Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Study on the Genetic Characteristics of Waterlogging Tolerant Pepper (Capsicum annuum L.) for Breeding Tolerant Varieties against Flooding Stress

내습성 고추 품종 육성을 위한 선발계통의 유전적 특성 구명

  • Yang, Eun Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Chae, Soo-Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Hong, Jong-Pil (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Hye-Eun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Eun Joon (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Moon, Ji-hye (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Tae-Sung (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Roh, Mi-Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Ok Rye (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Sang Gyu (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Dae Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Sun Yi (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Myeong Cheoul (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 양은영 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 채수영 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 홍종필 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 이혜은 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 박은준 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 문지혜 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 박태성 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 노미영 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 김옥례 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 김상규 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 김대영 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 이선이 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 조명철 (농촌진흥청 국립원예특작과학원 채소과)
  • Received : 2017.09.01
  • Accepted : 2017.10.26
  • Published : 2017.10.30
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Abstract

This study was conducted to select pepper lines that were tolerant to excessive water injury among the pepper germplasm and investigate the genetic characteristics of those lines to contribute to the breeding of pepper cultivars with stable productivity in abnormal weather. Each of the tolerant and susceptible lines went through immersion treatment, and differentially expressed genes between them were analyzed. The tolerant line showed increased expression of the CA02g26670 gene, which is involved in the CONSTANS protein pathway and regulation of flowering by day length, but it exhibited decreased expressions of CA01g21450, CA01g22480, CA01g34470, CA02g00370 and CA02g00380. The susceptible line showed increased gene expressions of CA02g09720, CA02g21290, CA03g16520, CA07g 02110, and CA12g17910, which are involved in the inhibition of proteolytic enzyme activity, DNA binding, inhibition of cell wall-degrading enzyme, and inhibition of nodulin, respectively. Meanwhile the expressions of CA02g02820, CA03g21390, CA06g17700 and CA07g18230 decreased in the susceptible line, in relation to calcium-ion binding, high temperature, synthesis of phosphocholine and cold stress, respectively. The expressions of genes related to apoptosis and peroxidase increased, while that of CA02g16990, which functions as a nucleoside transporter, decreased in both the tolerant and susceptible lines. Based on the different gene expressions between the tolerant and susceptible lines, further studies are needed on breeding abiotic stress-tolerant lines.

내습성 및 감수성 선발계통 각 1점씩을 대상으로 침수처리 후 GO 분석을 통해 추출된 유전자들을 기능별로 분류해보면 생물학적 과정(biological process)에 관여하는 유전자 발현이 가장 크게 영향을 받았으며, 분자 기능(molecular function)과 세포 요소(cellular component) 관련 유전자를 포함하여 모든 기능의 유전자 발현 변화가 감수성 계통보다 내습성 계통에서 크게 일어났다. 침수처리 후 발현 양상이 유의하게 차이나는 유전자의 보다 자세한 기능을 측정한 결과 내습성 계통에서 발현량이 증가한 유전자는 CA02g26670은 CONSTANS protein과 관련있는 유전자로 일장조건에 따른 개화조절에 관여하는 유전자이며, 발현량이 감소한 유전자는 CA01g21450, CA01g22480, CA01g34470, CA02g00370, CA02g00380이었다. 감수성 계통에서 침수처리 후 발현량이 증가한 유전자는 CA02g09720, CA02g21290, CA03g16520, CA07g02110, CA12g17910이었는데 각각 단백질 분해효소 활성 저해, DNA binding, 세포벽 분해효소 억제, nodulin 관련 유전자 등으로 밝혀진 유전자들이었다. 감수성 계통에서 발현량이 감소한 유전자는 CA02g02820, CA03g21390, CA06g17700, CA07g18230로 각각 칼슘이온결합, 고온환경 발현기작, 레시틴 생합성 경로의 수용성중간대사체인 phosphocholine 합성 및 저온스트레스 관련 등의 기능을 한다. 내습성 계통과 감수성 계통에서 동시에 발현이 증가한 유전자는 고온 등 환경스트레스로 인한 apoptosis와 관련된 유전자와 peroxidase와 관련있는 유전자로 확인되었고, 발현이 동시에 감소한 유전자는 nucleoside transporter인 CA02g16990로 확인되었으며, 나머지 선발 유전자는 유전자 발현이 확인되지 않았다. 내습성 및 감수성 계통간 습해 조건에서 발현에 차이를 보이는 유전자 구명을 기반으로 향후 불량환경에 대해 저항성을 보이는 계통 육성 및 관련 생리반응에 대한 다양한 연구가 필요하다.

Keywords

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