Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Oleaginous Yeast Rhodosporidium toruloides as a Tool for Rapid Evaluation of Anti-Obesity Candidates: Inhibitory Effect of Persimmon Leaf Fermentate on Lipid Accumulation

  • Lee, Nam Keun (Department of Oriental Medicine Resources, Chonbuk National University)
  • Received : 2017.07.26
  • Accepted : 2017.09.04
  • Published : 2017.10.28
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to examine the efficiency of Rhodosporidium toruloides as a new tool to evaluate the triglyceride (TG) reduction effects of anti-obesity candidate materials. Unfermented and fermented persimmon leaf hot water extracts (UFPLE and FPLE) were used as anti-obesity agents. The content of TG in R. toruloides treated with FPLE was less than those with UFPLE by about 11% (p < 0.05) relative to the control (R. toruloides incubated in YPD medium without the agents). Fat reduction in 3T3-L1 cells achieved by FPLE was about 13% higher than that achieved by UFPLE.

Keywords

References

  1. Martins S, Mussatto SI, Martinez-Avila G, Montanez-Saenz J, Aguilar CN, Teixeira JA. 2011. Bioactive phenolic compounds: production and extraction by solid-state fermentation. A review. Biotechnol. Adv. 29: 365-373. https://doi.org/10.1016/j.biotechadv.2011.01.008
  2. Madeira Jr JV, Teixeira CB, Macedo GA. 2015. Biotransformation and bioconversion of phenolic compounds obtainment: an overview. Crit. Rev. Biotechnol. 35: 75-81. https://doi.org/10.3109/07388551.2013.803020
  3. Lee NK, Jeong JH, Oh J, Kim Y, Ha YS, Jeong YS. 2015. Conversion of flavonols kaempferol and quercetin in mulberry (Morus alba L.) leaf using plant-fermenting Lactobacillus plantarum. J. Food Biochem. 39: 765-770. https://doi.org/10.1111/jfbc.12176
  4. Lee NK, Nyakudya E, Jeong YS. 2016. Bioconversion of Platycodon grandiflorum saponins by the platycodon D-converting microorganism, yeast Cyberlindnera fabianii. J. Food Biochem. 40: 358-365. https://doi.org/10.1111/jfbc.12217
  5. Butt MS, Sultan MT, Aziz M, Naz A, Ahmed W, Kumar N, et al. 2015. Persimmon (Diospyros kaki) fruit: hidden phytochemicals and health claims. EXCLI J. 14: 542-561.
  6. Singh S, Joshi H. 2011. Diospyros kaki (Ebenaceae): a review. Asian J. Res. Pharm. Sci. 1: 55-58.
  7. Kim KS, Lee DS, Kim DC, Yoon CS, Ko W, Oh H, et al. 2016. Anti-inflammatory effects and mechanisms of action of coussaric and betulinic acids isolated from Diospyros kaki in lipopolysaccharide-stimulated RAW 264.7 macrophages. Molecules 21: E1206. https://doi.org/10.3390/molecules21091206
  8. Yoo KH, Jeong JM. 2009. Antioxidative and antiallergic effect of persimmon leaf extracts. J. Korean Soc. Food Sci. Nutr. 38: 1691-1698. https://doi.org/10.3746/jkfn.2009.38.12.1691
  9. Jung UJ, Park YB, Kim SR, Choi MS. 2012. Supplementation of persimmon leaf ameliorates hyperglycemia, dyslipidemia and hepatic fat accumulation in type 2 diabetic mice. PLoS One 7: e49030. https://doi.org/10.1371/journal.pone.0049030
  10. Sancheti S, Sancheti S, Bafna M, Lee SH, Seo SY. 2011. Persimmon leaf (Diospyros kaki), a potent ${\alpha}$-glucosidase inhibitor and antioxidant: alleviation of postprandial hyperglycemia in normal and diabetic rats. J. Med. Plants Res. 5: 1652-1658.
  11. Jung UJ, Lee JS, Bok SH, Choi MS. 2011. Effects of extracts of persimmon leaf, buckwheat leaf, and Chinese matrimony vine leaf on body fat and lipid metabolism in rats. J. Korean Soc. Food Sci. Nutr. 40: 1215-1226. https://doi.org/10.3746/jkfn.2011.40.9.1215
  12. Mure K, Takeshita T, Morioka I, Arita M. 2007. Effects of kakisu (persimmon vinegar) on plasma antioxidant power and urinary 8-isoprostane level. Nihon Eiseigaku Zasshi 62: 32-38. https://doi.org/10.1265/jjh.62.32
  13. Moon YJ, Cha YS. 2008. Effects of persimmon-vinegar on lipid metabolism and alcohol clearance in chronic alcoholfed rats. J. Med. Food 11: 38-45. https://doi.org/10.1089/jmf.2007.071
  14. Lee HM, Park MY, Kim J, Shin JH, Park KS, Kwon O. 2016. Persimmon vinegar and its fractions protect against alcoholinduced hepatic injury in rats through the suppression of CYP2E1 expression. Pharm. Biol. 54: 2437-2442. https://doi.org/10.3109/13880209.2016.1158842
  15. Seo SY, Ahn MS, Chio SR, Song EJ, Choi MK, Yoo SM, et al. 2015. Characteristics of persimmon juice fermented with kimchi lactic acid bacteria. Korean J. Food Nutr. 28: 16-23. https://doi.org/10.9799/ksfan.2015.28.1.016
  16. Kang WW, Kim GY, Kim J K, Oh SL. 2000. Quality characteristics of bread added persimmon leaves powder. Korean J. Soc. Food Sci. 16: 336-341.
