Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Recombinant Adeno-Associated Virus Expressing Truncated IK Cytokine Diminishes the Symptoms of Inflammatory Arthritis

  • Choi, Seulgi (Department of Biotechnology, The Catholic University of Korea) ;
  • Park, Hyelim (Department of Biotechnology, The Catholic University of Korea) ;
  • Minelko, Marstella (Department of Biotechnology, The Catholic University of Korea) ;
  • Kim, Eun-Kyung (The Rheumatism Research Center, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea) ;
  • Cho, Mi-Ra (The Rheumatism Research Center, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea) ;
  • Nam, Jae-Hwan (Department of Biotechnology, The Catholic University of Korea)
  • Received : 2017.05.08
  • Accepted : 2017.06.25
  • Published : 2017.10.28
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Abstract

IK can downregulate interferon-gamma-induced major histocompatibility complex (MHC) class II expression through the MHC class II transactivator, which suggests that IK can inhibit the interactions between immune cells. We delivered adeno-associated virus serotype 2 (AAV2) encoding the genes for truncated IK (tIK) or green fluorescent protein (GFP) to DBA1/J mice via intravenous injection. Seven weeks after injection, collagen-induced arthritis was induced in the AAV2-treated mice. AAV2-tIK injection reduced the severity of arthritis and the percentage of pathogenic Th17 cells compared with AAV2-GFP injection. These results suggest a novel gene therapy strategy for treatment of inflammatory arthritis.

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References

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