utilization and heterotrophic organic removal while producing renewable biomass. There remains a need for a control strategy to enrich them in a photobioreactor. This study performed a series of batch experiments using a mixotroph, Chlorella sorokiniana, to characterize optimal guidelines of mixotrophic growth based on a statistical design of the experiment. Using a central composite design, this study evaluated how temperature and light irradiance are associated with $CO_2$ capture and organic carbon respiration through biomass production and ammonia removal kinetics. By conducting regressions on the experimental data, response surfaces were created to suggest proper ranges of temperature and light irradiance that mixotrophs can beneficially use as two types of energy sources. The results identified that efficient mixotrophic metabolism of Chlorella sorokiniana for organics and inorganics occurs at the temperature of $30-40^{\circ}C$ and diurnal light condition of $150-200{\mu}mol\;E{\cdot}m^{-2}{\cdot}s^{-1}$. The optimal specific growth rate and ammonia removal rate were recorded as 0.51/d and 0.56/h on average, respectively, and the confirmation test verified that the organic removal rate was $105mg\;COD{\cdot}l^{-1}{\cdot}d^{-1}$. These results support the development of a viable option for sustainable treatment and effluent quality management of problematic livestock wastewater.

Keywords

References

  1. Zhang H, Wang W, Li Y, Yang W, Shen G. 2011. Mixotrophic cultivation of Botryococcus braunii. Biomass Bioenergy 35:1710-1715. https://doi.org/10.1016/j.biombioe.2011.01.002
  2. Deschênes J-S, Boudreau A, Tremblay R. 2015. Mixotrophic production of microalgae in pilot-scale photobioreactors:practicability and process considerations. Algal Res. 10: 80-86. https://doi.org/10.1016/j.algal.2015.04.015
  3. Ji M-K, Abou-Shanab RAI, Kim S-H, Salama E-S, Lee S-H, Kabra AN, et al. 2013. Cultivation of microalgae species in tertiary municipal wastewater supplemented with $CO_2$ for nutrient removal and biomass production. Ecol. Eng. 58:142-148. https://doi.org/10.1016/j.ecoleng.2013.06.020
  4. Li T, Zheng Y, Yu L, Chen S. 2014. Mixotrophic cultivation of a Chlorella sorokiniana strain for enhanced biomass and lipid production. Biomass Bioenergy 66: 204-213. https://doi.org/10.1016/j.biombioe.2014.04.010
  5. Tang C-C, Zuo W, Tian Y, Sun N, Wang Z-W, Zhang J. 2016. Effect of aeration rate on performance and stability of algal-bacterial symbiosis system to treat domestic wastewater in sequencing batch reactors. Bioresour. Technol. 222: 156-164. https://doi.org/10.1016/j.biortech.2016.09.123
  6. Ji MK, Yun HS, Park YT, Kabra AN, Oh IH, Choi J. 2015. Mixotrophic cultivation of a microalga Scenedesmus obliquus in municipal wastewater supplemented with food wastewater and flue gas $CO_2$ for biomass production. J. Environ. Manag. 159: 115-120. https://doi.org/10.1016/j.jenvman.2015.05.037
  7. Ceron Garcia MC, Camacho FG, Miron AS, Sevilla JMF, Chisti Y, Grima EM. 2006. Mixotrophic production of marine microalga Phaeodactylum tricornutum on various carbon sources. J. Microbiol. Biotechnol. 16: 689-694.
  8. Ji MK, Kim HC, Sapireddy VR, Yun HS, Abou-Shanab RA, Choi J, et al. 2013. Simultaneous nutrient removal and lipid production from pretreated piggery wastewater by Chlorella vulgaris YSW-04. Appl. Microbiol. Biotechnol. 97: 2701-2710. https://doi.org/10.1007/s00253-012-4097-x
  9. Ling J , Nip S, Cheok WL, de Toledo RA, Shim H. 2014. Lipid production by a mixed culture of oleaginous yeast and microalga from distillery and domestic mixed wastewater. Bioresour. Technol. 173: 132-139. https://doi.org/10.1016/j.biortech.2014.09.047
  10. Zhang H, Wang W, Li Y, Yang W, Shen G. 2011. Mixotrophic cultivation of Botryococcus braunii. Biomass Bioenergy 35: 1710-1715. https://doi.org/10.1016/j.biombioe.2011.01.002
  11. Amaro HM, Guedes AC, Malcata FX. 2011. Advances and perspectives in using microalgae to produce biodiesel. Appl. Energy 88: 3402-3410. https://doi.org/10.1016/j.apenergy.2010.12.014
