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Identification and FT-IR Spectrum Analysis of Lichens on Flagpole Support in Beopjusa Temple

법주사 당간지주 지의류의 동정 및 FT-IR 스펙트럼 특성 분석

  • Kim, Young Hee (Restoration Technology Division, National Research Institute of Cultural Heritage) ;
  • Lee, Jeung Min (Restoration Technology Division, National Research Institute of Cultural Heritage) ;
  • Choie, Myoungju (Conservation Science Division, National Research Institute of Cultural Heritage) ;
  • Hong, Jin Young (Restoration Technology Division, National Research Institute of Cultural Heritage) ;
  • Jo, Chang Wook (Restoration Technology Division, National Research Institute of Cultural Heritage) ;
  • Kim, Soo Ji (Restoration Technology Division, National Research Institute of Cultural Heritage) ;
  • Jeong, So Young (Restoration Technology Division, National Research Institute of Cultural Heritage)
  • 김영희 (국립문화재연구소 복원기술연구실) ;
  • 이정민 (국립문화재연구소 복원기술연구실) ;
  • 최명주 (국립문화재연구소 보존과학연구실) ;
  • 홍진영 (국립문화재연구소 복원기술연구실) ;
  • 조창욱 (국립문화재연구소 복원기술연구실) ;
  • 김수지 (국립문화재연구소 복원기술연구실) ;
  • 정소영 (국립문화재연구소 복원기술연구실)
  • Received : 2017.09.14
  • Accepted : 2017.10.17
  • Published : 2017.10.20

Abstract

This study was conducted to investigate lichen as a typical biomass damage on the surfaces of flagpole-supporting stones in the Beopjusa temple. The lichens present on the flagpole-supporting stones were limited to five species. Two dominant lichen species were identified: Aspicilia sp. and Pertusaria flavicans. One foliose species and one fruticose species, which are rarely observed on crustose lichens, were identified as Xanthoparmelia conspersa and Ramalina sekika, respectively. The lichen inhabiting the black algae layer was confirmed as Leprocaulon textum. ATR-FTIR was performed to analyze the secondary metabolites synthesized by the lichens. By comparing the FTIR spectra of Xanthoparmelia conspersa and Ramalina sekika, the synthesized organic acids were confirmed to differ from each other. Furthermore, the spectral changes and characteristics due to functional groups in the molecules were confirmed.

본 연구에서는 법주사 당간지주의 석재 표면에 서식하는 대표적인 생물피해 요인인 지의류에 대하여 조사하였다. 당간지주의 지의류는 5종으로 한정할 수 있다. 그 중 우점하는 2종의 고착상지의류는 Aspicilia sp.와 Pertusaria flavicans이며, 고착상지의류 위에 드물게 관찰되는 엽상지의류 1종과 수지상지의류 1종은 Xanthoparmelia conspersa와 Ramalina sekika로 동정되었다. 그리고 흑색조류층을 따라 서식하는 지의류는 Leprocaulon textum으로 확인되었다. 지의류가 생산하는 2차 대사산물을 분석하기 위하여 ATR FT-IR을 적용하였다. Xanthoparmelia conspersa와 Ramalina sekika의 FT-IR 스펙트럼을 비교해 본 결과, cyclic carbohydrate에 기인하는 C-O-C 결합과 carboxylic acid에서 기인하는 FT-IR 스펙트럼의 흡수피크가 특징적으로 관찰되어 이들이 생산하는 지의산이 서로 다르다는 것을 확인할 수 있었으며, stictic acid와 sekikaic acid가 Xanthoparmelia conspersa와 Ramalina sekika를 대표하는 지의산으로 화학구조에서 작용기의 차이에 의한 스펙트럼의 변화 및 특성을 확인할 수 있었다.

Keywords

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