Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Effects of Oleo Gum Resin of Ferula assa-foetida L. on Senescence in Human Dermal Fibroblasts - Asafoetida reverses senescence in fibroblasts -

  • Received : 2017.07.30
  • Accepted : 2017.09.07
  • Published : 2017.09.30
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Abstract

Objectives: Based on data from Chinese and Indian traditional herbal medicines, gum resin of Ferula assa-foetida (sometimes referred to asafetida or asafoetida) has several therapeutic applications. The authors of various studies have claimed that asafetida has cytotoxic, antiulcer, anti-neoplasm, anti-cancer, and anti-oxidative effects. In present study, the anti-aging effect of asafetida on senescent human dermal fibroblasts was evaluated. Methods: Senescence was induced in in vitro cultured human dermal fibroblasts (HDFs) through exposure to $H_2O_2$, and the incidence of senescence was recognized by using cytochemical staining for the activity of ${\beta}$-galactosidase. Then, treatment with oleo gum resin of asafetida was started to evaluate its rejuvenating effect. The survival rate of fibroblasts was evaluated by using methyl tetrazolium bromide (MTT) assays. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blot assays were performed to evaluate the expressions of apoptotic and anti-apoptotic markers. Results: Our experiments show that asafetida in concentrations ranging from $5{\times}10^{-8}$ to $10^{-7}g/mL$ has revitalizing effects on senescent fibroblasts and significantly reduces the ${\beta}$-galactosidase activity in these cells (P < 0.05). Likewise, treatment at these concentrations increases the proliferation rate of normal fibroblasts (P < 0.05). However, at concentrations higher than $5{\times}10^{-7}g/mL$, asafetida is toxic for cells and induces cell death. Conclusion: The results of this study indicate that asafetida at low concentrations has a rejuvenating effect on senescent fibroblasts whereas at higher concentrations, it has the opposite effect of facilitating cellular apoptosis and death.

