Mycobiology

The Korean Society of Mycology (한국균학회)
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Diversity, Phylogeny, and Host-Specialization of Hyaloperonospora Species in Korea

  • Lee, Jae Sung (Department of Biology, Kunsan National University) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology & Agrochemistry, Chonnam National University) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University) ;
  • Choi, Young-Joon (Department of Biology, Kunsan National University)
  • Received : 2017.07.03
  • Accepted : 2017.09.04
  • Published : 2017.09.01
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Abstract

The genus Hyaloperonospora (Peronosporaceae; Oomycota) is an obligate biotrophic group that causes downy mildew disease on the Brassicaceae and allied families of Brassicales, including many economically relevant crops, such as broccoli, cabbage, radish, rape, and wasabi. To investigate the diversity of Hyaloperonospora species in northeast Asia, we performed a morphological analysis for the dried herbarium specimens collected in Korea, along with molecular phylogenetic inferences based on internal transcribed spacer rDNA and cox2 mtDNA sequences. It was confirmed that 14 species of Hyaloperonospora exist in Korea. Of these, three species, previously classified under the genus Peronospora, were combined to Hyaloperonospora: H. arabidis-glabrae comb. nov. (ex Arabis glabra), H. nasturtii-montani comb. nov. (ex Rorippa indica), and H. nasturtii-palustris comb. nov. (ex Rorippa palustris). In addition, finding two potentially new species specific to northeast Asian plants is noteworthy in support of the view that the species abundance of Hyaloperonospora has been underestimated hitherto.

