CELLMED (셀메드)

Cellmed Orthocellular Medicine and Pharmaceutical Association (셀메드 세포교정의약학회)
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Validation of chrysophanol and cordycepin as marker compounds for standardization of a new herbal mixture AST2017-01

  • Jang, Jae-Bum (Department of Pharmaceutical Engineering, Hoseo University) ;
  • Kim, Tae-Young (Gahwa Well Food Co.) ;
  • Jeong, Hyun-Ja (Department of Food Science & Technology and Research Institute for Basic Science, Hoseo University) ;
  • Kim, Hyung-Min (Department of Pharmacology, College of Korean Medicine, Kyung Hee University)
  • Received : 2017.06.27
  • Accepted : 2017.08.21
  • Published : 2017.08.31
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Abstract

Rumex crispus (RC) or Cordyceps militaris (CM) has been used traditionally to treat various diseases and has been also consumed as a functional food made by humanitas medicine concept. We prepared a new herbal mixture, AST2017-01 which is mainly composed of processed (Beopje)-RC (P-RC) and -CM (P-CM). This study aims to validate marker compounds (chrysophanol and cordycepin) in P-RC and P-CM and water extracted-RC and -CM using liquid chromatography-tandem mass spectrometry. In addition, we analyzed contents of chrysophanol and cordycepin in AST2017-01. The linarites of chrysophanol and cordycepin were obtained in calibration curve with a coefficient of correlation of 0.999. The results showed that the concentrations of chrysophanol and cordycepin in P-RC and P-CM were almost 1.70 and 1.23 fold higher than that in RC and CM, respectively. Furthermore, contents of chrysophanol and cordycepin in the AST2017-01 are approximately 0.13% and 0.028%, respectively. In conclusion, these results indicate that chrysophanol and cordycepin were validated as marker compounds in the AST2017-01.

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References

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