Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Induction of Sexual Maturation in Female Eels (Anguilla japonica) Using an Osmotic Pump

Osmotic pump를 이용한 암컷 뱀장어(Anguilla japonica)의 성성숙 유도

  • Kim, Jung-Hyun (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Kim, Dae-Geun (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Kim, Hyo-Won (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Lee, Bae-Ik (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Kim, Shin-Kwon (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Jun, Je-cheon (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Myeong, Jeong-In (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Kim, Dae-Jung (Aquaculture Research Division, National Institute of Fisheries Science)
  • 김정현 (국립수산과학원 전략양식부 양식관리과) ;
  • 김대근 (국립수산과학원 전략양식부 양식관리과) ;
  • 김효원 (국립수산과학원 전략양식부 양식관리과) ;
  • 이배익 (국립수산과학원 전략양식부 양식관리과) ;
  • 김신권 (국립수산과학원 전략양식부 양식관리과) ;
  • 전제천 (국립수산과학원 전략양식부 양식관리과) ;
  • 명정인 (국립수산과학원 전략양식부 양식관리과) ;
  • 김대중 (국립수산과학원 전략양식부 양식관리과)
  • Received : 2017.07.24
  • Accepted : 2017.10.20
  • Published : 2017.10.30
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Abstract

For the artificial induction of the sexual maturation of Anguilla japonica, salmon pituitary extract (SPE) is continuously injected into females, and the eggs obtained from artificial sexual maturation are artificially fertilized with sperms and hatched. However, repeated injection of SPE in the abdominal cavity causes tremendous stress in females, which may prevent their complete sexual maturation and reduce the immune system function, ultimately resulting in death. In addition, the poor quality of the ovulated eggs can reduce the hatching and survival rate of larvae. In the present study, sexual maturation of females was induced by inserting an osmotic (OS) pump containing hormone analogs known to effectively induce sexual maturation into the abdominal cavity of female eels, and the effect of the OS pump on the induced sexual maturation was investigated. Our study results showed that the gonadosomatic index (GSI) was significantly higher in the fish subjected to SPE injection than those subjected to human chorionic gonadotropin (hCG), gonadotropin-releasing hormone analog (GnRHa), and methyl testosterone (MT) injections, either separately or in combination. In addition, a histological analysis showed that the oocytes in the SPE OS pump groups were more mature (entered the nuclear shift stage) than those in the other groups. These results suggest that an osmotic pump containing hormone analogs can be used to induce sexual maturation in female A. japonica artificially.

뱀장어(Anguilla japonica)의 인공종묘생산은 연어뇌하수체추출물(SPE, salmon pituitary extract)을 암컷 뱀장어에게 지속적인 투여를 하고 인위적으로 성성숙을 유도로 얻은 난과 정자를 인공수정을 하고 부화 시켜 생산한다. 하지만 SPE를 반복적으로 복강에 주사하는 방법은 암컷 뱀장어에게 많은 스트레스를 주며, 결국 스트레스로 인하여 완전한 성성숙을 하지 못하고 폐사에 이르게 되거나 배란한 난의 질이 좋지 않아 부화율과 자어의 생존율이 떨어지는 문제점을 갖고 있다. 본 연구에서는 뱀장어의 성성숙 유도에 유효하다고 알려진 호르몬이 주입된 osmotic pump를 복강에 삽입 후 암컷 뱀장어의 성성숙 유도를 하였다. 본 연구결과 SPE를 투여한 실험어의 GSI가 hCG, GnRHa, MT를 각각 또는 함께 투여한 실험구 보다 유의하게 증가하였으며 조직학적 분석결과에서도 난소의 난모세포가 핵이동기 단계로 발달되었음을 관찰할 수 있었다. 본 결과를 통해 osmotic pump를 이용한 암컷 뱀장어 인위적 성성숙 유도 방법으로 이용 가능할 것으로 생각된다.