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References

  1. Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2013;4:177.
  2. Pan SY, Litscher G, Gao SH, Zhou SF, Yu ZL, Chen HQ, et al. Historical perspective of traditional indigenous medical practices: the current renaissance and conservation of herbal resources. Evid Based Complement Alternat Med. 2014;2014:DOI: 10.1155/2014/525340.
  3. Di Lorenzo C, Ceschi A, Kupferschmidt H, Lude S, De Souza Nascimento E, Dos Santos A, et al. Adverse effects of plant food supplements and botanical preparations: a systematic review with critical evaluation of causality. Br J Clin Pharmacol. 2015;79(4):578-92. https://doi.org/10.1111/bcp.12519
  4. Mahendra P, Bisht S. Ferula asafoetida: traditional uses and pharmacological activity. Pharmacogn Rev. 2012;6(12):141-6. https://doi.org/10.4103/0973-7847.99948
  5. Kareparamban JA, Nikam PH, Jadhav AP, Kadam VJ. A validated high-performance liquid chromatograhy method for estimation of ferulic acid in asafoetida and polyherbal preparation. Indian J Pharm Sci. 2013;75(4):493-5. https://doi.org/10.4103/0250-474X.119826
  6. Qiao Y, He H, Zhang Z, Liao Z, Yin D, Liu D, et al. Longterm sodium ferulate supplementation scavenges oxygen radicals and reverses liver damage induced by iron overloading. Molecules. 2016;21(9):E1219. https://doi.org/10.3390/molecules21091219
  7. Hahn HJ, Kim KB, Bae S, Choi BG, An S, Ahn KJ, et al. Pretreatment of ferulic acid protects human dermal fibroblasts against ultraviolet a irradiation. Ann Dermatol. 2016;28(6):740-8. https://doi.org/10.5021/ad.2016.28.6.740
  8. Ouimet MA, Faig JJ, Yu W, Uhrich KE. Ferulic acidbased polymers with glycol functionality as a versatile platform for topical applications. Biomacromolecules. 2015;16(9):2911-9. https://doi.org/10.1021/acs.biomac.5b00824
  9. Graf E. Antioxidant potential of ferulic acid. Free Radic Biol Med. 1992;13(4):435-48. https://doi.org/10.1016/0891-5849(92)90184-I
  10. Trombino S, Serini S, Di Nicuolo F, Celleno L, Ando S, Picci N, et al. Antioxidant effect of ferulic acid in isolated membranes and intact cells: synergistic interactions with alpha-tocopherol, beta-carotene, and ascorbic acid. J Agric Food Chem. 2004;52(8):2411-20. https://doi.org/10.1021/jf0303924
  11. Kikuzaki H, Hisamoto M, Hirose K, Akiyama K, Taniguchi H. Antioxidant properties of ferulic acid and its related compounds. J Agric Food Chem. 2002;50(7):2161-8. https://doi.org/10.1021/jf011348w
  12. Kaul A, Khanduja KL. Polyphenols inhibit promotional phase of tumorigenesis: relevance of superoxide radicals. Nutr Cancer.1998;32(2):81-5. https://doi.org/10.1080/01635589809514723
  13. Hynes MJ, O'Coinceanainn M. The kinetics and mechanisms of reactions of iron(III) with caffeic acid, chlorogenic acid, sinapic acid, ferulic acid and naringin. J Inorg Biochem. 2004;98(8):1457-64. https://doi.org/10.1016/j.jinorgbio.2004.05.009
  14. Wenk GL, McGann-Gramling K, Hauss-Wegrzyniak B, Ronchetti D, Maucci R, Rosi S, et al. Attenuation of chronic neuroinflammation by a nitric oxide-releasing derivative of the antioxidant ferulic acid. J Neurochem. 2004;89(2):484-93. https://doi.org/10.1111/j.1471-4159.2004.02359.x
  15. Ogiwara T, Satoh K, Kadoma Y, Murakami Y, Unten S, Atsumi T, et al. Radical scavenging activity and cytotoxicity of ferulic acid. Anticancer Res. 2002;22(5):2711-7.
  16. Pannala AS, Razaq R, Halliwell B, Singh S, Rice-Evans CA. Inhibition of peroxynitrite dependent tyrosine nitration by hydroxycinnamates: nitration or electron donation?. Free Radic Biol Med. 1998;24(4):594-606. https://doi.org/10.1016/S0891-5849(97)00321-3
  17. Dinis TC, Santosa CL, Almeida LM. The apoprotein is the preferential target for peroxynitrite-induced LDL damage protection by dietary phenolic acids. Free Radic Res. 2002;36(5):531-43. https://doi.org/10.1080/10715760290025915
  18. Yamada J, Tomita Y. Antimutagenic activity of caffeic acid and related compounds. Biosci Biotechnol Biochem. 1996;60(2):328-9. https://doi.org/10.1271/bbb.60.328
  19. Ferguson LR, Lim IF, Pearson AE, Ralph J, Harris PJ. Bacterial antimutagenesis by hydroxycinnamic acids from plant cell walls. Mutat Res. 2003;542(1-2):49-58. https://doi.org/10.1016/j.mrgentox.2003.08.005
  20. Lesca P. Protective effects of ellagic acid and other plant phenols on benzo[a]pyrene-induced neoplasia in mice. Carcinogenesis. 1983;4(12):1651-3. https://doi.org/10.1093/carcin/4.12.1651
  21. Burdette JE, Chen SN, Lu ZZ, Xu H, White BE, Fabricant DS, et al. Black cohosh (Cimicifuga racemosa L.) protects against menadione-induced DNA damage through scavenging of reactive oxygen species: bioassay-directed isolation and characterization of active principles. J Agric Food Chem. 2002;50(24):7022-8. https://doi.org/10.1021/jf020725h
  22. Kawabata K, Yamamoto T, Hara A, Shimizu M, Yamada Y, Matsunaga K, et al. Modifying effects of ferulic acid on azoxymethane-induced colon carcinogenesis in F344 rats. Cancer Lett. 2000;157(1):15-21. https://doi.org/10.1016/S0304-3835(00)00461-4
  23. Wargovich MJ, Jimenez A, McKee K, Steele VE, Velasco M, Woods J, et al. Efficacy of potential chemopreventive agents on rat colon aberrant crypt formation and progression. Carcinogenesis. 2000;21(6):1149-55. https://doi.org/10.1093/carcin/21.6.1149
