Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibitory Effects of Schisandrae Fructus Ethanol Extract on the Production of Matrix Metalloproteinases in in vitro and in vivo Osteoarthritis Models

In vitro 및 in vivo 퇴행성관절염 모델에서 오미자 에탄올 추출물에 의한 matrix metalloproteinases의 생성 억제

  • Jeong, Jin-Woo (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine) ;
  • Lee, Hye Hyeon (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine) ;
  • Kim, Hong Jae (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine) ;
  • Lee, Ki Won (Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center) ;
  • Kim, Ki Young (Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center) ;
  • Kim, Sung Goo (Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center) ;
  • Hong, Su-Hyun (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine) ;
  • Kim, Bum Hoi (Department of Anatomy, Dongeui University College of Korean Medicine) ;
  • Park, Cheol (Department of Molecular Biology, College of Natural Sciences, Dongeui University) ;
  • Choi, Yung Hyun (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine)
  • 정진우 (동의대학교 근.골격계 질환제어 융합연구실 및 한의과대학 생화학교실) ;
  • 이혜현 (동의대학교 근.골격계 질환제어 융합연구실 및 한의과대학 생화학교실) ;
  • 김홍재 (동의대학교 근.골격계 질환제어 융합연구실 및 한의과대학 생화학교실) ;
  • 이기원 ((주)바이오포터코리아) ;
  • 김기영 ((주)바이오포터코리아) ;
  • 김성구 ((주)바이오포터코리아) ;
  • 홍수현 (동의대학교 근.골격계 질환제어 융합연구실 및 한의과대학 생화학교실) ;
  • 김범회 (동의대학교 한의과대학 해부학교실) ;
  • 박철 (동의대학교 자연과학대학 분자생물학과) ;
  • 최영현 (동의대학교 근.골격계 질환제어 융합연구실 및 한의과대학 생화학교실)
  • Received : 2017.09.06
  • Accepted : 2017.09.11
  • Published : 2017.10.30
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Abstract

Schisandrae Fructus (SF), the fruit of Schisandra chinensis (Turcz.) Baill., is widely used in traditional medicine for the treatment of a number of chronic diseases. SF extracts have been recently reported to attenuate the inflammatory responses in SW1353 human chondrocyte cells in in vitro and monosodium iodoacetate (MIA)-induced cartilage degradation in in vivo osteoarthritis (OA) models. However, their protective and therapeutic potentials against OA in primary culture chondrocytes and animal models remain unclear. Therefore, we investigated the effects of the ethanol extract of SF on the activity of matrix metalloproteinases (MMPs), biomarkers for diagnosis of OA, on interleukin $(IL)-1{\beta}-induced$ primary cultured rat cartilage chondrocytes and MIA-induced osteoarthritis in a rat model. Our data indicated that SF treatment significantly reduced the mRNA expression and enzyme activity of MMP-1, -3 and -13 in $IL-1{\beta}-induced$ primary cultured rat cartilage chondrocytes. The chondro-protective effects of SF were then analyzed in a rat OA model using a single intra-articular injection of MIA in the right knee joint. According to our results, the elevated levels of MMP-1 and -3 were markedly ameliorated by SF administration. Collectively, these findings indicate that SF could be a candidate for the treatment of OA.

노화에 따른 퇴행성관절염은 삶의 질을 저하시키는 가장 큰 병리학적 현상 중의 하나이다. 오미자 열매(Schisandrae Fructus)는 오랫동안 전통의학에서 여러 가지 만성질환 치료를 위해 널리 사용되어 왔다. 오미자 추출물이 SW1353 인간 연골세포에서 $IL-1{\beta}$에 의해 유발된 염증 반응을 감소시키는 것으로 최근 보고된 바 있으나, primary culture된 연골세포 및 동물 모델에서의 퇴행성관절염에 대한 보호 및 치료 잠재력은 여전히 명확하지 않다. 따라서 본 연구에서는 $IL-1{\beta}$에 의해 유도된 primary culture된 쥐의 연골세포와 MIA에 의해 유도된 골관절염에 대한 matrix metalloproteinases (MMPs)의 활성에 미치는 오미자 에탄올 추출물의 영향을 조사하였다. 오미자 추출물 처리는 $IL-1{\beta}$로 유도된 연골세포에서 MMP-1, -3 및 -13의 mRNA 발현 및 효소 활성을 유의하게 감소시켰다. 또한 오미자 추출물은 MIA에 의해 증가된 MMP-1 및 -3의 발현을 유의적으로 억제시켰다. 따라서 오미자 추출물은 퇴행성관절염 예방과 치료를 위한 기능성 소재로서의 잠재적 가능성이 있음을 알 수 있었다.

Keywords

References

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