Keywords

References

  1. Aida, K. 1983. Effect of LH-releasing hormone on gonadal develop mentin salmonid fish, the Ayu. Bull. Jpn. Soc. Sci. Fish. 49, 711-718. https://doi.org/10.2331/suisan.49.711
  2. Bae, J. Y., Kim, D. J., Lee, J. U., Son, S. G. and Lee, J. K. 2007. Correlationship between artificial maturation season and reproduction coefficient in the cultured eel Anguilla japonica. J. Aquacult. 20, 219-225.
  3. Crim, L. W., Sutterlin, A. M., Evans, D. M. and Weil, C. 1983. Accelerated ovulation by pelleted LHRH analogue treatment of spring-spawning rainbow trout (Salmo gairdneri) held at low temperature. Aquaculture 35, 299-307. https://doi.org/10.1016/0044-8486(83)90102-3
  4. Dufour, S., Bassompierre, M., Montero, M., Belle, N. L., Baloche, S. and Fontaine, Y. A. 1991. Stimulation of pituitary gonadotropic function in female silver eel treated by a gonadoliberin agonist and dopamine antagonists. In: Proc. The Fourth International Symposium on the Reproductive Physiology of Fish, pp. 54-56. Edited by A.P. Scott, J.P. Sumpter, D.E. Kime and M.S. Rolfe. Fish Symp 91, Sheffield, UK.
  5. Dufour, S., Burzawa-Gerard, E., Le Belle, N., Sbaihi, M. and Vidal, B. 2003. Reproductive endocrinology of the European eel, Anguilla anguilla. In Eel biology (pp. 373-383).
  6. Gibson, M. J., Kasowski, H. and Dobrjansky, A. 1994. Continuous gonadotropin-releasing hormone infusion stimulates dramatic gonadal development in hypogonadal female mice. Biol. Reprod. 50, 680-685. https://doi.org/10.1095/biolreprod50.3.680
  7. Girling, J. E., Bennett, E. J. and Cockrem, J. F. 2002. Administration of pregnant mare serum gonadotropin to Japanese quail (Coturnix coturnix japonica): dose response over seven days and comparison of delivery by daily injection or osmotic pump. N. Z. Vet. J. 50, 115-121.
  8. Ijiri, S., Kayaba, T., Takeda, N., Tachiki, H., Adachi, S. and Yamauchi, K. 1998. Pretreatment reproductive stage and oocyte development induced by salmon pituitary homogenate in the Japanese eel Anguilla japonica. Fish. Sci. 64, 531-537. https://doi.org/10.2331/fishsci.64.531
  9. Kagawa, H., Tanaka, H., Ohta, H., Unuma, T. and Nomura, K. 2005. The first success of glass eel production in the world: basic biology on fish reproduction advances new applied technology in aquaculture. Fish Physiol. Biochem. 31, 193-199. https://doi.org/10.1007/s10695-006-0024-3
  10. Kagawa, H., Kasuga, Y., Adachi, J., Nishi, A., Hashimoto, H., Imaizumi, H. and Kaji, S. 2009. Effects of continuous administration of human chorionic gonadotropin, salmon pituitary extract, and gonadotropin-releasing hormone using osmotic pumps on induction of sexual maturation in male Japanese eel, Anguilla japonica. Aquaculture 296, 117-122. https://doi.org/10.1016/j.aquaculture.2009.07.023
  11. Kagawa, H., Fujie, N., Imaizumi, H., Masuda, Y., Oda, K., Adachi, J. and Kaji, S. 2013. Using osmotic pumps to deliver hormones to induce sexual maturation of female Japanese eels, Anguilla japonica. Aquaculture 388, 30-34.
  12. Kim, D. J., Kang, E. J., Bae, J. Y., Park, M. W. and Kim, E. O. 2007. Development of the eggs and pre-leptocephalus Larvae by natural spawning of artificially-matured Japanese eel, Anguilla japonica. J. Aquacult. 20, 160-167.
  13. Kim, D. J., Lee, B. I., Kim, K. K., Kim, E. O., Son, M. H. and Seong, K. B. 2013. Effects of estradiol-$17{\beta}$ on the feminization of Japanase eel, Anguilla japonica. J. Life Sci. 23, 998-1003. https://doi.org/10.5352/JLS.2013.23.8.998
