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The relationship between the variants in the 5'-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels

  • Liu, ShuQin (Equine Center, China Agricultural University) ;
  • Lian, Song (College of Animal Science and Technology, China Agricultural University) ;
  • Yang, YunZhou (Equine Center, China Agricultural University) ;
  • Fu, ChunZheng (Equine Center, China Agricultural University) ;
  • Ma, HongYing (Equine Center, China Agricultural University) ;
  • Xiong, ZhiYao (Equine Center, China Agricultural University) ;
  • Ling, Yao (College of Animal Science and Technology, China Agricultural University) ;
  • Zhao, ChunJiang (Equine Center, China Agricultural University)
  • 투고 : 2017.02.15
  • 심사 : 2017.03.30
  • 발행 : 2017.12.01

초록

Objective: An experiment was conducted to study the association between the single nucleotide polymorphisms (SNPs) in 5'-untranslated regions (5'-UTR) of equine chorionic gonadotropin (eCG) genes and the serum eCG levels. Methods: SNPs in 5'-UTR of eCG genes were screened across 10 horse breeds, including 7 Chinese indigenous breeds and 3 imported breeds using iPLEX chemistry, and the association between the serum eCG levels of 174 pregnant Da'an mares and their serum eCG levels (determined with ELISA) was analyzed. Results: Four SNPs were identified in the 5'-UTR of the $eCG{\alpha}$ gene, and one of them was unique in the indigenous breeds. There were 2 SNPs detected at the 5' end of the $eCG{\beta}$ subunit gene, and one of them was only found in the Chinese breeds. The SNP g.39948246T>C at the 5'-UTR of $eCG{\alpha}$ was associated significantly with eCG levels of 75-day pregnant mare serum (p<0.05) in Da'an mares. Prediction analysis on binding sites of transcription factors showed that the g.39948246T>C mutation causes appearance of the specific binding site of hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which is a transcriptional repressor belonging to the forkhead protein family of transcription factors. Conclusion: The SNP g.39948246T>C at the 5'-UTR of $eCG{\alpha}$ is associated with eCG levels of 75-day pregnant mare serum (p<0.05).

키워드

참고문헌

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