  24. Saija A, Tomaino A, Trombetta D, De Pasquale A, Uccella N, Barbuzzi T, et al. In vitro and in vivo evaluation of caffeic and ferulic acids as topical photoprotective agents. Int J Pharm. 2000;199(1):39-47. https://doi.org/10.1016/S0378-5173(00)00358-6
  25. Huang MT, Smart RC, Wong CQ, Conney AH. Inhibitory effect of curcumin, chlorogenic acid, caffeic acid, and ferulic acid on tumor promotion in mouse skin by 12-O-tetradecanoylphorbol-13-acetate. Cancer Res. 1988;48(21):5941-6.
  26. Gardin C, Piattelli A, Zavan B. Graphene in regenerative medicine: focus on stem cells and neuronal differ-entiation. Trends Biotechnol. 2016;34(6):435-7. https://doi.org/10.1016/j.tibtech.2016.01.006
  27. Mercier B, Prost J, Prost M. The essential oil of turpentine and its major volatile fraction (alpha- and betapinenes): a review. Int J Occup Med Environ Health. 2009;22(4):331-42. https://doi.org/10.2478/v10001-009-0032-5
  28. Shan Y, Wei Z, Tao L, Wang S, Zhang F, Shen C, et al. Prophylaxis of diallyl disulfide on skin carcinogenic model via p21-dependent Nrf2 stabilization. Sci Rep. 2016;6:35676. https://doi.org/10.1038/srep35676
  29. Wang HC, Yang JH, Hsieh SC, Sheen LY. Allyl sulfides inhibit cell growth of skin cancer cells through induction of DNA damage mediated G2/M arrest and apoptosis. J Agric Food Chem. 2010;58(11):7096-103. https://doi.org/10.1021/jf100613x
  30. Palombo R, Savini I, Avigliano L, Madonna S, Cavani A, Albanesi C, et al. Luteolin-7-glucoside inhibits IL-22/STAT3 pathway, reducing proliferation, acanthosis, and inflammation in keratinocytes and in mouse psoriatic model. Cell Death Dis. 2016;7(8):e2344. https://doi.org/10.1038/cddis.2016.201
  31. Kleiner HE, Vulimiri SV, Starost MF, Reed MJ, DiGiovanni J. Oral administration of the citrus coumarin, isopimpinellin, blocks DNA adduct formation and skin tumor initiation by 7,12-dimethylbenz[a]anthracene in SENCAR mice. Carcinogenesis. 2002;23(10):1667-75. https://doi.org/10.1093/carcin/23.10.1667
  32. Ueki JI, Sakagami H, Wakabayashi H. Anti-UV activity of newly-synthesized water-soluble azulenes. In vivo. 2013;27(1):119-26.
  33. Hardwick JM, Soane L. Multiple functions of BCL-2 family proteins. Cold Spring Harb Perspect Biol. 2013;5(2):DOI: 10.1101/cshperspect.a008722.
  34. Chen L, Willis SN, Wei A, Smith BJ, Fletcher JI, Hinds MG, et al. Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function. Mol Cell. 2005;17(3):393-403. https://doi.org/10.1016/j.molcel.2004.12.030
  35. Li T, Kon N, Jiang L, Tan M, Ludwig T, Zhao Y, et al. Tumor suppression in the absence of p53-mediated cell cycle arrest, apoptosis, and senescence. Cell. 2012;149(6):1269-83. https://doi.org/10.1016/j.cell.2012.04.026
  36. Salvesen GS. Caspases: opening the boxes and interpreting the arrows. Cell Death Differ. 2002;9(1):3-5. https://doi.org/10.1038/sj.cdd.4400963
  37. Brogliato AR, Moor AN, Kesl SL, Guilherme RF, Georgii JL, Peters-Golden M, et al. Critical role of 5-lipoxygenase and heme oxygenase-1 in wound healing. J Invest Dermatol. 2014;134(5):1436-45. https://doi.org/10.1038/jid.2013.493
  38. Homayouni Moghadam F, Dehghan M, Zarepur E, Dehlavi R, Ghaseminia F, Ehsani S, et al. Oleo gum resin of Ferula assa-foetida L. ameliorates peripheral neuropathy in mice. J Ethnopharmacol. 2014;154(1):183-9. https://doi.org/10.1016/j.jep.2014.03.069
  39. Chen JH, Stoeber K, Kingsbury S, Ozanne SE, Williams GH, Hales CN. Loss of proliferative capacity and induction of senescence in oxidatively stressed human fibroblasts. J Biol Chem. 2004;279(47):49439-46. https://doi.org/10.1074/jbc.M409153200
  40. Xing Y, Li N, Zhou D, Chen G, Jiao K, Wang W, et al. Sesquiterpene coumarins from ferula sinkiangensis act as neuroinflammation inhibitors. Planta Med. 2017;83(1-02):135-42. https://doi.org/10.1055/s-0042-109271
  41. Asghari J, Atabaki V, Baher E, Mazaheritehrani M. Identification of sesquiterpene coumarins of oleo-gum resin of Ferula assa-foetida L. from the Yasuj region. Nat Prod Res. 2016;30(3):350-3. https://doi.org/10.1080/14786419.2015.1050669
  42. Znati M, Filali I, Jabrane A, Casanova J, Bouajila J, Ben Jannet H. Chemical composition and in vitro evalu-ation of antimicrobial, antioxidant and antigerminative properties of the seed oil from the tunisian endemic ferula tunetana pomel ex batt. Chem Biodivers. 2017;14(1):DOI: 10.1002/cbdv.201600116.
  43. Munoz-Espin D, Serrano M. Cellular senescence: from physiology to pathology. Nat Rev Mol Cell Biol. 2014;15(7):482-96. https://doi.org/10.1038/nrm3823
  44. Wang LY, Tang ZJ, Han YZ. Neuroprotective effects of caffeic acid phenethyl ester against sevofluraneinduced neuronal degeneration in the hippocampus of neonatal rats involve MAPK and PI3K/Akt signaling pathways. Mol Med Rep. 2016;14(4):3403-12. https://doi.org/10.3892/mmr.2016.5586
  45. Tempark T, Chatproedprai S, Wananukul S. Localized contact dermatitis from Ferula assa-foetida oleo-gumresin essential oil, a traditional topical preparation for stomach ache and flatulence. Indian J Dermatol Venereol Leprol. 2016;82(4):467.
  46. Hadavand Mirzaei H, Hasanloo T. Assessment of chemical composition of essential oil of Ferula assafoetida oleo-gum-resin from two different sites of Yazd province in center of Iran. Research Journal of Pharmacognosy. 2014;1(2):51-4.
  47. Li RW, David Lin G, Myers SP, Leach DN. Anti-inflammatory activity of Chinese medicinal vine plants. J Ethnopharmacol. 2003;85(1):61-7. https://doi.org/10.1016/S0378-8741(02)00339-2

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