  14. Kim, D. J., Kim, Y. C., Choi, Y. K., Son, M. H., Lee, J. U., Park, M. S. and Heo, Y. S. 2009. Effects of rearing condition during the winter period on artificial maturation and reproduction of cultured female eel, Anguilla japonica. Dev. Reprod. 13, 35-41.
  15. Kim, D. J. and Aida, K. 2000. Dopaminergic regulation of Gonadotropin-II secretion in testosterone-treated precocious male and immature rainbow trout oncorhynchus mykiss. Kor. J. Biol. Sci. 4, 287-292. https://doi.org/10.1080/12265071.2000.9647558
  16. Moussavi, M., Nelson, E. R. and Habibi, H. R. 2009. Seasonal regulation of vitellogenin by growth hormone in the goldfish liver. Gen. Comp. Endocrinol. 161, 79-82. https://doi.org/10.1016/j.ygcen.2008.12.009
  17. Mylonas, C. C. and Zohar, Y. 2000. Use of GnRHa-delivery systems for the control of reproduction in fish. Rev. Fish Biol. Fish. 10, 463-491. https://doi.org/10.1023/A:1012279814708
  18. Nagae, M., Todo, T., Gen, K., Kato, Y., Young, G., Adachi, S. and Yamauchi, K. 1996. Molecular cloning of the cDNAs encoding pituitary glycoprotein hormone ${\alpha}$-and gonadotropin $II{\beta}$-subunits of the Japanese eel, Anguilla japonica, and increase in their mRNAs during ovarian development induced by injection of chum salmon pituitary homogenate. J. Mol. Endocrinol. 16, 171-181. https://doi.org/10.1677/jme.0.0160171
  19. Ohta, H., Kagawa, H., Tanaka, H., Okuzawa, K. and Hirose, K. 1996. Changes in fertilization and hatching rates with time after ovulation induced by 17, $20{\beta}$-dihydroxy-4-pregnen-3-one in the Japanese eel, Anguilla japonica. Aquaculture 139, 291-301. https://doi.org/10.1016/0044-8486(95)01167-6
  20. Ohta, H., Kagawa, H., Tanaka, H., Okuzawa, K. and Hirose, K. 1996. Milt production in the Japanese eel Anguilla japonica induced by repeated injections of human chorionic gonadotropin. Fish. Sci. 62, 44-49. https://doi.org/10.2331/fishsci.62.44
  21. Patton, P. E., Stouffer, R. L. and Zelinski-Wooten, M. B. 1990. The effect of intraluteal infusion of deglycosylated human chorionic gonadotropin on the corpus luteum in rhesus monkeys. J. Clin. Endocrinol. Metab. 70, 1213-1218. https://doi.org/10.1210/jcem-70-4-1213
  22. Rocha, A., Gomez, A., Galay-Burgos, M., Zanuy, S., Sweeney, G. E. and Carrillo, M. 2007. Molecular characterization and seasonal changes in gonadal expression of a thyrotropin receptor in the European sea bass. Gen. Comp. Endocrinol. 152, 89-101. https://doi.org/10.1016/j.ygcen.2007.03.001
  23. Sato, N., Kawazoel, I., Suzuki, Y. and Aida, K. 2003. Induction of vitellogenesis. In Eel Biology (pp. 387-399).
  24. Sato, N., Kawazoe, I., Shiina, Y., Furukawa, K., Suzuki, Y. and Aida, K. 1995. A novel method of hormone administration for inducing gonadal maturation in fish. Aquaculture 135, 51-58. https://doi.org/10.1016/0044-8486(95)01007-6
  25. Tamaru, C. S., Kelley, C. D., Lee, C. S., Aida, K. and Hanyu, I. 1989. Effects of chronic LHRH-a+ 17-methyltestosterone or LHRH-a+ testosterone therapy on oocyte growth in the striped mullet (Mugil cephalus). Gen. Comp. Endocrinol. 76, 114-127. https://doi.org/10.1016/0016-6480(89)90038-5
  26. Unuma, T., Kondo, S., Tanaka, H., Kagawa, H., Nomura, K. and Ohta, H. 2004. Determination of the rates of fertilization, hatching and larval survival in the Japanese eel, Anguilla japonica, using tissue culture microplates. Aquaculture 241, 345-356. https://doi.org/10.1016/j.aquaculture.2004.08.005
  27. Yamamoto, K., Oomori, M. and Yamauchi, K. 1974. Oogenesis of the Japanese eel. Nippon Suisan Gakkaishi. 40, 9-15. https://doi.org/10.2331/suisan.40.9
  28. Zohar, Y. and Mylonas, C. C. 2001. Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture 197, 99-136. https://doi.org/10.1016/S0044-8486(01)00584-1