한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제50권5호
  • /
  • Pages.624-629
  • /
  • 2017
  • /
  • 0374-8111(pISSN)
  • /
  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
DOI QR코드

DOI QR Code

무지개송어(Onchorhynchus mykiss) 위팽창증후군의 잠재적 원인체의 분자유전학적 동정

Molecular Identification of a Possible Causative Agent of Stomach Distension Syndrome in Rainbow Trout Onchorhynchus mykiss

  • 노형진 (부경대학교 수산생명의학과) ;
  • 김도형 (부경대학교 수산생명의학과)
  • Roh, Heyong Jin (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Do-Hyung (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2017.08.03
  • 심사 : 2017.10.01
  • 발행 : 2017.10.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A rainbow trout Onchorhynchus mykiss farm located in Gangwon province, South Korea, experienced approximately 10% mortality in June 2017. Most diseased fish had a markedly distended, gas-filled stomach, and exhibited abnormal behavior at the water surface. In this study, we attempted to identify the cause of stomach distension syndrome in those rainbow trout. The stomach of most of the affected fish were full of unidentified gases and some exudate, and yeast was isolated from the stomach mucosa. Pure cultures of yeast were identified using a multilocus sequence typing scheme based on 18S rRNA, internal transcribed spacers, large subunit rRNA, and the gene encoding the largest subunit of RNA polymerase II (RPB1). The RPB1 gene sequences were compared with those of related species available in a database. The yeast was identified as Scheffersomyces coipomoensis (Candida coipomoensis) based on sequence analyses. This is the first study to reveal that Sch. coipomoensis is a potential causative agent of stomach distension syndrome in farmed rainbow trout. Our results will be helpful for future related studies, and indicate that farmers and stakeholders should observe this emerging disease closely.

키워드

참고문헌

  1. Awakura T and Kimura T. 1972. Plistophora anguillarum infection found in young eels imported from Formosa A gastric dilatation found in pond-cultured Oncorhynchus masou. Fish Pathol 6, 121-124. http://doi.org/10.3147/jsfp.6.121.
  2. Daniel H, Lachance M and Kurtzman CP. 2014. On the reclassification of species assigned to Candida and other anamorphic ascomycetous yeast genera based on phylogenetic circumscription. Antonie Van Leeuwenhoek 106, 67-84. https://doi.org/10.1007/s10482-014-0170-z.
  3. de Llanos Frutos R, Fernandez-Espinar MT and Querol A. 2004. Identification of species of the genus Candida by analysis of the 5.8 S rRNA gene and the two ribosomal internal transcribed spacers. Antonie Van Leeuwenhoek 85, 175-185. https://doi.org/10.1023/B:ANTO.0000020154.56649.0f.
  4. Diez B, Pedros-Alio C, Marsh TL and Massana R. 2001. Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques. Appl Environ Microbiol 67, 2942-2951. http://doi.org/10.1128/AEM.67.7.2942-2951.2001.
  5. FAO (Food and Agriculture organization of the United Nations). 2015. Cultured aquatic species information programme Oncorhynchus mykiss (Walbaum, 1792). Fisheries and aquaculture department. Retrieved from http://www.fao.org/fishery/culturedspecies/Oncorhynchus_mykiss/en on Aug 02, 2017.
  6. Gall GA and Crandell P. 1992. The rainbow trout. Aquaculture 100, 1-10. https://doi.org/10.1016/0044-8486(92)90333-G.
  7. Egusa S. 1992. Infectious disease of fish. A.A. Balkema Publishers, Rotterdam, Netherlands, 417-419.
  8. Hilton JW and Slinger SJ. 1981. Nutrition and feeding of rainbow trout. Government of Canada, Fisheries and Oceans, Scientific Information and Publications Branch, Ottawa, Canada.
  9. Hitai K and Egusa S. 1975. Candida sake from gastro-tympanites of Amago, Oncorhynchus rhodurus. Bull Jap Soc Sci Fish 41, 993. http://doi.org/10.2331/suisan.41.993.
  10. Kim JW, Lee HN, Jee BY, Woo SH, Kim YJ and Lee MK. 2012. Monitoring of the mortalities in the aquaculture farms of South Korea. J Fish Pathol 25, 271-277. 10.7847/jfp.2012.25.3.271.
  11. Kim SW, Kim JO, Kim WS, Kim DH and Oh MJ. 2014. Vibrio anguillarum infection in rainbow trout Oncorhynchus mykiss during seawater adaption. J Fish Pathol 27, 133-137. 10.7847/jfp.2014.27.2.133.
  12. Kim WS, Kong KH and Oh MJ. 2017. Stomach Distension Syndrome of Seawater Farmed Rainbow Trout (Oncorhynchus mykiss). Korean J Ichthyol 29, 75-79.
  13. KOSTAT (Korean statistics). 2015. The amount of trout production in domestic and its tendency of relevant groups (in Korean). Retrieved from http://www.krtaa.or.kr/info/data.php?ptype=view&idx=5209&page=1&code=data on Aug 02, 2017.
  14. Kumar S, Stecher G and Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33, 1870-1874. http://dx.doi.org/10.1093/molbev/msw054.
  15. Kurtzman CP and Robnett CJ. 2003. Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses. FEMS Yeast Res 3, 417-432. https://doi.org/10.1016/S1567-1356(03)00012-6.
  16. Kurtzman C. 2011. Discussion of teleomorphic and anamorphic ascomycetous yeasts and yeast-like taxa. The Yeasts, a Taxonomic Study 5, 293-307. http://dx.doi.org/10.1016/B978-0-444-52149-1.00013-6.
  17. Lee NS. 2013. The current situation and suggestions of the mariculture of rainbow trout in Korea (in Korean). Oceans and Fisheries 3, 86-112. Retrieved from http://insight.dbpia.co.kr/article/metrics.do?nodeId=NODE02297764# on Aug 02, 2017.
  18. Moon-van der Staay, Seung Yeo, De Wachter R and Vaulot D. 2001. Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity. Nature 409, 607-610. http://dx.doi.org/10.1038/35054541.
  19. Park S, Yu J and Han J. 2012. Studies on the overinflation of the Cardiac Stomach in Sterlet Sturgeon Acipenser ruthenus, fingerlings. J Fish Pathol 25, 59-65. 10.7847/jfp.2012.25.1.059.
  20. Urbina H and Blackwell M. 2013. New combinations, Scheffersomyces amazonensis and S. ergatensis. Mycotaxon 123, 233-234. https://doi.org/10.5248/123.233.
  21. Urbina H and Blackwell M. 2012. Multilocus phylogenetic study of the Scheffersomyces yeast clade and characterization of the N-terminal region of xylose reductase gene. PLoS One 7, e39128. https://doi.org/10.1371/journal.pone.0039128.
  22. van Maris A, Winkler A, Kuyper M, de Laat W, van Dijken J and Pronk J. 2007. Development of efficient xylose fermentation in Saccharomyces cerevisiae: xylose isomerase as a key component. Biofuels, 179-204. https://doi.org/10.1007/10_2007_057.
  23. Mannarelli BM and Kurtzman CP. 1998. Rapid identification of Candida albicans and other human pathogenic yeasts by using short oligonucleotides in a PCR. J Clin Microbiol 36, 1634-1641.