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References

  1. Thines M, Choi YJ. Evolution, diversity, and taxonomy of the Peronosporaceae, with focus on the genus Peronospora. Phytopathology 2016;106:6-18. https://doi.org/10.1094/PHYTO-05-15-0127-RVW
  2. Constantinescu O. An annotated list of Peronospora names. Thunbergia 1991;15:1-110.
  3. Gaumann EA. Uber die formen der Peronospora parasitica (Pers.) Fries. Ein beitrag zur speziesfrage bei den parasitischen pilzen. Beih Bot Centralbl 1918;35:395-533.
  4. Gaumann EA. Beitrage zu einer Monographie der Gattung Peronospora Corda. Beit Kryptogamenfl Schweiz 1923;5:1-360.
  5. Constantinescu O, Fatehi J. Peronospora-like fungi (Chromista, Peronosporales) parasitic on Brassicaceae and related hosts. Nova Hedwigia 2002;74:291-338. https://doi.org/10.1127/0029-5035/2002/0074-0291
  6. Goker M, Riethmuller A, Voglmayr H, Weiss M, Oberwinkler F. Phylogeny of Hyaloperonospora based on nuclear ribosomal internal transcribed spacer sequences. Mycol Prog 2004;3:83-94. https://doi.org/10.1007/s11557-006-0079-7
  7. Goker M, Voglmayr H, Garcia-Blazquez G, Oberwinkler F. Species delimitation in downy mildews: the case of Hyaloperonospora in the light of nuclear ribosomal ITS and LSU sequences. Mycol Res 2009;113(Pt 3):308-25. https://doi.org/10.1016/j.mycres.2008.11.006
  8. Goker M, Voglmayr H, Riethmuller A, Weiss M, Oberwinkler F. Taxonomic aspects of Peronosporaceae inferred from Bayesian molecular phylogenetics. Can J Bot 2003;81:672-83. https://doi.org/10.1139/b03-066
  9. Riethmuller A, Voglmayr H, Goker M, Weiss M, Oberwinkler F. Phylogenetic relationships of the downy mildews (Peronosporales) and related groups based on nuclear large subunit ribosomal DNA sequences. Mycologia 2002;94:834-49. https://doi.org/10.1080/15572536.2003.11833177
  10. Voglmayr H. Phylogenetic relationships of Peronospora and related genera based on nuclear ribosomal ITS sequences. Mycol Res 2003;107(Pt 10):1132-42. https://doi.org/10.1017/S0953756203008438
  11. Choi YJ, Shin HD, Voglmayr H. Reclassification of two Peronospora species parasitic on Draba in Hyaloperonospora based on morphological and molecular phylogenetic data. Mycopathologia 2011;171:151-9. https://doi.org/10.1007/s11046-010-9340-3
  12. Voglmayr H, Choi YJ, Shin HD. Multigene phylogeny, taxonomy and reclassification of Hyaloperonospora on Cardamine. Mycol Prog 2014;13:131-44. https://doi.org/10.1007/s11557-013-0900-z
  13. Yerkes WD, Shaw CG. Taxonomy of the Peronospora species on Cruciferae and Chenopodiaceae. Phytopathology 1959;49:499-507.
  14. Cho WD, Shin HD. List of plant diseases in Korea. 4th ed. Suwon: Korean Society of Plant Pathology; 2004.
  15. Hong SY, Jee HJ, Choi YJ, Shin HD. First confirmed report of downy mildew caused by Hyaloperonospora parasitica on broccoli in Korea. Plant Pathol 2008;57:777.
  16. Choi YJ, Kim JY, Park JH, Shin HD. First report of tatsoi downy mildew caused by Hyaloperonospora brassicae in Korea. Plant Dis 2012;96:1703.
  17. Choi YJ, Mulenko W, Park JH, Shin HD. First report of downy mildew of spider flower caused by a Hyaloperonospora sp. in Korea. Plant Dis 2012;96:588.
  18. Choi YJ, Hong SB, Shin HD. Diversity of the Hyaloperonospora parasitica complex from core brassicaceous hosts based on ITS rDNA sequences. Mycol Res 2003;107:1314-22. https://doi.org/10.1017/S0953756203008578
  19. Shin HD, Choi YJ. Peronosporaceae of Korea. Suwon: National Institute of Agricultural Science and Technology; 2006.
  20. Nakata K, Takimoto K. List of diseases of cultivated plants in Korea. Bull Chosen Agric Exp Stn 1928;15:1-140.
  21. Shin HD, Kim WB. Fungal diseases of Capsella bursa-pastoris in Korea. Korean J Plant Resour 1997;10:360-8.
  22. Choi YJ, Park MJ, Kim JY, Shin HD. An unnamed Hyaloperonospora sp. causing downy mildew on arugula (rocket) in Korea. Plant Pathol 2010;59:1165.
  23. Choi YJ, Thines M, Choi IY, Shin HD. Perofascia is not monotypic: the description of the second taxon affecting the South American crop maca (Lepidium meyenii). Mycol Prog 2017;16:857-64. https://doi.org/10.1007/s11557-017-1320-2
  24. Bachofer M. Molekularbiologische Populationsstudien an Plasmopara halstedii, dem Falschen Mehltau der Sonnenblume. Stuttgart: University of Hohenheim; 2004.
  25. Moncalvo JM, Wang HH, Hseu RS. Phylogenetic relationships in Ganoderma Inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. Mycologia 1995;87:223-38. https://doi.org/10.1080/00275514.1995.12026524
  26. Hudspeth DS, Nadler SA, Hudspeth ME. A cox2 molecular phylogeny of the Peronosporomycetes. Mycologia 2000;92:674-84. https://doi.org/10.1080/00275514.2000.12061208
  27. Choi YJ, Beakes G, Glockling S, Kruse J, Nam B, Nigrelli L, Ploch S, Shin HD, Shivas RG, Telle S, et al. Towards a universal barcode of oomycetes: a comparison of the cox1 and cox2 loci. Mol Ecol Resour 2015;15:1275-88. https://doi.org/10.1111/1755-0998.12398
  28. Choi YJ, Klosterman SJ, Kummer V, Voglmayr H, Shin HD, Thines M. Multi-locus tree and species tree approaches toward resolving a complex clade of downy mildews (Straminipila, Oomycota), including pathogens of beet and spinach. Mol Phylogenet Evol 2015;86:24-34. https://doi.org/10.1016/j.ympev.2015.03.003
  29. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Mol Biol Evol 2013;30:772-80. https://doi.org/10.1093/molbev/mst010
  30. Katoh K, Toh H. Improved accuracy of multiple ncRNA alignment by incorporating structural information into a MAFFT-based framework. BMC Bioinformatics 2008;9:212. https://doi.org/10.1186/1471-2105-9-212
  31. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  32. Stamatakis A. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 2006;22:2688-90. https://doi.org/10.1093/bioinformatics/btl446
  33. Ito S, Tokunaga Y. Notae mycologicae Asiae orientalis. I. Trans Sapporo Nat Hist Soc 1935;14: 11-33.
  34. Gustavsson A. Studies on nordic peronosporas. I. Taxonomic revision. Opera Bot 1959;3:1-271.
  35. Hansford CG. Host list of the parasitic fungi of Uganda. Part I. East Afr Agric J 1943;8:248-52.
  36. Chardon CE. Adiciones a la Flora Micologica de Venezuela. Bol Soc Venez Cienc Nat 1939;40:325-68.
  37. Yin G, Yang Z. Peronospora. In: Yu Y, editor. Flora fungorum sinicorum. Vol. 6. Peronosporales. Beijing: Science Press; 1998. p. 247-328.
  38. Voglmayr H, Goker M. Morphology and phylogeny of Hyaloperonospora erophilae and H. praecox sp. nov., two downy mildew species co-occurring on Draba verna sensu lato. Mycol Prog 2011;10:283-92. https://doi.org/10.1007/s11557-010-0699-9
  39. Zhou T, Lu L, Ynag G, Al-Shehbaz IA. Brassicaceae (Cruciferae). In: Wu ZY, Raven PH, editors. Flora of China. Beijing: Science Press; 2001. p. 1-193.
  40. Al-Shehbaz IA, Arai K, Ohba H. Cardamine. In: Iwatsuki K, Boufford D, Ohba H, editors. Flora of Japan. Tokyo: Kodansha Ltd.; 2006. p. 482-90.
  41. Lee YN. Flora of Korea. Seoul: Kyo-Hak Publishing Co.; 2002.
  42. Berkutenko AN. Rod 21. Serdechnik - Cardamine L. In: Kharkevich SS, editor. Sosudistye rasteniya sovetskogo Dal'nego Vostoka. Vol. 3. Leningrad: Izdatel'stvo Nauka; 1988. p. 65-77.
  43. Lee WT. Coloured standard illustrations of Korean plants. Seoul: Academy; 1996.
  44. Shin SL, Lim YK, Kwon HJ, Kim YR, Kim SY. Morphological characteristics and germination conditions of seeds in Arabis pendula L. Korean J Plant Resour 2017;30:50-7. https://doi.org/10.7732/kjpr.2016.30.1.050