생명과학회지 (Journal of Life Science)

  • 제27권10호
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  • Pages.1097-1103
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  • 2017
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Osmotic pump를 이용한 암컷 뱀장어(Anguilla japonica)의 성성숙 유도

Induction of Sexual Maturation in Female Eels (Anguilla japonica) Using an Osmotic Pump

  • 김정현 (국립수산과학원 전략양식부 양식관리과) ;
  • 김대근 (국립수산과학원 전략양식부 양식관리과) ;
  • 김효원 (국립수산과학원 전략양식부 양식관리과) ;
  • 이배익 (국립수산과학원 전략양식부 양식관리과) ;
  • 김신권 (국립수산과학원 전략양식부 양식관리과) ;
  • 전제천 (국립수산과학원 전략양식부 양식관리과) ;
  • 명정인 (국립수산과학원 전략양식부 양식관리과) ;
  • 김대중 (국립수산과학원 전략양식부 양식관리과)
  • Kim, Jung-Hyun (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Kim, Dae-Geun (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Kim, Hyo-Won (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Lee, Bae-Ik (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Kim, Shin-Kwon (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Jun, Je-cheon (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Myeong, Jeong-In (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Kim, Dae-Jung (Aquaculture Research Division, National Institute of Fisheries Science)
  • 투고 : 2017.07.24
  • 심사 : 2017.10.20
  • 발행 : 2017.10.30
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초록

뱀장어(Anguilla japonica)의 인공종묘생산은 연어뇌하수체추출물(SPE, salmon pituitary extract)을 암컷 뱀장어에게 지속적인 투여를 하고 인위적으로 성성숙을 유도로 얻은 난과 정자를 인공수정을 하고 부화 시켜 생산한다. 하지만 SPE를 반복적으로 복강에 주사하는 방법은 암컷 뱀장어에게 많은 스트레스를 주며, 결국 스트레스로 인하여 완전한 성성숙을 하지 못하고 폐사에 이르게 되거나 배란한 난의 질이 좋지 않아 부화율과 자어의 생존율이 떨어지는 문제점을 갖고 있다. 본 연구에서는 뱀장어의 성성숙 유도에 유효하다고 알려진 호르몬이 주입된 osmotic pump를 복강에 삽입 후 암컷 뱀장어의 성성숙 유도를 하였다. 본 연구결과 SPE를 투여한 실험어의 GSI가 hCG, GnRHa, MT를 각각 또는 함께 투여한 실험구 보다 유의하게 증가하였으며 조직학적 분석결과에서도 난소의 난모세포가 핵이동기 단계로 발달되었음을 관찰할 수 있었다. 본 결과를 통해 osmotic pump를 이용한 암컷 뱀장어 인위적 성성숙 유도 방법으로 이용 가능할 것으로 생각된다.

For the artificial induction of the sexual maturation of Anguilla japonica, salmon pituitary extract (SPE) is continuously injected into females, and the eggs obtained from artificial sexual maturation are artificially fertilized with sperms and hatched. However, repeated injection of SPE in the abdominal cavity causes tremendous stress in females, which may prevent their complete sexual maturation and reduce the immune system function, ultimately resulting in death. In addition, the poor quality of the ovulated eggs can reduce the hatching and survival rate of larvae. In the present study, sexual maturation of females was induced by inserting an osmotic (OS) pump containing hormone analogs known to effectively induce sexual maturation into the abdominal cavity of female eels, and the effect of the OS pump on the induced sexual maturation was investigated. Our study results showed that the gonadosomatic index (GSI) was significantly higher in the fish subjected to SPE injection than those subjected to human chorionic gonadotropin (hCG), gonadotropin-releasing hormone analog (GnRHa), and methyl testosterone (MT) injections, either separately or in combination. In addition, a histological analysis showed that the oocytes in the SPE OS pump groups were more mature (entered the nuclear shift stage) than those in the other groups. These results suggest that an osmotic pump containing hormone analogs can be used to induce sexual maturation in female A. japonica artificially.

키워드

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