  12. Goncalves AL, Simoes M, Pires JCM. 2014. The effect of light supply on microalgal growth, $CO_2$ uptake and nutrient removal from wastewater. Energy Convers. Manag. 85: 530-536. https://doi.org/10.1016/j.enconman.2014.05.085
  13. Bhatnagar A, Chinnasamy S, Singh M, Das KC. 2011. Renewable biomass production by mixotrophic algae in the presence of various carbon sources and wastewaters. Appl. Energy 88: 3425-3431. https://doi.org/10.1016/j.apenergy.2010.12.064
  14. Lin TS, Wu JY. 2015. Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition. Bioresour. Technol. 184: 100-107. https://doi.org/10.1016/j.biortech.2014.11.005
  15. Das P, Lei W, Aziz SS, Obbard JP. 2011. Enhanced algae growth in both phototrophic and mixotrophic culture under blue light. Bioresour. Technol. 102: 3883-3887. https://doi.org/10.1016/j.biortech.2010.11.102
  16. Mahapatra DM, Chanakya HN, Ramachandra TV. 2014. Bioremediation and lipid synthesis through mixotrophic algal consortia in municipal wastewater. Bioresour. Technol. 168: 142-150. https://doi.org/10.1016/j.biortech.2014.03.130
  17. Kim HW, Vannela R, Zhou C, Harto C, Rittmann BE. 2010. Photoautotrophic nutrient utilization and limitation during semi-continuous growth of Synechocystis sp. PCC6803. Biotechnol. Bioeng. 106: 553-563. https://doi.org/10.1002/bit.22724
  18. Montgomery DC. 2017. Design and Analysis of Experiments. John Wiley & Sons, New York.
  19. Burton FL, Stensel HD, Tchobanoglous G. 2014. Wastewater engineering: treatment and Resource Recovery. McGraw-Hill, New York.
  20. Yeh K-L, Chang J-S, Chen W-M. 2010. Effect of light supply and carbon source on cell growth and cellular composition of a newly isolated microalga Chlorella vulgaris ESP-31. Eng. Life Sci. 10: 201-208. https://doi.org/10.1002/elsc.200900116
  21. Kumar K, Dasgupta CN, Das D. 2014. Cell growth kinetics of Chlorella sorokiniana and nutritional values of its biomass. Bioresour. Technol. 167: 358-366. https://doi.org/10.1016/j.biortech.2014.05.118
  22. Derringer G. 1980. Simultaneous optimization of several response variables. J. Qual. Technol. 12: 214-219. https://doi.org/10.1080/00224065.1980.11980968
  23. Eaton AD, Clesceri LS, Rice EW, Greenberg AE, Franson MAH. 2014. Standard Methods for the Examination of Water and Wastewater, 2014. American Public Health Association, Washington, DC.
  24. Kim H-W, Park S, Rittmann BE. 2015. Multi-component kinetics for the growth of the cyanobacterium Synechocystis sp. PCC6803. Environ. Eng. Res. 20: 347-355. https://doi.org/10.4491/eer.2015.033
  25. Cordero BF, Obraztsova I, Couso I, Leon R, Vargas MA, Rodriguez H. 2011. Enhancement of lutein production in Chlorella sorokiniana (Chlorophyta) by improvement of culture conditions and random mutagenesis. Marine Drugs 9: 1607. https://doi.org/10.3390/md9091607
  26. Herrero A, Muro-Pastor AM, Flores E. 2001. Nitrogen control in cyanobacteria. J. Bacteriol. 183: 411-425. https://doi.org/10.1128/JB.183.2.411-425.2001
  27. Ceron Garcia MC, Sanchez Miron A, Fernandez Sevilla JM, Molina Grima E, Garcia Camacho F. 2005. Mixotrophic growth of the microalga Phaeodactylum tricornutum. Process Biochem. 40: 297-305. https://doi.org/10.1016/j.procbio.2004.01.016
  28. Madigan MT, Clark DP, Stahl D, Martinko JM. 2010. Brock Biology of Microorganisms, 13th Ed. Benjamin Cummings, San Francisco, CA.
  29. Ramirez-Verduzco LF, Rodriguez-Rodriguez JE, Jaramillo-Jacob AdR. 2012. Predicting cetane number, kinematic viscosity, density and higher heating value of biodiesel from its fatty acid methyl ester composition. Fuel 91: 102-111. https://doi.org/10.1016/j.fuel.2011.06.070
  30. Kim S, Park JE, Cho YB, Hwang SJ. 2013. Growth rate, organic carbon and nutrient removal rates of Chlorella sorokiniana in autotrophic, heterotrophic and mixotrophic conditions. Bioresour. Technol. 144: 8-13. https://doi.org/10.1016/j.biortech.2013.06.068
  31. Kumar K, Das D. 2012. Growth characteristics of Chlorella sorokiniana in airlift and bubble column photobioreactors. Bioresour. Technol. 116: 307-313. https://doi.org/10.1016/j.biortech.2012.03.074

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