  17. Bae JH, Woo HS, Chio HJ, Choi C. 2001. Qualities of bread added with Korean persimmon (Diospyros kaki L. folium) leaf powder. J. Korean Soc. Food Sci. Nutr. 30: 882-887.
  18. Park CS. 2003. Functional properties of tea-fungus beverage. Korean J. Food Preserv. 10: 241-245.
  19. Xue YL, Miyakawa T, Hayashi Y, Okamoto K, Hu F, Mitani N, et al. 2011. Isolation and tyrosinase inhibitory effects of polyphenols from the leaves of persimmon, Diospyros kaki. J. Agric. Food Chem. 59: 6011-6017. https://doi.org/10.1021/jf200940h
  20. Kawser Hossain M, Abdal Dayem A, Han J, Yin Y, Kim K, Kumar Saha S, et al. 2016. Molecular mechanisms of the anti-obesity and anti-diabetic properties of flavonoids. Int. J. Mol. Sci. 17: 569. https://doi.org/10.3390/ijms17040569
  21. Sha Q. 2013. A comparative study on four oleaginous yeasts on their lipid accumulating capacity. Master's Degree, Swedish University of Agricultural Sciences, Department of Microbiology, Uppsala, Sweden.
  22. Lee Y, Oh J, Lee H, Lee NK, Jeong DY, Jeong YS. 2015. Lactic acid bacteria-mediated fermentation of Cudrania tricuspidata leaf extract improves its antioxidative activity, osteogenic effects, and anti-adipogenic effects.Biotechnol. Bioproc. Eng. 20: 861-870. https://doi.org/10.1007/s12257-015-0302-y
  23. Iwashita K, Yamaki K, Tsushida T. 2001. Effect of flavonoids on the differentiation of 3T3-L1 adipocytes. Food Sci. Technol. Res. 7: 154-160. https://doi.org/10.3136/fstr.7.154
  24. Zebisch K, Voigt V, Wabitsch M, Brandsch M. 2012. Protocol for effective differentiation of 3T3-L1 cells to adipocytes. Anal. Biochem. 425: 88-90. https://doi.org/10.1016/j.ab.2012.03.005
  25. Capus A, Monnerat M, Ribeiro LC, de Souza W, Martins JL, Sant'Anna C. 2016. Application of high-content image analysis for quantitatively estimating lipid accumulation in oleaginous yeasts with potential for use in biodiesel production. Bioresour. Technol. 203: 309-317. https://doi.org/10.1016/j.biortech.2015.12.067
  26. Garay LA, Sitepu IR, Cajka T, Chandra I, Shi S, Lin T, et al. 2016. Eighteen new oleaginous yeast species. J. Ind. Microbiol. Biotechnol. 43: 887-900. https://doi.org/10.1007/s10295-016-1765-3
  27. Liu H, Zhao X, Wang F, Li Y, Jiang X, Ye M, et al. 2009. Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation. Yeast 26: 553-566. https://doi.org/10.1002/yea.1706
  28. Zhu Z, Zhang S, Liu H, Shen H, Lin X, Yang F, et al. 2012. A multi-omic map of the lipid-producing yeast Rhodosporidium toruloides. Nat. Commun. 3: 1112. https://doi.org/10.1038/ncomms2112
  29. Li Y, Zhao Z, Bai F. 2007. High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture. Enzyme Microb. Technol. 41: 312-317. https://doi.org/10.1016/j.enzmictec.2007.02.008
  30. Patel A, Pruthi V, Singh RP, Pruthi PA. 2015. Synergistic effect of fermentable and non-fermentable carbon sources enhances TAG accumulation in oleaginous yeast Rhodosporidium kratochvilovae HIMPA1. Bioresour. Technol. 188: 136-144. https://doi.org/10.1016/j.biortech.2015.02.062
  31. Kang ES, Ham SA, Hwang JS, Lee CK, Seo HG. 2013. Effects of Garcinia cambogia extract on the adipogenic differentiation and lipotoxicity. Korean J. Food Sci. Anim. Resour. 33: 411-416. https://doi.org/10.5851/kosfa.2013.33.3.411
  32. Kang ES, Hwang JS, Kim MH, Kim HJ, Lee CK, Seo HG. 2013. Effects of Garcinia cambogia extract-containing dip-sauce for meat on lipid accumulation and body weight reduction in rats fed high-fat diet. Korean J. Food Sci. Anim. Resour. 33: 276-280. https://doi.org/10.5851/kosfa.2013.33.2.276
  33. Kimura K, Yamaoka M, Kamisaka Y. 2004. Rapid estimation of lipid in oleaginous fungi and yeasts using Nile red fluorescence. J. Microbiol. Methods 56: 331-338. https://doi.org/10.1016/j.mimet.2003.10.018
  34. Swierczynski J, Zabrocka L, Goyke E, Raczynska S, Adamonis W, Sledzinski Z. 2003. Enhanced glycerol 3- phosphate dehydrogenase activity in adipose tissue of obese humans. Mol. Cell. Biochem. 254: 55-59. https://doi.org/10.1023/A:1027332523114
  35. Zhu Z, Ding Y, Gong Z, Yang L, Zhang S, Zhang C, et al. 2015. Dynamics of the lipid droplet proteome of the oleaginous yeast Rhodosporidium toruloides. Eukaryot. Cell 14: 252-264. https://doi.org/10.1128/EC.00141-14

Cited by

  1. Anti-Obesity Effect of Red Radish Coral Sprout Extract by Inhibited Triglyceride Accumulation in a Microbial Evaluation System and in High-Fat Diet-Induced Obese Mice vol.28, pp.3, 2017, https://doi.org/10.4014/jmb.1802.02005